Evaluation of LATS1 and LATS2 Promoter Methylation with the Risk of Pterygium Formation
Purpose. Pterygium is a serious eye problem in countries with high exposure to UV. However, despite numerous studies, the molecular etiology of pterygium is unclear. Recent studies have indicated that LATS1 and LATS2 genes are involved in DDR signaling pathways against continuous UV exposure. Our ai...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Ophthalmology |
| Online Access: | http://dx.doi.org/10.1155/2016/5431021 |
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| author | Maryam Najafi Dor Mohammad Kordi-Tamandani Mohammad Arish |
| author_facet | Maryam Najafi Dor Mohammad Kordi-Tamandani Mohammad Arish |
| author_sort | Maryam Najafi |
| collection | DOAJ |
| description | Purpose. Pterygium is a serious eye problem in countries with high exposure to UV. However, despite numerous studies, the molecular etiology of pterygium is unclear. Recent studies have indicated that LATS1 and LATS2 genes are involved in DDR signaling pathways against continuous UV exposure. Our aim was to evaluate the LATS1 and LATS2 promoter methylation with the risk of pterygium formation. Methods. We evaluated the promoter methylation status of LATS1 and LATS2 using methylation-specific PCR technique. Also, mRNA expression of LATS1 and LATS2 was assessed in 14 cases of pterygium and 14 normal specimens by real-time PCR. Results. Promoter methylation of LATS1 and LATS2 was detected significantly between pterygium tissues and normal tissues [LATS1; OR = 4.9; 95% CI: 1.54 to 15.48, P=0.003; LATS2; OR = 7.1; 95% CI: 1.53 to 33.19, P=0.004]. The gene expression analysis showed a statistically significant difference between pterygium tissues and healthy controls for both LATS1 and LATS2 (P<0.05). Conclusions. The data of this study is the first report regarding the effect of promoter methylation of the LATS1 and LATS2 in the pterygium. To confirm these data, doing further studies in various genetic populations with large sample sizes using advanced molecular techniques is proposed. |
| format | Article |
| id | doaj-art-ccfa48d1e2c647a4b0f512214f83d8cb |
| institution | OA Journals |
| issn | 2090-004X 2090-0058 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Ophthalmology |
| spelling | doaj-art-ccfa48d1e2c647a4b0f512214f83d8cb2025-08-20T02:19:54ZengWileyJournal of Ophthalmology2090-004X2090-00582016-01-01201610.1155/2016/54310215431021Evaluation of LATS1 and LATS2 Promoter Methylation with the Risk of Pterygium FormationMaryam Najafi0Dor Mohammad Kordi-Tamandani1Mohammad Arish2Departement of Biology, University of Sistan and Baluchestan, Zahedan, IranDepartement of Biology, University of Sistan and Baluchestan, Zahedan, IranDepartment of Ophthalmology, Al-Zahra Eye Hospital, Zahedan University of Medical Sciences, Zahedan 98167-43463, IranPurpose. Pterygium is a serious eye problem in countries with high exposure to UV. However, despite numerous studies, the molecular etiology of pterygium is unclear. Recent studies have indicated that LATS1 and LATS2 genes are involved in DDR signaling pathways against continuous UV exposure. Our aim was to evaluate the LATS1 and LATS2 promoter methylation with the risk of pterygium formation. Methods. We evaluated the promoter methylation status of LATS1 and LATS2 using methylation-specific PCR technique. Also, mRNA expression of LATS1 and LATS2 was assessed in 14 cases of pterygium and 14 normal specimens by real-time PCR. Results. Promoter methylation of LATS1 and LATS2 was detected significantly between pterygium tissues and normal tissues [LATS1; OR = 4.9; 95% CI: 1.54 to 15.48, P=0.003; LATS2; OR = 7.1; 95% CI: 1.53 to 33.19, P=0.004]. The gene expression analysis showed a statistically significant difference between pterygium tissues and healthy controls for both LATS1 and LATS2 (P<0.05). Conclusions. The data of this study is the first report regarding the effect of promoter methylation of the LATS1 and LATS2 in the pterygium. To confirm these data, doing further studies in various genetic populations with large sample sizes using advanced molecular techniques is proposed.http://dx.doi.org/10.1155/2016/5431021 |
| spellingShingle | Maryam Najafi Dor Mohammad Kordi-Tamandani Mohammad Arish Evaluation of LATS1 and LATS2 Promoter Methylation with the Risk of Pterygium Formation Journal of Ophthalmology |
| title | Evaluation of LATS1 and LATS2 Promoter Methylation with the Risk of Pterygium Formation |
| title_full | Evaluation of LATS1 and LATS2 Promoter Methylation with the Risk of Pterygium Formation |
| title_fullStr | Evaluation of LATS1 and LATS2 Promoter Methylation with the Risk of Pterygium Formation |
| title_full_unstemmed | Evaluation of LATS1 and LATS2 Promoter Methylation with the Risk of Pterygium Formation |
| title_short | Evaluation of LATS1 and LATS2 Promoter Methylation with the Risk of Pterygium Formation |
| title_sort | evaluation of lats1 and lats2 promoter methylation with the risk of pterygium formation |
| url | http://dx.doi.org/10.1155/2016/5431021 |
| work_keys_str_mv | AT maryamnajafi evaluationoflats1andlats2promotermethylationwiththeriskofpterygiumformation AT dormohammadkorditamandani evaluationoflats1andlats2promotermethylationwiththeriskofpterygiumformation AT mohammadarish evaluationoflats1andlats2promotermethylationwiththeriskofpterygiumformation |