Sequencing Analysis of Invasive Carbapenem-Resistant <i>Klebsiella pneumoniae</i> Isolates Secondary to Gastrointestinal Colonization
<i>Klebsiella pneumoniae</i> represent a common invasive infection etiological agent, whose potential carbapenem-resistance and hypermucoviscosity complicate the patient’s management. Infection development often derives from gastrointestinal colonization; thus, it is fundamental to monit...
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2025-01-01
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| author | Gaetano Maugeri Maddalena Calvo Dafne Bongiorno Dalida Bivona Giuseppe Migliorisi Grete Francesca Privitera Guido Scalia Stefania Stefani |
| author_facet | Gaetano Maugeri Maddalena Calvo Dafne Bongiorno Dalida Bivona Giuseppe Migliorisi Grete Francesca Privitera Guido Scalia Stefania Stefani |
| author_sort | Gaetano Maugeri |
| collection | DOAJ |
| description | <i>Klebsiella pneumoniae</i> represent a common invasive infection etiological agent, whose potential carbapenem-resistance and hypermucoviscosity complicate the patient’s management. Infection development often derives from gastrointestinal colonization; thus, it is fundamental to monitor asymptomatic <i>K. pneumoniae</i> colonization through surveillance protocols, especially for intensive care and immunocompromised patients. We described a six-month routine screening protocol from the Policlinico of Catania (Italy), while blood samples were collected from the same patients only in cases of a systemic infection suspicion. All the patients who had dissemination episodes were furtherly investigated through next-generation sequencing, analyzing both colonizing and disseminating strains. This study documents emerging invasive sequence types such as ST101, ST307, and ST395, mainly revealing <i>blaNDM</i> or <i>blaKPC</i> genes, along with siderophores and hyperproduction capsule markers as virulence factors. Most of the detected factors are presumably related to a specific plasmid content, which are extremely varied and rich. In conclusion, active surveillance through sequencing is essential to enhance awareness of local epidemiology within high-risk multi-drug resistance areas. A random sequencing analysis on the most warning microorganisms could enhance sequence typing (ST) awareness within specific settings, allowing for better prevention control strategies on their eventual persistence or diffusion. |
| format | Article |
| id | doaj-art-ccea67261b15483d9f47a2f0c7dd2e42 |
| institution | Kabale University |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-01-01 |
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| spelling | doaj-art-ccea67261b15483d9f47a2f0c7dd2e422025-01-24T13:42:36ZengMDPI AGMicroorganisms2076-26072025-01-011318910.3390/microorganisms13010089Sequencing Analysis of Invasive Carbapenem-Resistant <i>Klebsiella pneumoniae</i> Isolates Secondary to Gastrointestinal ColonizationGaetano Maugeri0Maddalena Calvo1Dafne Bongiorno2Dalida Bivona3Giuseppe Migliorisi4Grete Francesca Privitera5Guido Scalia6Stefania Stefani7Microbiology Section, Department of Biomedical and Biotechnological Science, University of Catania, Via Santa Sofia 97, 95123 Catania, ItalyU.O.C. Laboratory Analysis Unit, A.O.U. “Policlinico-San Marco”, Via Santa Sofia 78, 95123 Catania, ItalyMicrobiology Section, Department of Biomedical and Biotechnological Science, University of Catania, Via Santa Sofia 97, 95123 Catania, ItalyMicrobiology Section, Department of Biomedical and Biotechnological Science, University of Catania, Via Santa Sofia 97, 95123 Catania, ItalyU.O.C. Laboratory Analysis Unit, A.O. “G.F. Ingrassia”, Corso Calatafimi 1002, 90131 Palermo, ItalyDepartment of Clinical and Experimental Medicine, Bioinformatics Unit, University of Catania, Via Santa Sofia 97, 95123 Catania, ItalyMicrobiology Section, Department of Biomedical and Biotechnological Science, University of Catania, Via Santa Sofia 97, 95123 Catania, ItalyMicrobiology Section, Department of Biomedical and Biotechnological Science, University of Catania, Via Santa Sofia 97, 95123 Catania, Italy<i>Klebsiella pneumoniae</i> represent a common invasive infection etiological agent, whose potential carbapenem-resistance and hypermucoviscosity complicate the patient’s management. Infection development often derives from gastrointestinal colonization; thus, it is fundamental to monitor asymptomatic <i>K. pneumoniae</i> colonization through surveillance protocols, especially for intensive care and immunocompromised patients. We described a six-month routine screening protocol from the Policlinico of Catania (Italy), while blood samples were collected from the same patients only in cases of a systemic infection suspicion. All the patients who had dissemination episodes were furtherly investigated through next-generation sequencing, analyzing both colonizing and disseminating strains. This study documents emerging invasive sequence types such as ST101, ST307, and ST395, mainly revealing <i>blaNDM</i> or <i>blaKPC</i> genes, along with siderophores and hyperproduction capsule markers as virulence factors. Most of the detected factors are presumably related to a specific plasmid content, which are extremely varied and rich. In conclusion, active surveillance through sequencing is essential to enhance awareness of local epidemiology within high-risk multi-drug resistance areas. A random sequencing analysis on the most warning microorganisms could enhance sequence typing (ST) awareness within specific settings, allowing for better prevention control strategies on their eventual persistence or diffusion.https://www.mdpi.com/2076-2607/13/1/89<i>Klebsiella pneumoniae</i>gastrointestinal colonizationsystemic infectionresistomevirulomeplasmids |
| spellingShingle | Gaetano Maugeri Maddalena Calvo Dafne Bongiorno Dalida Bivona Giuseppe Migliorisi Grete Francesca Privitera Guido Scalia Stefania Stefani Sequencing Analysis of Invasive Carbapenem-Resistant <i>Klebsiella pneumoniae</i> Isolates Secondary to Gastrointestinal Colonization Microorganisms <i>Klebsiella pneumoniae</i> gastrointestinal colonization systemic infection resistome virulome plasmids |
| title | Sequencing Analysis of Invasive Carbapenem-Resistant <i>Klebsiella pneumoniae</i> Isolates Secondary to Gastrointestinal Colonization |
| title_full | Sequencing Analysis of Invasive Carbapenem-Resistant <i>Klebsiella pneumoniae</i> Isolates Secondary to Gastrointestinal Colonization |
| title_fullStr | Sequencing Analysis of Invasive Carbapenem-Resistant <i>Klebsiella pneumoniae</i> Isolates Secondary to Gastrointestinal Colonization |
| title_full_unstemmed | Sequencing Analysis of Invasive Carbapenem-Resistant <i>Klebsiella pneumoniae</i> Isolates Secondary to Gastrointestinal Colonization |
| title_short | Sequencing Analysis of Invasive Carbapenem-Resistant <i>Klebsiella pneumoniae</i> Isolates Secondary to Gastrointestinal Colonization |
| title_sort | sequencing analysis of invasive carbapenem resistant i klebsiella pneumoniae i isolates secondary to gastrointestinal colonization |
| topic | <i>Klebsiella pneumoniae</i> gastrointestinal colonization systemic infection resistome virulome plasmids |
| url | https://www.mdpi.com/2076-2607/13/1/89 |
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