The Importance of the Prenyl Group in the Activities of Osthole in Enhancing Bone Formation and Inhibiting Bone Resorption In Vitro
Osteoporosis treatment always aimed at keeping the balance of bone formation and bone resorption. Recently, prenyl group in natural products has been proposed as an active group to enhance the osteogenesis process. Osthole has both the prenyl group and bone-protective activities, but the relationshi...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | International Journal of Endocrinology |
| Online Access: | http://dx.doi.org/10.1155/2014/921954 |
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| author | Yuan-Kun Zhai Ya-Lei Pan Yin-Bo Niu Chen-Rui Li Xiang-Long Wu Wu-Tu Fan Ting-Li Lu Qi-Bing Mei Cory J. Xian |
| author_facet | Yuan-Kun Zhai Ya-Lei Pan Yin-Bo Niu Chen-Rui Li Xiang-Long Wu Wu-Tu Fan Ting-Li Lu Qi-Bing Mei Cory J. Xian |
| author_sort | Yuan-Kun Zhai |
| collection | DOAJ |
| description | Osteoporosis treatment always aimed at keeping the balance of bone formation and bone resorption. Recently, prenyl group in natural products has been proposed as an active group to enhance the osteogenesis process. Osthole has both the prenyl group and bone-protective activities, but the relationship is still unknown. In this study we found that osthole exerted a potent ability to promote proliferation and osteogenic function of rat bone marrow stromal cells and osteoblasts, including improved cell viability, alkaline phosphatase activity, enhanced secretion of collagen-I, bone morphogenetic protein-2, osteocalcin and osteopontin, stimulated mRNA expression of insulin-like growth factor-1, runt-related transcription factor-2, osterix, OPG (osteoprotegerin), RANKL (receptor activator for nuclear factor-κB ligand), and the ratio of OPG/RANKL, as well as increasing the formation of mineralized nodules. However, 7-methoxycoumarin had no obvious effects. Osthole also inhibited osteoclastic bone resorption to a greater extent than 7-methoxycoumarin, as shown by a lower tartrate-resistant acid phosphatase activity and lower number and smaller area of resorption pits. Our findings demonstrate that osthole could be a potential agent to stimulate bone formation and inhibit bone resorption, and the prenyl group plays an important role in these bone-protective effects. |
| format | Article |
| id | doaj-art-cd339693d49d4e8e988a53c7b58d5e1e |
| institution | Kabale University |
| issn | 1687-8337 1687-8345 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Endocrinology |
| spelling | doaj-art-cd339693d49d4e8e988a53c7b58d5e1e2025-02-03T06:41:58ZengWileyInternational Journal of Endocrinology1687-83371687-83452014-01-01201410.1155/2014/921954921954The Importance of the Prenyl Group in the Activities of Osthole in Enhancing Bone Formation and Inhibiting Bone Resorption In VitroYuan-Kun Zhai0Ya-Lei Pan1Yin-Bo Niu2Chen-Rui Li3Xiang-Long Wu4Wu-Tu Fan5Ting-Li Lu6Qi-Bing Mei7Cory J. Xian8Key Laboratory for Space Bioscience and Biotechnology, College of Life Science, Northwestern Polytechnical University, Xi’an, Shaanxi 710072, ChinaKey Laboratory for Space Bioscience and Biotechnology, College of Life Science, Northwestern Polytechnical University, Xi’an, Shaanxi 710072, ChinaKey Laboratory for Space Bioscience and Biotechnology, College of Life Science, Northwestern Polytechnical University, Xi’an, Shaanxi 710072, ChinaKey Laboratory for Space Bioscience and Biotechnology, College of Life Science, Northwestern Polytechnical University, Xi’an, Shaanxi 710072, ChinaKey Laboratory for Space Bioscience and Biotechnology, College of Life Science, Northwestern Polytechnical University, Xi’an, Shaanxi 710072, ChinaKey Laboratory for Space Bioscience and Biotechnology, College of Life Science, Northwestern Polytechnical University, Xi’an, Shaanxi 710072, ChinaKey Laboratory for Space Bioscience and Biotechnology, College of Life Science, Northwestern Polytechnical University, Xi’an, Shaanxi 710072, ChinaKey Laboratory for Space Bioscience and Biotechnology, College of Life Science, Northwestern Polytechnical University, Xi’an, Shaanxi 710072, ChinaSansom Institute for Health Research, School of Pharmacy and Medical Sciences, University of South Australia, Adelaide, SA 5001, AustraliaOsteoporosis treatment always aimed at keeping the balance of bone formation and bone resorption. Recently, prenyl group in natural products has been proposed as an active group to enhance the osteogenesis process. Osthole has both the prenyl group and bone-protective activities, but the relationship is still unknown. In this study we found that osthole exerted a potent ability to promote proliferation and osteogenic function of rat bone marrow stromal cells and osteoblasts, including improved cell viability, alkaline phosphatase activity, enhanced secretion of collagen-I, bone morphogenetic protein-2, osteocalcin and osteopontin, stimulated mRNA expression of insulin-like growth factor-1, runt-related transcription factor-2, osterix, OPG (osteoprotegerin), RANKL (receptor activator for nuclear factor-κB ligand), and the ratio of OPG/RANKL, as well as increasing the formation of mineralized nodules. However, 7-methoxycoumarin had no obvious effects. Osthole also inhibited osteoclastic bone resorption to a greater extent than 7-methoxycoumarin, as shown by a lower tartrate-resistant acid phosphatase activity and lower number and smaller area of resorption pits. Our findings demonstrate that osthole could be a potential agent to stimulate bone formation and inhibit bone resorption, and the prenyl group plays an important role in these bone-protective effects.http://dx.doi.org/10.1155/2014/921954 |
| spellingShingle | Yuan-Kun Zhai Ya-Lei Pan Yin-Bo Niu Chen-Rui Li Xiang-Long Wu Wu-Tu Fan Ting-Li Lu Qi-Bing Mei Cory J. Xian The Importance of the Prenyl Group in the Activities of Osthole in Enhancing Bone Formation and Inhibiting Bone Resorption In Vitro International Journal of Endocrinology |
| title | The Importance of the Prenyl Group in the Activities of Osthole in Enhancing Bone Formation and Inhibiting Bone Resorption In Vitro |
| title_full | The Importance of the Prenyl Group in the Activities of Osthole in Enhancing Bone Formation and Inhibiting Bone Resorption In Vitro |
| title_fullStr | The Importance of the Prenyl Group in the Activities of Osthole in Enhancing Bone Formation and Inhibiting Bone Resorption In Vitro |
| title_full_unstemmed | The Importance of the Prenyl Group in the Activities of Osthole in Enhancing Bone Formation and Inhibiting Bone Resorption In Vitro |
| title_short | The Importance of the Prenyl Group in the Activities of Osthole in Enhancing Bone Formation and Inhibiting Bone Resorption In Vitro |
| title_sort | importance of the prenyl group in the activities of osthole in enhancing bone formation and inhibiting bone resorption in vitro |
| url | http://dx.doi.org/10.1155/2014/921954 |
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