Isolation, Identification, and Molecular Genetic Characteristics of a Pathogenic Strain of <i>Streptococcus suis</i> Serotype 3
<i>Streptococcus suis</i> (<i>S. suis</i>) is considered as one of the most crucial bacterial pathogens that leads to serious economic losses to the swine industry. Different <i>S. suis</i> serotypes exhibit diverse characteristics in population structure and path...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-02-01
|
| Series: | Pathogens |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-0817/14/2/192 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849719465132949504 |
|---|---|
| author | Longbai Wang Jingli Qiu Bing He Xuemin Wu Qiuyong Chen Quanxi Wang Renjie Wu Bohan Zheng Lunjiang Zhou Xiaohong Huang |
| author_facet | Longbai Wang Jingli Qiu Bing He Xuemin Wu Qiuyong Chen Quanxi Wang Renjie Wu Bohan Zheng Lunjiang Zhou Xiaohong Huang |
| author_sort | Longbai Wang |
| collection | DOAJ |
| description | <i>Streptococcus suis</i> (<i>S. suis</i>) is considered as one of the most crucial bacterial pathogens that leads to serious economic losses to the swine industry. Different <i>S. suis</i> serotypes exhibit diverse characteristics in population structure and pathogenicity. Epidemiology data underscore the importance of <i>S. suis</i> serotype 3 (SS3). However, except for a few epidemiological information, limited study information is available on this serotype. Herein, a pathogenic SS3 (the <i>S. suis</i> strain YA) was isolated from infected piglets in clinical practice, and then whole genome sequencing and analysis, hemolytic activity, antimicrobial susceptibility, pathogenicity to mice and piglets were conducted. The results of the whole genome sequencing of the <i>S. suis</i> strain YA showed that the complete genome was 2,167,682 bp in length with a G + C content of 41.2% and exhibited a unique sequence type (ST1801). The result of phylogenetic tree showed that it was most closely related to strain DNC15 and 6407 (ST54) from Denmark. The <i>tet(W)</i> and <i>erm(B)</i> resistant genes were identified in the <i>S. suis</i> strain YA by inserting into rum locus, in accordance with the result of resistance to tetracyclines and macrolide-lincosamide-streptogramin antibiotics. Twenty-seven key virulence factors were detected in the <i>S. suis</i> strain YA, including <i>sly</i>, <i>ef</i> and <i>mrp</i>, which contribute to pathogenicity in mice and piglets, causing bleeding and congestion in multiple tissue organs especially in the brains. And the LD<sub>50</sub> value for mice was 1.54 × 10<sup>7</sup> CFU. Therefore, our research emphasizes the importance of understanding SS3, and provides valuable information for the scientific prevention and control of <i>S. suis</i>. |
| format | Article |
| id | doaj-art-cd11a1537acf4e2f8df48085f6e09981 |
| institution | DOAJ |
| issn | 2076-0817 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj-art-cd11a1537acf4e2f8df48085f6e099812025-08-20T03:12:09ZengMDPI AGPathogens2076-08172025-02-0114219210.3390/pathogens14020192Isolation, Identification, and Molecular Genetic Characteristics of a Pathogenic Strain of <i>Streptococcus suis</i> Serotype 3Longbai Wang0Jingli Qiu1Bing He2Xuemin Wu3Qiuyong Chen4Quanxi Wang5Renjie Wu6Bohan Zheng7Lunjiang Zhou8Xiaohong Huang9Fujian Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health, College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, ChinaFujian Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health, College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, ChinaFujian Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health, College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou 350013, ChinaFujian Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health, College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China<i>Streptococcus suis</i> (<i>S. suis</i>) is considered as one of the most crucial bacterial pathogens that leads to serious economic losses to the swine industry. Different <i>S. suis</i> serotypes exhibit diverse characteristics in population structure and pathogenicity. Epidemiology data underscore the importance of <i>S. suis</i> serotype 3 (SS3). However, except for a few epidemiological information, limited study information is available on this serotype. Herein, a pathogenic SS3 (the <i>S. suis</i> strain YA) was isolated from infected piglets in clinical practice, and then whole genome sequencing and analysis, hemolytic activity, antimicrobial susceptibility, pathogenicity to mice and piglets were conducted. The results of the whole genome sequencing of the <i>S. suis</i> strain YA showed that the complete genome was 2,167,682 bp in length with a G + C content of 41.2% and exhibited a unique sequence type (ST1801). The result of phylogenetic tree showed that it was most closely related to strain DNC15 and 6407 (ST54) from Denmark. The <i>tet(W)</i> and <i>erm(B)</i> resistant genes were identified in the <i>S. suis</i> strain YA by inserting into rum locus, in accordance with the result of resistance to tetracyclines and macrolide-lincosamide-streptogramin antibiotics. Twenty-seven key virulence factors were detected in the <i>S. suis</i> strain YA, including <i>sly</i>, <i>ef</i> and <i>mrp</i>, which contribute to pathogenicity in mice and piglets, causing bleeding and congestion in multiple tissue organs especially in the brains. And the LD<sub>50</sub> value for mice was 1.54 × 10<sup>7</sup> CFU. Therefore, our research emphasizes the importance of understanding SS3, and provides valuable information for the scientific prevention and control of <i>S. suis</i>.https://www.mdpi.com/2076-0817/14/2/192<i>Streptococcus suis</i> serotype 3virulence factorspathogenicsequence analysis |
| spellingShingle | Longbai Wang Jingli Qiu Bing He Xuemin Wu Qiuyong Chen Quanxi Wang Renjie Wu Bohan Zheng Lunjiang Zhou Xiaohong Huang Isolation, Identification, and Molecular Genetic Characteristics of a Pathogenic Strain of <i>Streptococcus suis</i> Serotype 3 Pathogens <i>Streptococcus suis</i> serotype 3 virulence factors pathogenic sequence analysis |
| title | Isolation, Identification, and Molecular Genetic Characteristics of a Pathogenic Strain of <i>Streptococcus suis</i> Serotype 3 |
| title_full | Isolation, Identification, and Molecular Genetic Characteristics of a Pathogenic Strain of <i>Streptococcus suis</i> Serotype 3 |
| title_fullStr | Isolation, Identification, and Molecular Genetic Characteristics of a Pathogenic Strain of <i>Streptococcus suis</i> Serotype 3 |
| title_full_unstemmed | Isolation, Identification, and Molecular Genetic Characteristics of a Pathogenic Strain of <i>Streptococcus suis</i> Serotype 3 |
| title_short | Isolation, Identification, and Molecular Genetic Characteristics of a Pathogenic Strain of <i>Streptococcus suis</i> Serotype 3 |
| title_sort | isolation identification and molecular genetic characteristics of a pathogenic strain of i streptococcus suis i serotype 3 |
| topic | <i>Streptococcus suis</i> serotype 3 virulence factors pathogenic sequence analysis |
| url | https://www.mdpi.com/2076-0817/14/2/192 |
| work_keys_str_mv | AT longbaiwang isolationidentificationandmoleculargeneticcharacteristicsofapathogenicstrainofistreptococcussuisiserotype3 AT jingliqiu isolationidentificationandmoleculargeneticcharacteristicsofapathogenicstrainofistreptococcussuisiserotype3 AT binghe isolationidentificationandmoleculargeneticcharacteristicsofapathogenicstrainofistreptococcussuisiserotype3 AT xueminwu isolationidentificationandmoleculargeneticcharacteristicsofapathogenicstrainofistreptococcussuisiserotype3 AT qiuyongchen isolationidentificationandmoleculargeneticcharacteristicsofapathogenicstrainofistreptococcussuisiserotype3 AT quanxiwang isolationidentificationandmoleculargeneticcharacteristicsofapathogenicstrainofistreptococcussuisiserotype3 AT renjiewu isolationidentificationandmoleculargeneticcharacteristicsofapathogenicstrainofistreptococcussuisiserotype3 AT bohanzheng isolationidentificationandmoleculargeneticcharacteristicsofapathogenicstrainofistreptococcussuisiserotype3 AT lunjiangzhou isolationidentificationandmoleculargeneticcharacteristicsofapathogenicstrainofistreptococcussuisiserotype3 AT xiaohonghuang isolationidentificationandmoleculargeneticcharacteristicsofapathogenicstrainofistreptococcussuisiserotype3 |