Site-Specific Analysis of Inflammatory Markers in Discoid Lupus Erythematosus Skin
Prior studies identified T cells, B cells, and macrophages in the inflammatory infiltrate and up-regulation of their protein products in discoid lupus erythematosus (DLE) skin; however, they lacked rigorous analyses to define their specific locations in skin. Thus, we compared expressions of selecte...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/925805 |
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| author | Ryan B. Thorpe Anna Gray Kirthi R. Kumar Joseph S. Susa Benjamin F. Chong |
| author_facet | Ryan B. Thorpe Anna Gray Kirthi R. Kumar Joseph S. Susa Benjamin F. Chong |
| author_sort | Ryan B. Thorpe |
| collection | DOAJ |
| description | Prior studies identified T cells, B cells, and macrophages in the inflammatory infiltrate and up-regulation of their protein products in discoid lupus erythematosus (DLE) skin; however, they lacked rigorous analyses to define their specific locations in skin. Thus, we compared expressions of selected T cell, B cell, and macrophage markers in five areas of DLE, psoriasis, and normal skin. Immunostainings for CD3, CD4, CD8, CD20, CD68, CXCR3, CXCL10, and TIA-1 were performed in biopsies of 23 DLE lesional skin, 11 psoriasis lesional skin, and 5 normal skin. Three independent observers used a graded scale to rate each marker’s presence in the epidermis, dermatoepidermal junction (DEJ), perivascular area, periadnexal area, and deep dermis. DLE lesional skin contained an increased abundance of CD3+, CD8+, and CD68+ cells at the DEJ, and CD20+ and CD68+ cells in the periadnexal area versus psoriasis and normal skin. CXCR3, CXCL10, and TIA-1 were elevated in periadnexal sites of DLE lesional skin versus psoriasis lesional skin. The aggregation of T cells, B cells, macrophages, and their protein products (CXCR3, CXCL10, and TIA-1) in the DEJ and periadnexal area of DLE lesional skin may contribute to the pathology of DLE through a coordinated, sophisticated process. |
| format | Article |
| id | doaj-art-cd39f58067784f7f91733f3a99cd3032 |
| institution | OA Journals |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-cd39f58067784f7f91733f3a99cd30322025-08-20T02:19:51ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/925805925805Site-Specific Analysis of Inflammatory Markers in Discoid Lupus Erythematosus SkinRyan B. Thorpe0Anna Gray1Kirthi R. Kumar2Joseph S. Susa3Benjamin F. Chong4Department of Dermatology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390-9069, USADepartment of Pathology, University of Texas Southwestern Medical Center, Dallas, TX 75390-9072, USADepartment of Pathology, University of Texas Southwestern Medical Center, Dallas, TX 75390-9072, USADepartment of Dermatology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390-9069, USADepartment of Dermatology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390-9069, USAPrior studies identified T cells, B cells, and macrophages in the inflammatory infiltrate and up-regulation of their protein products in discoid lupus erythematosus (DLE) skin; however, they lacked rigorous analyses to define their specific locations in skin. Thus, we compared expressions of selected T cell, B cell, and macrophage markers in five areas of DLE, psoriasis, and normal skin. Immunostainings for CD3, CD4, CD8, CD20, CD68, CXCR3, CXCL10, and TIA-1 were performed in biopsies of 23 DLE lesional skin, 11 psoriasis lesional skin, and 5 normal skin. Three independent observers used a graded scale to rate each marker’s presence in the epidermis, dermatoepidermal junction (DEJ), perivascular area, periadnexal area, and deep dermis. DLE lesional skin contained an increased abundance of CD3+, CD8+, and CD68+ cells at the DEJ, and CD20+ and CD68+ cells in the periadnexal area versus psoriasis and normal skin. CXCR3, CXCL10, and TIA-1 were elevated in periadnexal sites of DLE lesional skin versus psoriasis lesional skin. The aggregation of T cells, B cells, macrophages, and their protein products (CXCR3, CXCL10, and TIA-1) in the DEJ and periadnexal area of DLE lesional skin may contribute to the pathology of DLE through a coordinated, sophisticated process.http://dx.doi.org/10.1155/2014/925805 |
| spellingShingle | Ryan B. Thorpe Anna Gray Kirthi R. Kumar Joseph S. Susa Benjamin F. Chong Site-Specific Analysis of Inflammatory Markers in Discoid Lupus Erythematosus Skin The Scientific World Journal |
| title | Site-Specific Analysis of Inflammatory Markers in Discoid Lupus Erythematosus Skin |
| title_full | Site-Specific Analysis of Inflammatory Markers in Discoid Lupus Erythematosus Skin |
| title_fullStr | Site-Specific Analysis of Inflammatory Markers in Discoid Lupus Erythematosus Skin |
| title_full_unstemmed | Site-Specific Analysis of Inflammatory Markers in Discoid Lupus Erythematosus Skin |
| title_short | Site-Specific Analysis of Inflammatory Markers in Discoid Lupus Erythematosus Skin |
| title_sort | site specific analysis of inflammatory markers in discoid lupus erythematosus skin |
| url | http://dx.doi.org/10.1155/2014/925805 |
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