Diagnostic Reliability of Methylene Blue in Vivo Staining in Predicting Oral Premalignant and Malignant Lesions with Scrape Cytology and Histopathological Correlation
Background and aim: To correlate the clinical diagnosis of the suspected lesions of oral cavity using methylene blue stain, histopathological and cytopathological methods.Material and Methods: The study has been carried out in the Pathology department for a period of 1.5 years. The study includes in...
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International Journal of Scientific Research in Dental and Medical Sciences (IJSRDMS)
2024-10-01
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| author | Mayuri Bapodra Alpeshkumar Chavda Vibhooti Dhum Pravina Santwani |
| author_facet | Mayuri Bapodra Alpeshkumar Chavda Vibhooti Dhum Pravina Santwani |
| author_sort | Mayuri Bapodra |
| collection | DOAJ |
| description | Background and aim: To correlate the clinical diagnosis of the suspected lesions of oral cavity using methylene blue stain, histopathological and cytopathological methods.Material and Methods: The study has been carried out in the Pathology department for a period of 1.5 years. The study includes in vivo application of methylene blue stain to clinically suspected premalignant and malignant lesions of oral cavity, followed by scrape cytology from the same site followed by its histopathological examination among 100 subjects which includes 60 cases and 40 controls. Scrape smear was collected from all the 100 subjects. Biopsy was done in 48 cases out of 60 cases.Results: Out of 48 cases, maximum lesions were found to be malignant (71%), followed by premalignant (17%) oral lesions. Benign lesions were found in male patients only and malignant lesions (80%) and premalignant lesions (75%) were found in majority of males. Majority of patients (70%) had Well differentiated Squamous cell carcinoma, followed by 24% of patients having Moderately differentiated Squamous cell carcinoma.Conclusions: The current study shows that majority of patients presented with carcinoma and very few at premalignant stage. There is prevalence of habits of tobacco chewing/smoking betel nut in India which are carcinogenic. So, the present study focused on highlighting the importance of screening of oral lesions to diagnose the oral lesions at early stage for its timely treatment and to improve the quality of life. In vivo methylene blue staining is simple, easy to perform, noninvasive, rapid, painless, inexpensive method. |
| format | Article |
| id | doaj-art-cd1601b108e84baba7c6022abf57976d |
| institution | DOAJ |
| issn | 2676-5497 2676-5373 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | International Journal of Scientific Research in Dental and Medical Sciences (IJSRDMS) |
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| series | International Journal of Scientific Research in Dental and Medical Sciences |
| spelling | doaj-art-cd1601b108e84baba7c6022abf57976d2025-08-20T03:00:57ZengInternational Journal of Scientific Research in Dental and Medical Sciences (IJSRDMS)International Journal of Scientific Research in Dental and Medical Sciences2676-54972676-53732024-10-016416316710.30485/ijsrdms.2024.473345.1604214461Diagnostic Reliability of Methylene Blue in Vivo Staining in Predicting Oral Premalignant and Malignant Lesions with Scrape Cytology and Histopathological CorrelationMayuri Bapodra0Alpeshkumar Chavda1Vibhooti Dhum2Pravina Santwani3Department of Pathology, GMERS Medical College, Porbandar, Bhakta Kavi Narsinh Mehta University, Gujarat, IndiaDepartment of Pathology, GMERS Medical College, Porbandar, Bhakta Kavi Narsinh Mehta University, Gujarat, IndiaDepartment of Pathology, GMERS Medical College, Porbandar, Bhakta Kavi Narsinh Mehta University, Gujarat, IndiaDepartment of Pathology, Shantabaa Medical College and General Hospital Amreli, Amreli, IndiaBackground and aim: To correlate the clinical diagnosis of the suspected lesions of oral cavity using methylene blue stain, histopathological and cytopathological methods.Material and Methods: The study has been carried out in the Pathology department for a period of 1.5 years. The study includes in vivo application of methylene blue stain to clinically suspected premalignant and malignant lesions of oral cavity, followed by scrape cytology from the same site followed by its histopathological examination among 100 subjects which includes 60 cases and 40 controls. Scrape smear was collected from all the 100 subjects. Biopsy was done in 48 cases out of 60 cases.Results: Out of 48 cases, maximum lesions were found to be malignant (71%), followed by premalignant (17%) oral lesions. Benign lesions were found in male patients only and malignant lesions (80%) and premalignant lesions (75%) were found in majority of males. Majority of patients (70%) had Well differentiated Squamous cell carcinoma, followed by 24% of patients having Moderately differentiated Squamous cell carcinoma.Conclusions: The current study shows that majority of patients presented with carcinoma and very few at premalignant stage. There is prevalence of habits of tobacco chewing/smoking betel nut in India which are carcinogenic. So, the present study focused on highlighting the importance of screening of oral lesions to diagnose the oral lesions at early stage for its timely treatment and to improve the quality of life. In vivo methylene blue staining is simple, easy to perform, noninvasive, rapid, painless, inexpensive method.http://www.ijsrdms.com/article_214461_7dd83e4e6d61ea426ae8f6b78e751b86.pdfcarcinomacytologymethylene blue |
| spellingShingle | Mayuri Bapodra Alpeshkumar Chavda Vibhooti Dhum Pravina Santwani Diagnostic Reliability of Methylene Blue in Vivo Staining in Predicting Oral Premalignant and Malignant Lesions with Scrape Cytology and Histopathological Correlation International Journal of Scientific Research in Dental and Medical Sciences carcinoma cytology methylene blue |
| title | Diagnostic Reliability of Methylene Blue in Vivo Staining in Predicting Oral Premalignant and Malignant Lesions with Scrape Cytology and Histopathological Correlation |
| title_full | Diagnostic Reliability of Methylene Blue in Vivo Staining in Predicting Oral Premalignant and Malignant Lesions with Scrape Cytology and Histopathological Correlation |
| title_fullStr | Diagnostic Reliability of Methylene Blue in Vivo Staining in Predicting Oral Premalignant and Malignant Lesions with Scrape Cytology and Histopathological Correlation |
| title_full_unstemmed | Diagnostic Reliability of Methylene Blue in Vivo Staining in Predicting Oral Premalignant and Malignant Lesions with Scrape Cytology and Histopathological Correlation |
| title_short | Diagnostic Reliability of Methylene Blue in Vivo Staining in Predicting Oral Premalignant and Malignant Lesions with Scrape Cytology and Histopathological Correlation |
| title_sort | diagnostic reliability of methylene blue in vivo staining in predicting oral premalignant and malignant lesions with scrape cytology and histopathological correlation |
| topic | carcinoma cytology methylene blue |
| url | http://www.ijsrdms.com/article_214461_7dd83e4e6d61ea426ae8f6b78e751b86.pdf |
| work_keys_str_mv | AT mayuribapodra diagnosticreliabilityofmethyleneblueinvivostaininginpredictingoralpremalignantandmalignantlesionswithscrapecytologyandhistopathologicalcorrelation AT alpeshkumarchavda diagnosticreliabilityofmethyleneblueinvivostaininginpredictingoralpremalignantandmalignantlesionswithscrapecytologyandhistopathologicalcorrelation AT vibhootidhum diagnosticreliabilityofmethyleneblueinvivostaininginpredictingoralpremalignantandmalignantlesionswithscrapecytologyandhistopathologicalcorrelation AT pravinasantwani diagnosticreliabilityofmethyleneblueinvivostaininginpredictingoralpremalignantandmalignantlesionswithscrapecytologyandhistopathologicalcorrelation |