Genome-Wide Identification and Functional Characterization of the BAHD Acyltransferase Gene Family in <i>Brassica napus</i> L.
The BAHD acyltransferase family plays a critical role in plant secondary metabolism by catalyzing acyl transfer reactions that are essential for synthesizing metabolites involved in environmental adaptation. However, systematic investigation of this superfamily in <i>Brassica napus</i> h...
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2025-07-01
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| author | Yuanyuan Liu Xingzhi Wei Yiwei Liu Yunshan Tang Shulin Shen Jie Xu Lulu Chen Cunmin Qu Huiyan Zhao Hai Du Huafang Wan Nengwen Yin Ti Zhang |
| author_facet | Yuanyuan Liu Xingzhi Wei Yiwei Liu Yunshan Tang Shulin Shen Jie Xu Lulu Chen Cunmin Qu Huiyan Zhao Hai Du Huafang Wan Nengwen Yin Ti Zhang |
| author_sort | Yuanyuan Liu |
| collection | DOAJ |
| description | The BAHD acyltransferase family plays a critical role in plant secondary metabolism by catalyzing acyl transfer reactions that are essential for synthesizing metabolites involved in environmental adaptation. However, systematic investigation of this superfamily in <i>Brassica napus</i> has not been reported. In this study, 158 <i>BnaBAHD</i> genes were identified by comprehensive analyses of evolutionary relationships, motif structures, chromosomal distribution, gene collinearity, and selection pressures, and these genes were phylogenetically classified into five clades harboring conserved catalytic domains (HXXXD and DFGWG). Transient overexpression combined with metabolomic profiling demonstrated that two homologous seed-specific Clade V members, <i>BnaBAHD040</i> and <i>BnaBAHD120</i>, which exhibited elevated expression during late seed development, significantly enhanced the accumulation of acylated metabolites contributing to biotic/abiotic stress resistance. This study provides the first experimental validation of the catalytic functions of BAHD enzymes in <i>B. napus</i>, establishing a theoretical foundation for leveraging this gene family in genetic improvement to develop novel rapeseed cultivars with enhanced stress tolerance and yield. |
| format | Article |
| id | doaj-art-cd2929ea2da04594b44ff74afb820994 |
| institution | DOAJ |
| issn | 2223-7747 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj-art-cd2929ea2da04594b44ff74afb8209942025-08-20T03:08:06ZengMDPI AGPlants2223-77472025-07-011414218310.3390/plants14142183Genome-Wide Identification and Functional Characterization of the BAHD Acyltransferase Gene Family in <i>Brassica napus</i> L.Yuanyuan Liu0Xingzhi Wei1Yiwei Liu2Yunshan Tang3Shulin Shen4Jie Xu5Lulu Chen6Cunmin Qu7Huiyan Zhao8Hai Du9Huafang Wan10Nengwen Yin11Ti Zhang12Integrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, ChinaIntegrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, ChinaIntegrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, ChinaIntegrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, ChinaIntegrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, ChinaIntegrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, ChinaIntegrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, ChinaIntegrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, ChinaIntegrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, ChinaIntegrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, ChinaIntegrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, ChinaIntegrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, ChinaIntegrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, ChinaThe BAHD acyltransferase family plays a critical role in plant secondary metabolism by catalyzing acyl transfer reactions that are essential for synthesizing metabolites involved in environmental adaptation. However, systematic investigation of this superfamily in <i>Brassica napus</i> has not been reported. In this study, 158 <i>BnaBAHD</i> genes were identified by comprehensive analyses of evolutionary relationships, motif structures, chromosomal distribution, gene collinearity, and selection pressures, and these genes were phylogenetically classified into five clades harboring conserved catalytic domains (HXXXD and DFGWG). Transient overexpression combined with metabolomic profiling demonstrated that two homologous seed-specific Clade V members, <i>BnaBAHD040</i> and <i>BnaBAHD120</i>, which exhibited elevated expression during late seed development, significantly enhanced the accumulation of acylated metabolites contributing to biotic/abiotic stress resistance. This study provides the first experimental validation of the catalytic functions of BAHD enzymes in <i>B. napus</i>, establishing a theoretical foundation for leveraging this gene family in genetic improvement to develop novel rapeseed cultivars with enhanced stress tolerance and yield.https://www.mdpi.com/2223-7747/14/14/2183BAHD acyltransferase<i>Brassica napus</i> L.acylationstress resistance |
| spellingShingle | Yuanyuan Liu Xingzhi Wei Yiwei Liu Yunshan Tang Shulin Shen Jie Xu Lulu Chen Cunmin Qu Huiyan Zhao Hai Du Huafang Wan Nengwen Yin Ti Zhang Genome-Wide Identification and Functional Characterization of the BAHD Acyltransferase Gene Family in <i>Brassica napus</i> L. Plants BAHD acyltransferase <i>Brassica napus</i> L. acylation stress resistance |
| title | Genome-Wide Identification and Functional Characterization of the BAHD Acyltransferase Gene Family in <i>Brassica napus</i> L. |
| title_full | Genome-Wide Identification and Functional Characterization of the BAHD Acyltransferase Gene Family in <i>Brassica napus</i> L. |
| title_fullStr | Genome-Wide Identification and Functional Characterization of the BAHD Acyltransferase Gene Family in <i>Brassica napus</i> L. |
| title_full_unstemmed | Genome-Wide Identification and Functional Characterization of the BAHD Acyltransferase Gene Family in <i>Brassica napus</i> L. |
| title_short | Genome-Wide Identification and Functional Characterization of the BAHD Acyltransferase Gene Family in <i>Brassica napus</i> L. |
| title_sort | genome wide identification and functional characterization of the bahd acyltransferase gene family in i brassica napus i l |
| topic | BAHD acyltransferase <i>Brassica napus</i> L. acylation stress resistance |
| url | https://www.mdpi.com/2223-7747/14/14/2183 |
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