An Open-label Randomized Crossover Study with Adaptive Design of the Pharmacokinetics and Bioequivalence of GP30121 and Ceraxon® Corrected for Endogenous Analyte Level

Introduction. Citicoline is an endogenous nucleoside consisting of cytidine and choline linked by a diphosphate bridge that is involved in the synthesis of membrane phospholipids. Drugs containing citicoline have neuroprotective and neurometabolic effects and are used for the treatment of a wide ran...

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Main Authors: A. N. Arevefa, A. R. Dorotenko, S. M. Noskov, I. E. Makarenko, R. V. Drai, T. N. Komarov, O. A. Archakova, N. S. Bagaeva, I. E. Shokhin
Format: Article
Language:Russian
Published: LLC Center of Pharmaceutical Analytics (LLC «CPHA») 2023-09-01
Series:Разработка и регистрация лекарственных средств
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Online Access:https://www.pharmjournal.ru/jour/article/view/1561
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author A. N. Arevefa
A. R. Dorotenko
S. M. Noskov
I. E. Makarenko
R. V. Drai
T. N. Komarov
O. A. Archakova
N. S. Bagaeva
I. E. Shokhin
author_facet A. N. Arevefa
A. R. Dorotenko
S. M. Noskov
I. E. Makarenko
R. V. Drai
T. N. Komarov
O. A. Archakova
N. S. Bagaeva
I. E. Shokhin
author_sort A. N. Arevefa
collection DOAJ
description Introduction. Citicoline is an endogenous nucleoside consisting of cytidine and choline linked by a diphosphate bridge that is involved in the synthesis of membrane phospholipids. Drugs containing citicoline have neuroprotective and neurometabolic effects and are used for the treatment of a wide range of neurological disorders. In its turn, bioequivalence study is a pathway to register a generic citicoline drug in Russian Federation.Aim. The aim of the study was to investigate the comparative pharmacokinetics (PK) and bioequivalence of two citicoline-containing drugs GP30121 and Ceraxon® in healthy male volunteers when taken on an empty stomach.Materials and methods. We evaluated the pharmacokinetics of citicoline-containing drugs corrected for endogenous analyte (uridine) level using an adaptive design. We determined uridine concentration by high-performance liquid chromatography with mass spectrometric detection. We used R Project software, version 3.6.3. for performing statistical analysis for the study.Results and discussion. GP30121 and Ceraxon® exhibited similar PK profiles. It was shown that the values of 94.12 % confidence interval (CI) at α = 0.0294 for the geometric mean ratios for the primary PK parameters of the main metabolite of the active ingredient of the investigated drugs were fully contained within the predefined equivalence limits of 80.00–125.00 %.Conclusion. The study demonstrates bioequivalence of GP30121 and Ceraxon® proving the approach with the correction for endogenous analyte could be considered in studies of other drugs.
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institution Kabale University
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2658-5049
language Russian
publishDate 2023-09-01
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series Разработка и регистрация лекарственных средств
spelling doaj-art-ccf7fc38cfed460b835470037a54f63e2025-08-20T03:35:00ZrusLLC Center of Pharmaceutical Analytics (LLC «CPHA»)Разработка и регистрация лекарственных средств2305-20662658-50492023-09-0112321822710.33380/2305-2066-2023-12-3-218-2271143An Open-label Randomized Crossover Study with Adaptive Design of the Pharmacokinetics and Bioequivalence of GP30121 and Ceraxon® Corrected for Endogenous Analyte LevelA. N. Arevefa0A. R. Dorotenko1S. M. Noskov2I. E. Makarenko3R. V. Drai4T. N. Komarov5O. A. Archakova6N. S. Bagaeva7I. E. Shokhin8CJSC "Pharm Holding"; Pavlov First Saint Petersburg State Medical University (Pavlov University)CJSC "Pharm Holding"; Pavlov First Saint Petersburg State Medical University (Pavlov University)Clinical Hospital No. 3CJSC "Pharm Holding"; Federal State Budgetary Educational Institution of Higher Education "A. I. Evdokimov Moscow State University of Medicine and Dentistry" of the Ministry of Healthcare of the Russian FederationCJSC "Pharm Holding"LLC "Center for Pharmaceutical Analytics"LLC "Center for Pharmaceutical Analytics"LLC "Center for Pharmaceutical Analytics"LLC "Center for Pharmaceutical Analytics"Introduction. Citicoline is an endogenous nucleoside consisting of cytidine and choline linked by a diphosphate bridge that is involved in the synthesis of membrane phospholipids. Drugs containing citicoline have neuroprotective and neurometabolic effects and are used for the treatment of a wide range of neurological disorders. In its turn, bioequivalence study is a pathway to register a generic citicoline drug in Russian Federation.Aim. The aim of the study was to investigate the comparative pharmacokinetics (PK) and bioequivalence of two citicoline-containing drugs GP30121 and Ceraxon® in healthy male volunteers when taken on an empty stomach.Materials and methods. We evaluated the pharmacokinetics of citicoline-containing drugs corrected for endogenous analyte (uridine) level using an adaptive design. We determined uridine concentration by high-performance liquid chromatography with mass spectrometric detection. We used R Project software, version 3.6.3. for performing statistical analysis for the study.Results and discussion. GP30121 and Ceraxon® exhibited similar PK profiles. It was shown that the values of 94.12 % confidence interval (CI) at α = 0.0294 for the geometric mean ratios for the primary PK parameters of the main metabolite of the active ingredient of the investigated drugs were fully contained within the predefined equivalence limits of 80.00–125.00 %.Conclusion. The study demonstrates bioequivalence of GP30121 and Ceraxon® proving the approach with the correction for endogenous analyte could be considered in studies of other drugs.https://www.pharmjournal.ru/jour/article/view/1561citicolineuridinebioavailabilityadaptive designsafety
spellingShingle A. N. Arevefa
A. R. Dorotenko
S. M. Noskov
I. E. Makarenko
R. V. Drai
T. N. Komarov
O. A. Archakova
N. S. Bagaeva
I. E. Shokhin
An Open-label Randomized Crossover Study with Adaptive Design of the Pharmacokinetics and Bioequivalence of GP30121 and Ceraxon® Corrected for Endogenous Analyte Level
Разработка и регистрация лекарственных средств
citicoline
uridine
bioavailability
adaptive design
safety
title An Open-label Randomized Crossover Study with Adaptive Design of the Pharmacokinetics and Bioequivalence of GP30121 and Ceraxon® Corrected for Endogenous Analyte Level
title_full An Open-label Randomized Crossover Study with Adaptive Design of the Pharmacokinetics and Bioequivalence of GP30121 and Ceraxon® Corrected for Endogenous Analyte Level
title_fullStr An Open-label Randomized Crossover Study with Adaptive Design of the Pharmacokinetics and Bioequivalence of GP30121 and Ceraxon® Corrected for Endogenous Analyte Level
title_full_unstemmed An Open-label Randomized Crossover Study with Adaptive Design of the Pharmacokinetics and Bioequivalence of GP30121 and Ceraxon® Corrected for Endogenous Analyte Level
title_short An Open-label Randomized Crossover Study with Adaptive Design of the Pharmacokinetics and Bioequivalence of GP30121 and Ceraxon® Corrected for Endogenous Analyte Level
title_sort open label randomized crossover study with adaptive design of the pharmacokinetics and bioequivalence of gp30121 and ceraxon r corrected for endogenous analyte level
topic citicoline
uridine
bioavailability
adaptive design
safety
url https://www.pharmjournal.ru/jour/article/view/1561
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