Rapid Genotyping of <i>FecB</i> Mutation in Sheep Using CRISPR-Cas12a Integrated with DNA Nanotree Biosensing Platform

Rapid Genotyping of <i>FecB</i> Mutation in Sheep Using CRISPR-Cas12a Integrated with DNA Nanotree Biosensing Platform

The A-to-G mutation (<i>FecB</i>) in the <i>BMPR1B</i> gene is strongly linked to fertility in sheep, significantly increasing ovulation rates and litter sizes compared to wild-type populations. The rapid and reliable screening of the <i>FecB</i> gene is therefore...

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Bibliographic Details
Main Authors: Dongxia Pan, Mukaddas Mijit, Hui Wang, Chaoqun Sun, Bantan Pingcuo, Zhixue Yu, Benhai Xiong, Xiangfang Tang
Format: Article
Language:English
Published: MDPI AG 2025-02-01
Series:Biomolecules
Subjects:
CRISPR-Cas12a
amplification retarded mutation system
<i>FecB</i>
Fe<sub>3</sub>O<sub>4</sub>@Au-Linker-Cy5-Nanotree
rapid detection
Online Access:https://www.mdpi.com/2218-273X/15/3/315
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