The activation of antiviral RNA interference not only exists in neural progenitor cells but also in somatic cells in mammals
The RNA interference (RNAi) pathway directs an important antiviral immunity mechanism in plants and invertebrates. Recently, we and others have demonstrated that the antiviral RNAi response is also conserved in mammals, at least to five distinct RNA viruses, including Zika virus (ZIKV). ZIKV may pre...
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Taylor & Francis Group
2020-01-01
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| Series: | Emerging Microbes and Infections |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2020.1787798 |
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| author | Yuqiang Zhang Zhe Li Zhi Ye Yan Xu Binbin Wang Congcong Wang Yunpeng Dai Jinfeng Lu Boxun Lu Wanju Zhang Yang Li |
| author_facet | Yuqiang Zhang Zhe Li Zhi Ye Yan Xu Binbin Wang Congcong Wang Yunpeng Dai Jinfeng Lu Boxun Lu Wanju Zhang Yang Li |
| author_sort | Yuqiang Zhang |
| collection | DOAJ |
| description | The RNA interference (RNAi) pathway directs an important antiviral immunity mechanism in plants and invertebrates. Recently, we and others have demonstrated that the antiviral RNAi response is also conserved in mammals, at least to five distinct RNA viruses, including Zika virus (ZIKV). ZIKV may preferentially infect neuronal progenitor cells (NPCs) in the developing foetal brain. Ex vivo ZIKV infection induces RNAi-mediated antiviral response in human NPCs, but not in the more differentiated NPCs or somatic cells. However, litter is known about the in vivo property or function of the virus-derived small-interfering RNAs (vsiRNAs) targeting ZIKV. Here we report a surprising observation: different from ex vivo observations, viral small RNAs (vsRNAs) targeting ZIKV were produced in vivo upon infection in both central neuron system (CNS) and muscle tissues. In addition, our findings demonstrate the production of canonical vsiRNAs in murine CNS upon antiviral RNAi activation by Sindbis virus (SINV), suggesting the possibility of antiviral immune strategy applied by mammals in the CNS. |
| format | Article |
| id | doaj-art-cceba0515f4a4e4b803053f70790c8df |
| institution | OA Journals |
| issn | 2222-1751 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj-art-cceba0515f4a4e4b803053f70790c8df2025-08-20T02:12:15ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512020-01-01911580158910.1080/22221751.2020.1787798The activation of antiviral RNA interference not only exists in neural progenitor cells but also in somatic cells in mammalsYuqiang Zhang0Zhe Li1Zhi Ye2Yan Xu3Binbin Wang4Congcong Wang5Yunpeng Dai6Jinfeng Lu7Boxun Lu8Wanju Zhang9Yang Li10State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, ChinaState Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, ChinaState Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, ChinaState Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, ChinaState Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, ChinaState Key Laboratory of Medical Neurobiology, School of Life Sciences, Fudan University, Shanghai, ChinaState Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, ChinaInstitute for Genomic Medicine, Columbia University Irving Medical Center, New York, NY, USAState Key Laboratory of Medical Neurobiology, School of Life Sciences, Fudan University, Shanghai, ChinaDepartment of Pathogen Diagnosis and Biosafety, Shanghai Public Health Clinical Center, Fudan University, Shanghai, ChinaState Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, ChinaThe RNA interference (RNAi) pathway directs an important antiviral immunity mechanism in plants and invertebrates. Recently, we and others have demonstrated that the antiviral RNAi response is also conserved in mammals, at least to five distinct RNA viruses, including Zika virus (ZIKV). ZIKV may preferentially infect neuronal progenitor cells (NPCs) in the developing foetal brain. Ex vivo ZIKV infection induces RNAi-mediated antiviral response in human NPCs, but not in the more differentiated NPCs or somatic cells. However, litter is known about the in vivo property or function of the virus-derived small-interfering RNAs (vsiRNAs) targeting ZIKV. Here we report a surprising observation: different from ex vivo observations, viral small RNAs (vsRNAs) targeting ZIKV were produced in vivo upon infection in both central neuron system (CNS) and muscle tissues. In addition, our findings demonstrate the production of canonical vsiRNAs in murine CNS upon antiviral RNAi activation by Sindbis virus (SINV), suggesting the possibility of antiviral immune strategy applied by mammals in the CNS.https://www.tandfonline.com/doi/10.1080/22221751.2020.1787798Zika virusantiviral immune responseantiviral RNA interferencepathogenesis of ZIKVimmune-compromised micevirus clearance |
| spellingShingle | Yuqiang Zhang Zhe Li Zhi Ye Yan Xu Binbin Wang Congcong Wang Yunpeng Dai Jinfeng Lu Boxun Lu Wanju Zhang Yang Li The activation of antiviral RNA interference not only exists in neural progenitor cells but also in somatic cells in mammals Emerging Microbes and Infections Zika virus antiviral immune response antiviral RNA interference pathogenesis of ZIKV immune-compromised mice virus clearance |
| title | The activation of antiviral RNA interference not only exists in neural progenitor cells but also in somatic cells in mammals |
| title_full | The activation of antiviral RNA interference not only exists in neural progenitor cells but also in somatic cells in mammals |
| title_fullStr | The activation of antiviral RNA interference not only exists in neural progenitor cells but also in somatic cells in mammals |
| title_full_unstemmed | The activation of antiviral RNA interference not only exists in neural progenitor cells but also in somatic cells in mammals |
| title_short | The activation of antiviral RNA interference not only exists in neural progenitor cells but also in somatic cells in mammals |
| title_sort | activation of antiviral rna interference not only exists in neural progenitor cells but also in somatic cells in mammals |
| topic | Zika virus antiviral immune response antiviral RNA interference pathogenesis of ZIKV immune-compromised mice virus clearance |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2020.1787798 |
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