Influence of Secondary Hyperparathyroidism Induced by Low Dietary Calcium, Vitamin D Deficiency, and Renal Failure on Circulating Rat PTH Molecular Forms
Rats(r) with secondary hyperparathyroidism were studied to define the relationship between vitamin D metabolites and rPTH levels measured by 3 different rat ELISAs. Controls and renal failure (RF) rats were on a normal diet, while 2 groups on a low-calcium (-Ca)...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2011-01-01
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| Series: | International Journal of Endocrinology |
| Online Access: | http://dx.doi.org/10.1155/2011/469783 |
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| Summary: | Rats(r) with secondary hyperparathyroidism were studied to define the relationship between vitamin
D metabolites and rPTH levels measured by 3 different rat ELISAs. Controls and renal failure (RF) rats were on
a normal diet, while 2 groups on a low-calcium (-Ca) or a vitamin D-deficient (-D) diet. RF was induced surgically. Mild RF rats
had normal calcium and 25(OH)D but reduced 1,25(OH)2D levels (P<.001) with a 2.5-fold increased in rPTH (P<.001). Severe RF rats and those on a -Ca or -D diet had reduced calcium (P<.01) and 25(OH)D levels (P<.05), with rPTH increased by 2 (-Ca diet; P<.05), 4 (-D diet; P<.001), and 20-folds (RF; P<.001) while 1,25(OH)2D was high (-Ca diet: P<.001) or low (-D diet, RF: P<.001). 25(OH)D and 1,25(OH)2D were positively and negatively related on the -Ca and -D diets, respectively.
rPTH molecular forms behaved as expected in RF and on -Ca diet, but not on -D diet with more C-rPTH fragments
when less were expected. This may be related to the short-time course of this study compared to prior studies. |
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| ISSN: | 1687-8337 1687-8345 |