Expression of α<sub>v</sub> Integrin in Feline Injection-Site Sarcoma (FISS): Preliminary Investigations
Feline injection-site sarcomas (FISSs) are malignant skin tumors of mesenchymal origin arising at local post-vaccination (or injection) sites. In recent years, a fluorescence imaging technique based on probes targeting α<sub>v</sub>β<sub>3</sub> integrin has been effectively...
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2024-12-01
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| author | Andrea Cappelleri Eleonora Brambilla Lavinia E. Chiti Alessia Trapletti Gaia B. M. Bianchi Mauro Di Giancamillo Valeria Grieco Chiara Giudice |
| author_facet | Andrea Cappelleri Eleonora Brambilla Lavinia E. Chiti Alessia Trapletti Gaia B. M. Bianchi Mauro Di Giancamillo Valeria Grieco Chiara Giudice |
| author_sort | Andrea Cappelleri |
| collection | DOAJ |
| description | Feline injection-site sarcomas (FISSs) are malignant skin tumors of mesenchymal origin arising at local post-vaccination (or injection) sites. In recent years, a fluorescence imaging technique based on probes targeting α<sub>v</sub>β<sub>3</sub> integrin has been effectively applied for the surgical complete resection of the tumor. In our study, we investigated the utility of a commercially available anti-α<sub>v</sub> integrin polyclonal antibody for the histopathological evaluation of FISS’s surgical excision margins. We collected 10 formalin-fixed paraffin-embedded (FFPE) feline excisional biopsies with a histopathological diagnosis of FISS (7 fibrosarcomas and 3 pleomorphic sarcomas) and wide margin tissue, along with one subcutaneous injection-site granuloma and 6 osteosarcomas. Samples were processed for histology, and slides were stained for IHC with the anti-α<sub>v</sub> integrin antibody. Immunostained slides were evaluated for the cellular localization and intensity of the staining in different neoplastic and non-neoplastic cell populations. Neoplastic and non-neoplastic spindle cells had cytoplasmic positivity in all fibrosarcomas. Inflammatory cells, including macrophages of the injection-site granuloma, were negative. Multinucleated giant cells in the pleomorphic sarcomas had an intense membranous positivity. Although the anti-α<sub>v</sub> integrin antibody was ineffective for the histopathological evaluation of surgical excision margins, the membranous localization of α<sub>v</sub> integrin in multinucleated giant cells of pleomorphic sarcomas suggests that it plays a role in the oncogenesis of this FISS variant. |
| format | Article |
| id | doaj-art-cceaf7b0a43e44e0bce96fc215f68d75 |
| institution | DOAJ |
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| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Animals |
| spelling | doaj-art-cceaf7b0a43e44e0bce96fc215f68d752025-08-20T02:50:52ZengMDPI AGAnimals2076-26152024-12-011424358810.3390/ani14243588Expression of α<sub>v</sub> Integrin in Feline Injection-Site Sarcoma (FISS): Preliminary InvestigationsAndrea Cappelleri0Eleonora Brambilla1Lavinia E. Chiti2Alessia Trapletti3Gaia B. M. Bianchi4Mauro Di Giancamillo5Valeria Grieco6Chiara Giudice7Department of Veterinary Medicine and Animal Sciences, University of Milan, 26900 Lodi, ItalyDepartment of Veterinary Medicine and Animal Sciences, University of Milan, 26900 Lodi, ItalyClinic for Small Animal Surgery, Department for Small Animals, Vetsuisse Faculty, University of Zurich, 8057 Zurich, SwitzerlandDepartment of Veterinary Medicine and Animal Sciences, University of Milan, 26900 Lodi, ItalyDepartment of Veterinary Medicine and Animal Sciences, University of Milan, 26900 Lodi, ItalyDepartment of Veterinary Medicine and Animal Sciences, University of Milan, 26900 Lodi, ItalyDepartment of Veterinary Medicine and Animal Sciences, University of Milan, 26900 Lodi, ItalyDepartment of Veterinary Medicine and Animal Sciences, University of Milan, 26900 Lodi, ItalyFeline injection-site sarcomas (FISSs) are malignant skin tumors of mesenchymal origin arising at local post-vaccination (or injection) sites. In recent years, a fluorescence imaging technique based on probes targeting α<sub>v</sub>β<sub>3</sub> integrin has been effectively applied for the surgical complete resection of the tumor. In our study, we investigated the utility of a commercially available anti-α<sub>v</sub> integrin polyclonal antibody for the histopathological evaluation of FISS’s surgical excision margins. We collected 10 formalin-fixed paraffin-embedded (FFPE) feline excisional biopsies with a histopathological diagnosis of FISS (7 fibrosarcomas and 3 pleomorphic sarcomas) and wide margin tissue, along with one subcutaneous injection-site granuloma and 6 osteosarcomas. Samples were processed for histology, and slides were stained for IHC with the anti-α<sub>v</sub> integrin antibody. Immunostained slides were evaluated for the cellular localization and intensity of the staining in different neoplastic and non-neoplastic cell populations. Neoplastic and non-neoplastic spindle cells had cytoplasmic positivity in all fibrosarcomas. Inflammatory cells, including macrophages of the injection-site granuloma, were negative. Multinucleated giant cells in the pleomorphic sarcomas had an intense membranous positivity. Although the anti-α<sub>v</sub> integrin antibody was ineffective for the histopathological evaluation of surgical excision margins, the membranous localization of α<sub>v</sub> integrin in multinucleated giant cells of pleomorphic sarcomas suggests that it plays a role in the oncogenesis of this FISS variant.https://www.mdpi.com/2076-2615/14/24/3588FISSintegrinimmunohistochemistryfibrosarcomapleomorphic sarcomainjection-site granuloma |
| spellingShingle | Andrea Cappelleri Eleonora Brambilla Lavinia E. Chiti Alessia Trapletti Gaia B. M. Bianchi Mauro Di Giancamillo Valeria Grieco Chiara Giudice Expression of α<sub>v</sub> Integrin in Feline Injection-Site Sarcoma (FISS): Preliminary Investigations Animals FISS integrin immunohistochemistry fibrosarcoma pleomorphic sarcoma injection-site granuloma |
| title | Expression of α<sub>v</sub> Integrin in Feline Injection-Site Sarcoma (FISS): Preliminary Investigations |
| title_full | Expression of α<sub>v</sub> Integrin in Feline Injection-Site Sarcoma (FISS): Preliminary Investigations |
| title_fullStr | Expression of α<sub>v</sub> Integrin in Feline Injection-Site Sarcoma (FISS): Preliminary Investigations |
| title_full_unstemmed | Expression of α<sub>v</sub> Integrin in Feline Injection-Site Sarcoma (FISS): Preliminary Investigations |
| title_short | Expression of α<sub>v</sub> Integrin in Feline Injection-Site Sarcoma (FISS): Preliminary Investigations |
| title_sort | expression of α sub v sub integrin in feline injection site sarcoma fiss preliminary investigations |
| topic | FISS integrin immunohistochemistry fibrosarcoma pleomorphic sarcoma injection-site granuloma |
| url | https://www.mdpi.com/2076-2615/14/24/3588 |
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