Regulation of collagenolytic activity using platelet preparations <i>in vitro</i>
Purpose: to study different platelet preparations' impact on collagenase activity in vitro. Materials and methods. Human amnion transplants and collagen covers, based on human type 1 collagen, were used as collagen matrices. The drug Fermencol was used as a source of collagen-lytic peptidases....
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| Format: | Article |
| Language: | Russian |
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Real Time Ltd
2025-03-01
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| Series: | Российский офтальмологический журнал |
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| Online Access: | https://roj.igb.ru/jour/article/view/1714 |
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| author | E. V. Chentsova N. V. Borovkova K. V. Sirotkina M. S. Makarov M. V. Storozheva I. N. Ponomarev |
| author_facet | E. V. Chentsova N. V. Borovkova K. V. Sirotkina M. S. Makarov M. V. Storozheva I. N. Ponomarev |
| author_sort | E. V. Chentsova |
| collection | DOAJ |
| description | Purpose: to study different platelet preparations' impact on collagenase activity in vitro. Materials and methods. Human amnion transplants and collagen covers, based on human type 1 collagen, were used as collagen matrices. The drug Fermencol was used as a source of collagen-lytic peptidases. We used platelet-rich plasma (PRP); concentrated suspension of platelets washed from plasma (WP); platelet-poor plasma (PPP); lysates of PRP, PPP and WP. To assess the collagen-lytic activity we studied the level of collagen autofluorescence in tissue grafts, collagen concentration in solution, proliferation intensity of mesenchymal multipotent stromal cells (MMSC) on the surface of the grafts. Results. Preparations based on PRP and WP stimulated significant inhibition of collagen-lytic activity, which was absent in PPP. The inhibition of collagen-lytic effect in PRP and WP depended on the initial content of platelets with granules. Platelet preparations significantly enhanced the adhesion and growth of MMSC on the surface of the amnion and collagen cover. Conclusion. The use of platelet lysates combined high inhibition of collagen-lytic peptidases and growth-stimulating effect, which persists even with dilution of PRP lysate in ratio 1:200. For effective inhibition of collagen-lytic peptidases is recommended to use PRP with initial concentration of platelets with granules at least 350 thousand/ml. |
| format | Article |
| id | doaj-art-ccdcdc4c50cc4d28afc900f5e61d7dfa |
| institution | DOAJ |
| issn | 2072-0076 2587-5760 |
| language | Russian |
| publishDate | 2025-03-01 |
| publisher | Real Time Ltd |
| record_format | Article |
| series | Российский офтальмологический журнал |
| spelling | doaj-art-ccdcdc4c50cc4d28afc900f5e61d7dfa2025-08-20T02:54:11ZrusReal Time LtdРоссийский офтальмологический журнал2072-00762587-57602025-03-01181929910.21516/2072-0076-2025-18-1-92-99762Regulation of collagenolytic activity using platelet preparations <i>in vitro</i>E. V. Chentsova0N. V. Borovkova1K. V. Sirotkina2M. S. Makarov3M. V. Storozheva4I. N. Ponomarev5Helmholtz National Medical Research Center of Eye DiseasesN.V. Sklifosovsky Research Institute for Emergency Medicine; Pirogov Russian National Research Medical UniversityHelmholtz National Medical Research Center of Eye DiseasesN.V. Sklifosovsky Research Institute for Emergency MedicineN.V. Sklifosovsky Research Institute for Emergency MedicineN.V. Sklifosovsky Research Institute for Emergency MedicinePurpose: to study different platelet preparations' impact on collagenase activity in vitro. Materials and methods. Human amnion transplants and collagen covers, based on human type 1 collagen, were used as collagen matrices. The drug Fermencol was used as a source of collagen-lytic peptidases. We used platelet-rich plasma (PRP); concentrated suspension of platelets washed from plasma (WP); platelet-poor plasma (PPP); lysates of PRP, PPP and WP. To assess the collagen-lytic activity we studied the level of collagen autofluorescence in tissue grafts, collagen concentration in solution, proliferation intensity of mesenchymal multipotent stromal cells (MMSC) on the surface of the grafts. Results. Preparations based on PRP and WP stimulated significant inhibition of collagen-lytic activity, which was absent in PPP. The inhibition of collagen-lytic effect in PRP and WP depended on the initial content of platelets with granules. Platelet preparations significantly enhanced the adhesion and growth of MMSC on the surface of the amnion and collagen cover. Conclusion. The use of platelet lysates combined high inhibition of collagen-lytic peptidases and growth-stimulating effect, which persists even with dilution of PRP lysate in ratio 1:200. For effective inhibition of collagen-lytic peptidases is recommended to use PRP with initial concentration of platelets with granules at least 350 thousand/ml.https://roj.igb.ru/jour/article/view/1714amnioncollagenplateletsanti-collagenase activityautofluorescenceproliferation |
| spellingShingle | E. V. Chentsova N. V. Borovkova K. V. Sirotkina M. S. Makarov M. V. Storozheva I. N. Ponomarev Regulation of collagenolytic activity using platelet preparations <i>in vitro</i> Российский офтальмологический журнал amnion collagen platelets anti-collagenase activity autofluorescence proliferation |
| title | Regulation of collagenolytic activity using platelet preparations <i>in vitro</i> |
| title_full | Regulation of collagenolytic activity using platelet preparations <i>in vitro</i> |
| title_fullStr | Regulation of collagenolytic activity using platelet preparations <i>in vitro</i> |
| title_full_unstemmed | Regulation of collagenolytic activity using platelet preparations <i>in vitro</i> |
| title_short | Regulation of collagenolytic activity using platelet preparations <i>in vitro</i> |
| title_sort | regulation of collagenolytic activity using platelet preparations i in vitro i |
| topic | amnion collagen platelets anti-collagenase activity autofluorescence proliferation |
| url | https://roj.igb.ru/jour/article/view/1714 |
| work_keys_str_mv | AT evchentsova regulationofcollagenolyticactivityusingplateletpreparationsiinvitroi AT nvborovkova regulationofcollagenolyticactivityusingplateletpreparationsiinvitroi AT kvsirotkina regulationofcollagenolyticactivityusingplateletpreparationsiinvitroi AT msmakarov regulationofcollagenolyticactivityusingplateletpreparationsiinvitroi AT mvstorozheva regulationofcollagenolyticactivityusingplateletpreparationsiinvitroi AT inponomarev regulationofcollagenolyticactivityusingplateletpreparationsiinvitroi |