ROS‐Driven Nanoventilator for MRSA‐Induced Acute Lung Injury Treatment via In Situ Oxygen Supply, Anti‐Inflammation and Immunomodulation

Abstract Hypoxia, inflammatory response and pathogen (bacterial or viral) infection are the three main factors that lead to death in patients with acute lung injury (ALI). Among them, hypoxia activates the expression of HIF‐1α, further exacerbating the production of ROS and inflammatory response. Cu...

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Main Authors: Zheng Luo, Qi Wang, Xiaotong Fan, Xue Qi Koh, Xian Jun Loh, Caisheng Wu, Zibiao Li, Yun‐Long Wu
Format: Article
Language:English
Published: Wiley 2025-05-01
Series:Advanced Science
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Online Access:https://doi.org/10.1002/advs.202406060
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author Zheng Luo
Qi Wang
Xiaotong Fan
Xue Qi Koh
Xian Jun Loh
Caisheng Wu
Zibiao Li
Yun‐Long Wu
author_facet Zheng Luo
Qi Wang
Xiaotong Fan
Xue Qi Koh
Xian Jun Loh
Caisheng Wu
Zibiao Li
Yun‐Long Wu
author_sort Zheng Luo
collection DOAJ
description Abstract Hypoxia, inflammatory response and pathogen (bacterial or viral) infection are the three main factors that lead to death in patients with acute lung injury (ALI). Among them, hypoxia activates the expression of HIF‐1α, further exacerbating the production of ROS and inflammatory response. Currently, anti‐inflammatory and pathogen elimination treatment strategies have effectively alleviated infectious pneumonia, but improving lung hypoxia still faces challenges. Here, a vancomycin‐loaded nanoventilator (SCVN) containing superoxide dismutase (SOD) and catalase (CAT) is developed, which is prepared by encapsulating SOD, CAT and vancomycin into a nanocapsule by in situ polymerization. This nanocapsule can effectively improve the stability and loading rate of enzymes, and enhance their enzyme cascade efficiency, thereby efficiently consuming •O2− and H2O2 to generate O2 in situ and reducing ROS level. More interestingly, in situ O2 supply can effectively relieve lung hypoxia to reduce HIF‐1α expression and balance the number of M1/M2 macrophages to reduce the levels of TNF‐α, IL‐1β and IL‐6, thereby alleviating the inflammatory response. Meanwhile, vancomycin can target and kill MRSA, fundamentally solving the cause of pneumonia. This nanoventilator with antibacterial, anti‐inflammatory, ROS scavenging and in situ O2 supply functions will provide a universal clinical treatment strategy for ALI caused by pathogens.
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spelling doaj-art-cd24eba0e35648649e33de5dbeece5f02025-08-20T02:08:14ZengWileyAdvanced Science2198-38442025-05-011218n/an/a10.1002/advs.202406060ROS‐Driven Nanoventilator for MRSA‐Induced Acute Lung Injury Treatment via In Situ Oxygen Supply, Anti‐Inflammation and ImmunomodulationZheng Luo0Qi Wang1Xiaotong Fan2Xue Qi Koh3Xian Jun Loh4Caisheng Wu5Zibiao Li6Yun‐Long Wu7State Key Laboratory of Cellular Stress Biology Fujian Provincial Key Laboratory of Innovative Drug Target Research School of Pharmaceutical Sciences Xiamen University Xiamen 361102 ChinaState Key Laboratory of Cellular Stress Biology Fujian Provincial Key Laboratory of Innovative Drug Target Research School of Pharmaceutical Sciences Xiamen University Xiamen 361102 ChinaInstitute of Sustainability for Chemicals Energy and Environment (ISCE2) Agency for Science, Technology and Research (A*STAR) 1 Pesek Road, Jurong Island Singapore 627833 Republic of SingaporeInstitute of Materials Research and Engineering (IMRE) Agency for Science Technology and Research (A*STAR) 2 Fusionopolis Way, Innovis #08‐03 Singapore 138634 Republic of SingaporeInstitute of Materials Research and Engineering (IMRE) Agency for Science Technology and Research (A*STAR) 2 Fusionopolis Way, Innovis #08‐03 Singapore 138634 Republic of SingaporeState Key Laboratory of Cellular Stress Biology Fujian Provincial Key Laboratory of Innovative Drug Target Research School of Pharmaceutical Sciences Xiamen University Xiamen 361102 ChinaInstitute of Materials Research and Engineering (IMRE) Agency for Science Technology and Research (A*STAR) 2 Fusionopolis Way, Innovis #08‐03 Singapore 138634 Republic of SingaporeState Key Laboratory of Cellular Stress Biology Fujian Provincial Key Laboratory of Innovative Drug Target Research School of Pharmaceutical Sciences Xiamen University Xiamen 361102 ChinaAbstract Hypoxia, inflammatory response and pathogen (bacterial or viral) infection are the three main factors that lead to death in patients with acute lung injury (ALI). Among them, hypoxia activates the expression of HIF‐1α, further exacerbating the production of ROS and inflammatory response. Currently, anti‐inflammatory and pathogen elimination treatment strategies have effectively alleviated infectious pneumonia, but improving lung hypoxia still faces challenges. Here, a vancomycin‐loaded nanoventilator (SCVN) containing superoxide dismutase (SOD) and catalase (CAT) is developed, which is prepared by encapsulating SOD, CAT and vancomycin into a nanocapsule by in situ polymerization. This nanocapsule can effectively improve the stability and loading rate of enzymes, and enhance their enzyme cascade efficiency, thereby efficiently consuming •O2− and H2O2 to generate O2 in situ and reducing ROS level. More interestingly, in situ O2 supply can effectively relieve lung hypoxia to reduce HIF‐1α expression and balance the number of M1/M2 macrophages to reduce the levels of TNF‐α, IL‐1β and IL‐6, thereby alleviating the inflammatory response. Meanwhile, vancomycin can target and kill MRSA, fundamentally solving the cause of pneumonia. This nanoventilator with antibacterial, anti‐inflammatory, ROS scavenging and in situ O2 supply functions will provide a universal clinical treatment strategy for ALI caused by pathogens.https://doi.org/10.1002/advs.202406060acute lung injuryanti‐inflammatoryenzyme therapyoxygen supplyROS clearance
spellingShingle Zheng Luo
Qi Wang
Xiaotong Fan
Xue Qi Koh
Xian Jun Loh
Caisheng Wu
Zibiao Li
Yun‐Long Wu
ROS‐Driven Nanoventilator for MRSA‐Induced Acute Lung Injury Treatment via In Situ Oxygen Supply, Anti‐Inflammation and Immunomodulation
Advanced Science
acute lung injury
anti‐inflammatory
enzyme therapy
oxygen supply
ROS clearance
title ROS‐Driven Nanoventilator for MRSA‐Induced Acute Lung Injury Treatment via In Situ Oxygen Supply, Anti‐Inflammation and Immunomodulation
title_full ROS‐Driven Nanoventilator for MRSA‐Induced Acute Lung Injury Treatment via In Situ Oxygen Supply, Anti‐Inflammation and Immunomodulation
title_fullStr ROS‐Driven Nanoventilator for MRSA‐Induced Acute Lung Injury Treatment via In Situ Oxygen Supply, Anti‐Inflammation and Immunomodulation
title_full_unstemmed ROS‐Driven Nanoventilator for MRSA‐Induced Acute Lung Injury Treatment via In Situ Oxygen Supply, Anti‐Inflammation and Immunomodulation
title_short ROS‐Driven Nanoventilator for MRSA‐Induced Acute Lung Injury Treatment via In Situ Oxygen Supply, Anti‐Inflammation and Immunomodulation
title_sort ros driven nanoventilator for mrsa induced acute lung injury treatment via in situ oxygen supply anti inflammation and immunomodulation
topic acute lung injury
anti‐inflammatory
enzyme therapy
oxygen supply
ROS clearance
url https://doi.org/10.1002/advs.202406060
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