Identification and Characterization of the RNA Modifying Factors PUS7 and WTAP as Key Components for the Control of Tumor Biological Processes in Renal Cell Carcinomas
Current research discusses the putative importance of RNA modification in tumor diseases. These RNA modifications include predominantly pseudouridinylation, ortho-methylations on the ribose residues, as well as methylations on the organic bases. Such chemical modifications directly influence fundame...
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MDPI AG
2025-04-01
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| author | Tim Hohmann Urszula Hohmann Faramarz Dehghani Olaf Grisk Simon Jasinski-Bergner |
| author_facet | Tim Hohmann Urszula Hohmann Faramarz Dehghani Olaf Grisk Simon Jasinski-Bergner |
| author_sort | Tim Hohmann |
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| description | Current research discusses the putative importance of RNA modification in tumor diseases. These RNA modifications include predominantly pseudouridinylation, ortho-methylations on the ribose residues, as well as methylations on the organic bases. Such chemical modifications directly influence fundamental properties such as transcript stability, alternative splicing, and translation efficiency, all of which are basic requirements for (tumor) cell proliferation, cell metabolism, cell migration, apoptosis resistance, etc. In this comparative study, the two RNA-modifying factors, pseudouridine synthase 7 (PUS7, RNA pseudouridinylation) and WT1-associated protein (WTAP, m6A RNA methylation), were identified using data from human renal cell carcinoma (RCC) tumors. PUS7 and WTAP showed a statistically significant correlation with relevant proliferation and prognosis markers such as CXCR4, TP53, PTEN, and NRAS, as well as with the two tumor immune checkpoints HLA-G and LGALS9 and were directly associated with a statistically significant effect on overall survival. Furthermore, comparative analyses also identified further putative target mRNAs of importance for tumor biology of PUS7 and WTAP. In particular, components with direct relevance for mitosis, the cell cycle, and cell division, as well as the WNT pathway, were identified. |
| format | Article |
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| issn | 1467-3037 1467-3045 |
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| publishDate | 2025-04-01 |
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| spelling | doaj-art-cd379a008d5e4af390840d9ef3e6c6ce2025-08-20T02:28:19ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452025-04-0147426610.3390/cimb47040266Identification and Characterization of the RNA Modifying Factors PUS7 and WTAP as Key Components for the Control of Tumor Biological Processes in Renal Cell CarcinomasTim Hohmann0Urszula Hohmann1Faramarz Dehghani2Olaf Grisk3Simon Jasinski-Bergner4Department of Anatomy and Cell Biology, Medical Faculty, Martin Luther University Halle-Wittenberg, Grosse Steinstrasse 52, 06108 Halle (Saale), GermanyDepartment of Anatomy and Cell Biology, Medical Faculty, Martin Luther University Halle-Wittenberg, Grosse Steinstrasse 52, 06108 Halle (Saale), GermanyDepartment of Anatomy and Cell Biology, Medical Faculty, Martin Luther University Halle-Wittenberg, Grosse Steinstrasse 52, 06108 Halle (Saale), GermanyInstitute of Physiology, Brandenburg Medical School (MHB), Theodor Fontane, Hochstraße 29 Haus 11 2.OG, 14770 Brandenburg an der Havel, GermanyInstitute of Physiology, Brandenburg Medical School (MHB), Theodor Fontane, Hochstraße 29 Haus 11 2.OG, 14770 Brandenburg an der Havel, GermanyCurrent research discusses the putative importance of RNA modification in tumor diseases. These RNA modifications include predominantly pseudouridinylation, ortho-methylations on the ribose residues, as well as methylations on the organic bases. Such chemical modifications directly influence fundamental properties such as transcript stability, alternative splicing, and translation efficiency, all of which are basic requirements for (tumor) cell proliferation, cell metabolism, cell migration, apoptosis resistance, etc. In this comparative study, the two RNA-modifying factors, pseudouridine synthase 7 (PUS7, RNA pseudouridinylation) and WT1-associated protein (WTAP, m6A RNA methylation), were identified using data from human renal cell carcinoma (RCC) tumors. PUS7 and WTAP showed a statistically significant correlation with relevant proliferation and prognosis markers such as CXCR4, TP53, PTEN, and NRAS, as well as with the two tumor immune checkpoints HLA-G and LGALS9 and were directly associated with a statistically significant effect on overall survival. Furthermore, comparative analyses also identified further putative target mRNAs of importance for tumor biology of PUS7 and WTAP. In particular, components with direct relevance for mitosis, the cell cycle, and cell division, as well as the WNT pathway, were identified.https://www.mdpi.com/1467-3045/47/4/266RCCtranscriptomePUS7WTAPalternative splicingRNA modifications |
| spellingShingle | Tim Hohmann Urszula Hohmann Faramarz Dehghani Olaf Grisk Simon Jasinski-Bergner Identification and Characterization of the RNA Modifying Factors PUS7 and WTAP as Key Components for the Control of Tumor Biological Processes in Renal Cell Carcinomas Current Issues in Molecular Biology RCC transcriptome PUS7 WTAP alternative splicing RNA modifications |
| title | Identification and Characterization of the RNA Modifying Factors PUS7 and WTAP as Key Components for the Control of Tumor Biological Processes in Renal Cell Carcinomas |
| title_full | Identification and Characterization of the RNA Modifying Factors PUS7 and WTAP as Key Components for the Control of Tumor Biological Processes in Renal Cell Carcinomas |
| title_fullStr | Identification and Characterization of the RNA Modifying Factors PUS7 and WTAP as Key Components for the Control of Tumor Biological Processes in Renal Cell Carcinomas |
| title_full_unstemmed | Identification and Characterization of the RNA Modifying Factors PUS7 and WTAP as Key Components for the Control of Tumor Biological Processes in Renal Cell Carcinomas |
| title_short | Identification and Characterization of the RNA Modifying Factors PUS7 and WTAP as Key Components for the Control of Tumor Biological Processes in Renal Cell Carcinomas |
| title_sort | identification and characterization of the rna modifying factors pus7 and wtap as key components for the control of tumor biological processes in renal cell carcinomas |
| topic | RCC transcriptome PUS7 WTAP alternative splicing RNA modifications |
| url | https://www.mdpi.com/1467-3045/47/4/266 |
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