Specificity of Methylation Assays in Cancer Research: A Guideline for Designing Primers and Probes
DNA methylation is an epigenetic regulation mechanism of genomic function, and aberrant methylation pattern has been found to be a common event in many diseases and human cancers. A large number of cancer studies have been focused on identification of methylation changes as biomarkers (i.e., breast...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | Obstetrics and Gynecology International |
| Online Access: | http://dx.doi.org/10.1155/2010/870865 |
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| _version_ | 1849696192376602624 |
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| author | Zeinab Barekati Ramin Radpour Corina Kohler Xiao Yan Zhong |
| author_facet | Zeinab Barekati Ramin Radpour Corina Kohler Xiao Yan Zhong |
| author_sort | Zeinab Barekati |
| collection | DOAJ |
| description | DNA methylation is an epigenetic regulation mechanism of genomic function, and aberrant methylation pattern has been found to be a common event in many diseases and human cancers. A large number of cancer studies have been focused on identification of methylation changes as biomarkers (i.e., breast cancer). However, still clinical use of them is very limited because of lack of specificity and sensitivity for diagnostic test. This highlights the critical need for specific primer and probe design to avoid false-positive detection of methylation profiling. The guideline and online web tools that are introduced in this paper might help to perform a successful experiment and to develop specific diagnosis biomarkers by designing right primer pair and probe prior to experimental step. |
| format | Article |
| id | doaj-art-cd0e68ef37ff42778a6f86aa507489c1 |
| institution | DOAJ |
| issn | 1687-9589 1687-9597 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Obstetrics and Gynecology International |
| spelling | doaj-art-cd0e68ef37ff42778a6f86aa507489c12025-08-20T03:19:32ZengWileyObstetrics and Gynecology International1687-95891687-95972010-01-01201010.1155/2010/870865870865Specificity of Methylation Assays in Cancer Research: A Guideline for Designing Primers and ProbesZeinab Barekati0Ramin Radpour1Corina Kohler2Xiao Yan Zhong3Laboratory for Gynecological Oncology, Women's Hospital and Department of Biomedicine, University of Basel, Basel 4031, SwitzerlandLaboratory for Gynecological Oncology, Women's Hospital and Department of Biomedicine, University of Basel, Basel 4031, SwitzerlandLaboratory for Gynecological Oncology, Women's Hospital and Department of Biomedicine, University of Basel, Basel 4031, SwitzerlandLaboratory for Gynecological Oncology, Women's Hospital and Department of Biomedicine, University of Basel, Basel 4031, SwitzerlandDNA methylation is an epigenetic regulation mechanism of genomic function, and aberrant methylation pattern has been found to be a common event in many diseases and human cancers. A large number of cancer studies have been focused on identification of methylation changes as biomarkers (i.e., breast cancer). However, still clinical use of them is very limited because of lack of specificity and sensitivity for diagnostic test. This highlights the critical need for specific primer and probe design to avoid false-positive detection of methylation profiling. The guideline and online web tools that are introduced in this paper might help to perform a successful experiment and to develop specific diagnosis biomarkers by designing right primer pair and probe prior to experimental step.http://dx.doi.org/10.1155/2010/870865 |
| spellingShingle | Zeinab Barekati Ramin Radpour Corina Kohler Xiao Yan Zhong Specificity of Methylation Assays in Cancer Research: A Guideline for Designing Primers and Probes Obstetrics and Gynecology International |
| title | Specificity of Methylation Assays in Cancer Research: A Guideline for Designing Primers and Probes |
| title_full | Specificity of Methylation Assays in Cancer Research: A Guideline for Designing Primers and Probes |
| title_fullStr | Specificity of Methylation Assays in Cancer Research: A Guideline for Designing Primers and Probes |
| title_full_unstemmed | Specificity of Methylation Assays in Cancer Research: A Guideline for Designing Primers and Probes |
| title_short | Specificity of Methylation Assays in Cancer Research: A Guideline for Designing Primers and Probes |
| title_sort | specificity of methylation assays in cancer research a guideline for designing primers and probes |
| url | http://dx.doi.org/10.1155/2010/870865 |
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