mTOR-related linc-PMB promotes mitochondrial biogenesis via stabilizing SIRT1 mRNA through binding to the HuR protein
Mitochondrial dysfunction is implicated in numerous disorders, including type 2 diabetes, Alzheimer’s disease and cancer. Long non-coding RNAs (lncRNAs) are emerging as pivotal regulators of cellular energy metabolism, yet their roles remai...
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| Format: | Article |
| Language: | English |
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China Science Publishing & Media Ltd.
2025-01-01
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| Series: | Acta Biochimica et Biophysica Sinica |
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| Online Access: | https://www.sciengine.com/doi/10.3724/abbs.2024236 |
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| author | Chen Qian Zhang Huaying Wang Daokun Liao Wenjing Liu Yazhou Cai Yurui Wang Siyou Yu Mengqian |
| author_facet | Chen Qian Zhang Huaying Wang Daokun Liao Wenjing Liu Yazhou Cai Yurui Wang Siyou Yu Mengqian |
| author_sort | Chen Qian |
| collection | DOAJ |
| description | Mitochondrial dysfunction is implicated in numerous disorders, including type 2 diabetes, Alzheimer’s disease and cancer. Long non-coding RNAs (lncRNAs) are emerging as pivotal regulators of cellular energy metabolism, yet their roles remain largely unclear. In this study, we identify an lncRNA named linc-PMB, which is associated with mTOR and promotes mitochondrial biogenesis, through microarray analysis. We demonstrate that the knockdown of linc-PMB results in significantly impaired mitochondrial respiration and biogenesis, along with altered expressions of related genes. Conversely, overexpression of linc-PMB markedly increases mitochondrial function. We further reveal that linc-PMB interacts with the RNA-binding protein HuR, promoting the stabilization of SIRT1 mRNA and a substantial increase in SIRT1 expression, which in turn activates the PGC-1α/mtTFA pathway and mitochondrial biogenesis. Collectively, our findings reveal a novel regulatory pathway in which linc-PMB, through its interaction with HuR, modulates the SIRT1/PGC-1α/mtTFA axis to maintain mitochondrial biogenesis and function. |
| format | Article |
| id | doaj-art-cd0ecb78e10a42f4b691d4bbde9734eb |
| institution | Kabale University |
| issn | 1672-9145 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | China Science Publishing & Media Ltd. |
| record_format | Article |
| series | Acta Biochimica et Biophysica Sinica |
| spelling | doaj-art-cd0ecb78e10a42f4b691d4bbde9734eb2025-08-20T03:59:35ZengChina Science Publishing & Media Ltd.Acta Biochimica et Biophysica Sinica1672-91452025-01-01571057106710.3724/abbs.202423620d259ccmTOR-related linc-PMB promotes mitochondrial biogenesis via stabilizing SIRT1 mRNA through binding to the HuR proteinChen Qian0Zhang Huaying1Wang Daokun2Liao Wenjing3Liu Yazhou4Cai Yurui5Wang Siyou6Yu Mengqian7["Department of Laboratory Medicine, Chengdu Second People’s Hospital, Chengdu 610017, China"]["Department of Clinical Laboratory, Hangzhou Traditional Chinese Medicine Hospital Affiliated to Zhejiang Chinese Medical University, Hangzhou 310000, China"]["Department of Laboratory Medicine, Chengdu Second People’s Hospital, Chengdu 610017, China"]["Department of Laboratory Medicine, Chengdu Second People’s Hospital, Chengdu 610017, China"]["Department of Laboratory Medicine, Chengdu Second People’s Hospital, Chengdu 610017, China"]["Department of Laboratory Medicine, Chengdu Second People’s Hospital, Chengdu 610017, China"]["Department of Laboratory Medicine, Chengdu Second People’s Hospital, Chengdu 610017, China"]["Zhejiang Provincial Key Laboratory of Precision Diagnosis and Therapy for Major Gynecological Diseases, Women’s Hospital and Institute of Translational Medicine, Zhejiang University School of Medicine, Hangzhou 310000, China"]Mitochondrial dysfunction is implicated in numerous disorders, including type 2 diabetes, Alzheimer’s disease and cancer. Long non-coding RNAs (lncRNAs) are emerging as pivotal regulators of cellular energy metabolism, yet their roles remain largely unclear. In this study, we identify an lncRNA named linc-PMB, which is associated with mTOR and promotes mitochondrial biogenesis, through microarray analysis. We demonstrate that the knockdown of linc-PMB results in significantly impaired mitochondrial respiration and biogenesis, along with altered expressions of related genes. Conversely, overexpression of linc-PMB markedly increases mitochondrial function. We further reveal that linc-PMB interacts with the RNA-binding protein HuR, promoting the stabilization of SIRT1 mRNA and a substantial increase in SIRT1 expression, which in turn activates the PGC-1α/mtTFA pathway and mitochondrial biogenesis. Collectively, our findings reveal a novel regulatory pathway in which linc-PMB, through its interaction with HuR, modulates the SIRT1/PGC-1α/mtTFA axis to maintain mitochondrial biogenesis and function.https://www.sciengine.com/doi/10.3724/abbs.2024236HuRlong non-coding RNAmitochondrial biogenesismTORSIRT1 |
| spellingShingle | Chen Qian Zhang Huaying Wang Daokun Liao Wenjing Liu Yazhou Cai Yurui Wang Siyou Yu Mengqian mTOR-related linc-PMB promotes mitochondrial biogenesis via stabilizing SIRT1 mRNA through binding to the HuR protein Acta Biochimica et Biophysica Sinica HuR long non-coding RNA mitochondrial biogenesis mTOR SIRT1 |
| title | mTOR-related linc-PMB promotes mitochondrial biogenesis via stabilizing SIRT1 mRNA through binding to the HuR protein |
| title_full | mTOR-related linc-PMB promotes mitochondrial biogenesis via stabilizing SIRT1 mRNA through binding to the HuR protein |
| title_fullStr | mTOR-related linc-PMB promotes mitochondrial biogenesis via stabilizing SIRT1 mRNA through binding to the HuR protein |
| title_full_unstemmed | mTOR-related linc-PMB promotes mitochondrial biogenesis via stabilizing SIRT1 mRNA through binding to the HuR protein |
| title_short | mTOR-related linc-PMB promotes mitochondrial biogenesis via stabilizing SIRT1 mRNA through binding to the HuR protein |
| title_sort | mtor related linc pmb promotes mitochondrial biogenesis via stabilizing sirt1 mrna through binding to the hur protein |
| topic | HuR long non-coding RNA mitochondrial biogenesis mTOR SIRT1 |
| url | https://www.sciengine.com/doi/10.3724/abbs.2024236 |
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