EXPRESSION OF SELECTED miRNAs IN CIRCULATING BLOOD OF EARLY AND LATE-ONSET ALZHEIMER DISEASE PATIENTS
Objective: Inflammation and associated microRNAs (miRNA) both play essential roles in the pathogenesis of Alzheimer’s disease (AD). The expression profile of miRNA’s in an AD brain, also reflected in the peripheral blood mononuclear cells, may give support to the inflammatory changes seen in the cou...
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Istanbul University Press
2021-04-01
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| Series: | İstanbul Tıp Fakültesi Dergisi |
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| Online Access: | https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/017B456E5DCD4FEC95613D37D65EFDA0 |
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| author | Gamze Güven Ebba Lohmann Çağrı Güleç Başar Bilgiç Ebru Özer Haşmet Ayhan Hanağası İbrahim Hakan Gürvit Nihan Erginel Ünaltuna |
| author_facet | Gamze Güven Ebba Lohmann Çağrı Güleç Başar Bilgiç Ebru Özer Haşmet Ayhan Hanağası İbrahim Hakan Gürvit Nihan Erginel Ünaltuna |
| author_sort | Gamze Güven |
| collection | DOAJ |
| description | Objective: Inflammation and associated microRNAs (miRNA) both play essential roles in the pathogenesis of Alzheimer’s disease (AD). The expression profile of miRNA’s in an AD brain, also reflected in the peripheral blood mononuclear cells, may give support to the inflammatory changes seen in the course of AD. We aimed to investigate the expression levels of specific inflammatory miRNAs (mir-146a, mir-144, mir-34a) in both blood leukocytes and plasma of AD patients and to evaluate their potential usability as biomarkers in AD diagnosis and to also demonstrate whether the expression of these miRNAs differ between early and late-onset AD patients. Methods: We investigated the expression levels of miRNAs in 16 early-onset and 26 late-onset AD patients and in their respective controls by using qRT-PCR. Results: Plasma mir-144 levels were significantly different between EOAD and LOAD patients (p=0.015). In addition, levels of leukocyte mir-34a were significantly down-regulated in EOAD compared to LOAD patients (p=0.027). Our results also showed significant positive correlations between age and plasma mir144 and leukocyte mir-34a expressions. Conclusion: The differential expression of plasma mir-144 and leukocyte mir-34a between EOAD and LOAD patients might reflect age-dependent changes in miRNA expression and might be independent of the disease status. |
| format | Article |
| id | doaj-art-cd1ce3edfb1d435fa6f2df703e8f5056 |
| institution | OA Journals |
| issn | 1305-6441 |
| language | English |
| publishDate | 2021-04-01 |
| publisher | Istanbul University Press |
| record_format | Article |
| series | İstanbul Tıp Fakültesi Dergisi |
| spelling | doaj-art-cd1ce3edfb1d435fa6f2df703e8f50562025-08-20T02:15:33ZengIstanbul University Pressİstanbul Tıp Fakültesi Dergisi1305-64412021-04-0184216517410.26650/IUITFD.2020.0061123456EXPRESSION OF SELECTED miRNAs IN CIRCULATING BLOOD OF EARLY AND LATE-ONSET ALZHEIMER DISEASE PATIENTSGamze Güven0https://orcid.org/0000-0001-8576-5843Ebba Lohmann1https://orcid.org/0000-0001-8695-7919Çağrı Güleç2https://orcid.org/0000-0002-1256-9574Başar Bilgiç3https://orcid.org/0000-0001-6032-0856Ebru Özer4https://orcid.org/0000-0002-7092-7624Haşmet Ayhan Hanağası5https://orcid.org/0000-0001-9645-7707İbrahim Hakan Gürvit6https://orcid.org/0000-0003-2908-8475Nihan Erginel Ünaltuna7https://orcid.org/0000-0003-0562-0455İstanbul Üniversitesi, İstanbul, TürkiyeTübingen University, Tübingen, Germanyİstanbul Üniversitesi, İstanbul, Türkiyeİstanbul Üniversitesi, İstanbul, Türkiyeİstanbul Üniversitesi, İstanbul, Türkiyeİstanbul Üniversitesi, İstanbul, Türkiyeİstanbul Üniversitesi, İstanbul, Türkiyeİstanbul Üniversitesi, İstanbul, TürkiyeObjective: Inflammation and associated microRNAs (miRNA) both play essential roles in the pathogenesis of Alzheimer’s disease (AD). The expression profile of miRNA’s in an AD brain, also reflected in the peripheral blood mononuclear cells, may give support to the inflammatory changes seen in the course of AD. We aimed to investigate the expression levels of specific inflammatory miRNAs (mir-146a, mir-144, mir-34a) in both blood leukocytes and plasma of AD patients and to evaluate their potential usability as biomarkers in AD diagnosis and to also demonstrate whether the expression of these miRNAs differ between early and late-onset AD patients. Methods: We investigated the expression levels of miRNAs in 16 early-onset and 26 late-onset AD patients and in their respective controls by using qRT-PCR. Results: Plasma mir-144 levels were significantly different between EOAD and LOAD patients (p=0.015). In addition, levels of leukocyte mir-34a were significantly down-regulated in EOAD compared to LOAD patients (p=0.027). Our results also showed significant positive correlations between age and plasma mir144 and leukocyte mir-34a expressions. Conclusion: The differential expression of plasma mir-144 and leukocyte mir-34a between EOAD and LOAD patients might reflect age-dependent changes in miRNA expression and might be independent of the disease status.https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/017B456E5DCD4FEC95613D37D65EFDA0alzheimer diseasemirnamirna expression |
| spellingShingle | Gamze Güven Ebba Lohmann Çağrı Güleç Başar Bilgiç Ebru Özer Haşmet Ayhan Hanağası İbrahim Hakan Gürvit Nihan Erginel Ünaltuna EXPRESSION OF SELECTED miRNAs IN CIRCULATING BLOOD OF EARLY AND LATE-ONSET ALZHEIMER DISEASE PATIENTS İstanbul Tıp Fakültesi Dergisi alzheimer disease mirna mirna expression |
| title | EXPRESSION OF SELECTED miRNAs IN CIRCULATING BLOOD OF EARLY AND LATE-ONSET ALZHEIMER DISEASE PATIENTS |
| title_full | EXPRESSION OF SELECTED miRNAs IN CIRCULATING BLOOD OF EARLY AND LATE-ONSET ALZHEIMER DISEASE PATIENTS |
| title_fullStr | EXPRESSION OF SELECTED miRNAs IN CIRCULATING BLOOD OF EARLY AND LATE-ONSET ALZHEIMER DISEASE PATIENTS |
| title_full_unstemmed | EXPRESSION OF SELECTED miRNAs IN CIRCULATING BLOOD OF EARLY AND LATE-ONSET ALZHEIMER DISEASE PATIENTS |
| title_short | EXPRESSION OF SELECTED miRNAs IN CIRCULATING BLOOD OF EARLY AND LATE-ONSET ALZHEIMER DISEASE PATIENTS |
| title_sort | expression of selected mirnas in circulating blood of early and late onset alzheimer disease patients |
| topic | alzheimer disease mirna mirna expression |
| url | https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/017B456E5DCD4FEC95613D37D65EFDA0 |
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