An Analysis of Partial DNA Sequence of Meisa1 Gene on Sweet and Bitter Cassavas (Manihot esculenta Crantz.)
<p>Sweet and bitter taste on cassava tuber is affected by starch metabolisms. Meisa1 gene is a gene in cassava (Manihot esculenta Crantz.) encoding isoamylase1 enzyme involved in starch metabolisms. This study aimed to analyze partial DNA sequences of Meisa1 gene on sweet and bitter cassavas c...
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Universitas Negeri Semarang
2016-04-01
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| Series: | Biosaintifika: Journal of Biology & Biology Education |
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| Online Access: | https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/5365 |
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| author | Dewi Indriyani Roslim Fitriyatun Nisa Herman Herman |
| author_facet | Dewi Indriyani Roslim Fitriyatun Nisa Herman Herman |
| author_sort | Dewi Indriyani Roslim |
| collection | DOAJ |
| description | <p>Sweet and bitter taste on cassava tuber is affected by starch metabolisms. Meisa1 gene is a gene in cassava (Manihot esculenta Crantz.) encoding isoamylase1 enzyme involved in starch metabolisms. This study aimed to analyze partial DNA sequences of Meisa1 gene on sweet and bitter cassavas collected by Genetics Laboratory, Department Biology, Faculty of Mathematics and Natural Sciences, Riau University, Indonesia. Methods included total DNA extraction from fresh young leaves of cassava using CTAB buffer, polymerase chain reaction (PCR), electrophoresis, and sequencing. The obtained data were analyzed using MEGA software version 5. The results showed that there were nucleotide variations in the intron region, not in the exon region. The variations were caused by the transition substitution mutation (35.39%) and transversion substitution mutation (64.61%). The genetic distance range between seven cassava genotypes was approximately 0% to 11%. Partial DNA sequence variations of Meisa1 gene located in intron region were unable to cluster seven cassava genotypes separately into two groups based on tuber taste.</p><p><strong>How to Cite</strong></p><p>Roslim, D., Nisa, F., & Herman, H. (2016). An Analysis of Partial DNA Sequence of <em>Meisa1</em> Gene on Sweet and Bitter Cassavas (Manihot esculenta Crantz.). <em>Biosaintifika: Journal of Biology & Biology Education</em>, 8(1) 103-110.</p> |
| format | Article |
| id | doaj-art-cceb1e8e38e54c8ead5da412a9dc99cd |
| institution | OA Journals |
| issn | 2085-191X 2338-7610 |
| language | English |
| publishDate | 2016-04-01 |
| publisher | Universitas Negeri Semarang |
| record_format | Article |
| series | Biosaintifika: Journal of Biology & Biology Education |
| spelling | doaj-art-cceb1e8e38e54c8ead5da412a9dc99cd2025-08-20T02:02:45ZengUniversitas Negeri SemarangBiosaintifika: Journal of Biology & Biology Education2085-191X2338-76102016-04-018110511210.15294/biosaintifika.v8i1.53654266An Analysis of Partial DNA Sequence of Meisa1 Gene on Sweet and Bitter Cassavas (Manihot esculenta Crantz.)Dewi Indriyani Roslim0Fitriyatun Nisa1Herman Herman2Department of Biology, Faculty of Mathematics and Natural Science, Universitas Riau, Indonesia, Kampus Bina Widya, Jl. HR Soebrantas, Panam, Pekanbaru 28293, RiauDepartment of Biology, Faculty of Mathematics and Natural Science, Universitas Riau, Indonesia, Kampus Bina Widya, Jl. HR Soebrantas, Panam, Pekanbaru 28293, RiauDepartment of Biology, Faculty of Mathematics and Natural Science, Universitas Riau, Indonesia, Kampus Bina Widya, Jl. HR Soebrantas, Panam, Pekanbaru 28293, Riau<p>Sweet and bitter taste on cassava tuber is affected by starch metabolisms. Meisa1 gene is a gene in cassava (Manihot esculenta Crantz.) encoding isoamylase1 enzyme involved in starch metabolisms. This study aimed to analyze partial DNA sequences of Meisa1 gene on sweet and bitter cassavas collected by Genetics Laboratory, Department Biology, Faculty of Mathematics and Natural Sciences, Riau University, Indonesia. Methods included total DNA extraction from fresh young leaves of cassava using CTAB buffer, polymerase chain reaction (PCR), electrophoresis, and sequencing. The obtained data were analyzed using MEGA software version 5. The results showed that there were nucleotide variations in the intron region, not in the exon region. The variations were caused by the transition substitution mutation (35.39%) and transversion substitution mutation (64.61%). The genetic distance range between seven cassava genotypes was approximately 0% to 11%. Partial DNA sequence variations of Meisa1 gene located in intron region were unable to cluster seven cassava genotypes separately into two groups based on tuber taste.</p><p><strong>How to Cite</strong></p><p>Roslim, D., Nisa, F., & Herman, H. (2016). An Analysis of Partial DNA Sequence of <em>Meisa1</em> Gene on Sweet and Bitter Cassavas (Manihot esculenta Crantz.). <em>Biosaintifika: Journal of Biology & Biology Education</em>, 8(1) 103-110.</p>https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/5365DNA sequenceManihot esculenta CrantzMeisa1 genemutationPCR |
| spellingShingle | Dewi Indriyani Roslim Fitriyatun Nisa Herman Herman An Analysis of Partial DNA Sequence of Meisa1 Gene on Sweet and Bitter Cassavas (Manihot esculenta Crantz.) Biosaintifika: Journal of Biology & Biology Education DNA sequence Manihot esculenta Crantz Meisa1 gene mutation PCR |
| title | An Analysis of Partial DNA Sequence of Meisa1 Gene on Sweet and Bitter Cassavas (Manihot esculenta Crantz.) |
| title_full | An Analysis of Partial DNA Sequence of Meisa1 Gene on Sweet and Bitter Cassavas (Manihot esculenta Crantz.) |
| title_fullStr | An Analysis of Partial DNA Sequence of Meisa1 Gene on Sweet and Bitter Cassavas (Manihot esculenta Crantz.) |
| title_full_unstemmed | An Analysis of Partial DNA Sequence of Meisa1 Gene on Sweet and Bitter Cassavas (Manihot esculenta Crantz.) |
| title_short | An Analysis of Partial DNA Sequence of Meisa1 Gene on Sweet and Bitter Cassavas (Manihot esculenta Crantz.) |
| title_sort | analysis of partial dna sequence of meisa1 gene on sweet and bitter cassavas manihot esculenta crantz |
| topic | DNA sequence Manihot esculenta Crantz Meisa1 gene mutation PCR |
| url | https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/5365 |
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