Antibody-functionalized MXene-based electrochemical biosensor for point-of-care detection of vitamin D deficiency
Abstract Clinical studies routinely show that individuals suffer from vitamin D deficiency, which can result in health complications that include cardiovascular disease, autoimmune disorders, neurodegenerative diseases, and different skeletal deformities. Given its integral role in homeostasis and c...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-02-01
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| Series: | Communications Materials |
| Online Access: | https://doi.org/10.1038/s43246-025-00756-9 |
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| author | Sharat Chandra Barman Yuming Jin Jehad K. El-Demellawi Simil Thomas Nimer Wehbe Yongjiu Lei Mrinal Kanti Hota Xiangming Xu Erol A. Hasan Omar F. Mohammed Osman M. Bakr Dana Alsulaiman Husam N. Alshareef |
| author_facet | Sharat Chandra Barman Yuming Jin Jehad K. El-Demellawi Simil Thomas Nimer Wehbe Yongjiu Lei Mrinal Kanti Hota Xiangming Xu Erol A. Hasan Omar F. Mohammed Osman M. Bakr Dana Alsulaiman Husam N. Alshareef |
| author_sort | Sharat Chandra Barman |
| collection | DOAJ |
| description | Abstract Clinical studies routinely show that individuals suffer from vitamin D deficiency, which can result in health complications that include cardiovascular disease, autoimmune disorders, neurodegenerative diseases, and different skeletal deformities. Given its integral role in homeostasis and connection to many pathologies, early diagnosis of vitamin D deficiency is crucial. However, monitoring vitamin D levels is challenging, particularly in remote regions, due to the cost, time, and complexity of existing methods. Here, we develop an electrochemical biosensor for vitamin D based on antibody-functionalized MXenes, offering clinically relevant sensitivity, specificity, and amenability for point-of-care testing. Ti3C2Tx MXene nanosheets are amine-functionalized by electrostatically-driven modification with polyethylenimine, whose functionalities are then used for covalent conjugation of anti-vitamin D antibodies via glutaraldehyde chemistry. This platform achieves a detection limit of 1 pg mL−1 with a dynamic range (0.1–500 ng mL−1) that covers clinically relevant deficiency, insufficiency, sufficiency, and toxicity. |
| format | Article |
| id | doaj-art-cd426be30b6f45a0aaebef104f62b8ce |
| institution | DOAJ |
| issn | 2662-4443 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Materials |
| spelling | doaj-art-cd426be30b6f45a0aaebef104f62b8ce2025-08-20T03:13:12ZengNature PortfolioCommunications Materials2662-44432025-02-016111110.1038/s43246-025-00756-9Antibody-functionalized MXene-based electrochemical biosensor for point-of-care detection of vitamin D deficiencySharat Chandra Barman0Yuming Jin1Jehad K. El-Demellawi2Simil Thomas3Nimer Wehbe4Yongjiu Lei5Mrinal Kanti Hota6Xiangming Xu7Erol A. Hasan8Omar F. Mohammed9Osman M. Bakr10Dana Alsulaiman11Husam N. Alshareef12Materials Science and Engineering, Physical Science and Engineering (PSE) Division, King Abdullah University of Science and Technology (KAUST)Materials Science and Engineering, Physical Science and Engineering (PSE) Division, King Abdullah University of Science and Technology (KAUST)Center for Renewable Energy and Storage Technologies (CREST), King Abdullah University of Science and Technology (KAUST)Materials Science and Engineering, Physical Science and Engineering (PSE) Division, King Abdullah University of Science and Technology (KAUST)Core Labs, King Abdullah University of Science and Technology (KAUST)Materials Science and Engineering, Physical Science and Engineering (PSE) Division, King Abdullah University of Science and Technology (KAUST)Materials Science and Engineering, Physical Science and Engineering (PSE) Division, King Abdullah University of Science and Technology (KAUST)Materials Science and Engineering, Physical Science and Engineering (PSE) Division, King Abdullah University of Science and Technology (KAUST)Materials Science and Engineering, Physical Science and Engineering (PSE) Division, King Abdullah University of Science and Technology (KAUST)Materials Science and Engineering, Physical Science and Engineering (PSE) Division, King Abdullah University of Science and Technology (KAUST)Materials Science and Engineering, Physical Science and Engineering (PSE) Division, King Abdullah University of Science and Technology (KAUST)Materials Science and Engineering, Physical Science and Engineering (PSE) Division, King Abdullah University of Science and Technology (KAUST)Materials Science and Engineering, Physical Science and Engineering (PSE) Division, King Abdullah University of Science and Technology (KAUST)Abstract Clinical studies routinely show that individuals suffer from vitamin D deficiency, which can result in health complications that include cardiovascular disease, autoimmune disorders, neurodegenerative diseases, and different skeletal deformities. Given its integral role in homeostasis and connection to many pathologies, early diagnosis of vitamin D deficiency is crucial. However, monitoring vitamin D levels is challenging, particularly in remote regions, due to the cost, time, and complexity of existing methods. Here, we develop an electrochemical biosensor for vitamin D based on antibody-functionalized MXenes, offering clinically relevant sensitivity, specificity, and amenability for point-of-care testing. Ti3C2Tx MXene nanosheets are amine-functionalized by electrostatically-driven modification with polyethylenimine, whose functionalities are then used for covalent conjugation of anti-vitamin D antibodies via glutaraldehyde chemistry. This platform achieves a detection limit of 1 pg mL−1 with a dynamic range (0.1–500 ng mL−1) that covers clinically relevant deficiency, insufficiency, sufficiency, and toxicity.https://doi.org/10.1038/s43246-025-00756-9 |
| spellingShingle | Sharat Chandra Barman Yuming Jin Jehad K. El-Demellawi Simil Thomas Nimer Wehbe Yongjiu Lei Mrinal Kanti Hota Xiangming Xu Erol A. Hasan Omar F. Mohammed Osman M. Bakr Dana Alsulaiman Husam N. Alshareef Antibody-functionalized MXene-based electrochemical biosensor for point-of-care detection of vitamin D deficiency Communications Materials |
| title | Antibody-functionalized MXene-based electrochemical biosensor for point-of-care detection of vitamin D deficiency |
| title_full | Antibody-functionalized MXene-based electrochemical biosensor for point-of-care detection of vitamin D deficiency |
| title_fullStr | Antibody-functionalized MXene-based electrochemical biosensor for point-of-care detection of vitamin D deficiency |
| title_full_unstemmed | Antibody-functionalized MXene-based electrochemical biosensor for point-of-care detection of vitamin D deficiency |
| title_short | Antibody-functionalized MXene-based electrochemical biosensor for point-of-care detection of vitamin D deficiency |
| title_sort | antibody functionalized mxene based electrochemical biosensor for point of care detection of vitamin d deficiency |
| url | https://doi.org/10.1038/s43246-025-00756-9 |
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