Analysis in heredity and variation of major structural protein genes of PRRSV in swine from Hebei Province
Three pairs of primers were designed according to the published nucleotide sequences of porcine reproductive and respiratory syndrome virus (PRRSV) in GenBank, and to amplify complete ORF5-7 gene of PRRSV HB-3 (cz), HB-4 (hs) strains by RT-PCR. The PCR products were cloned into pGM-T vector. The rec...
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Zhejiang University Press
2009-05-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2009.03.006 |
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| author | CHEN Sai-juan LIU Tao SUN Ji-guo GU Zi-lin LOU Hua |
| author_facet | CHEN Sai-juan LIU Tao SUN Ji-guo GU Zi-lin LOU Hua |
| author_sort | CHEN Sai-juan |
| collection | DOAJ |
| description | Three pairs of primers were designed according to the published nucleotide sequences of porcine reproductive and respiratory syndrome virus (PRRSV) in GenBank, and to amplify complete ORF5-7 gene of PRRSV HB-3 (cz), HB-4 (hs) strains by RT-PCR. The PCR products were cloned into pGM-T vector. The recombinant plasmid were sequenced and compared with sequences of other PRRSV strains in GenBank. The results show that the 9 strains isolated from Hebei Province belonged to American genotype; the ORF5-7 gene of them have 98.8%-99.5% homologies in nucleotide level with the strains isolated from China in recent years, and the antigenic epitope regions of major structural protein were homologous; whereas it was only 91.9%-92.6% homologies with the strains isolated from China in last years. Phylogenetic tree analysis showed that the domestic strains could be divided into two subgroups according to the genetic distance, and the Hebei strains belonged to one subgroup. |
| format | Article |
| id | doaj-art-ccd7fcf388354981a7e6a21e34c3df5a |
| institution | Kabale University |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2009-05-01 |
| publisher | Zhejiang University Press |
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| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-ccd7fcf388354981a7e6a21e34c3df5a2025-08-20T03:33:22ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552009-05-013526627110.3785/j.issn.1008-9209.2009.03.00610089209Analysis in heredity and variation of major structural protein genes of PRRSV in swine from Hebei ProvinceCHEN Sai-juanLIU TaoSUN Ji-guoGU Zi-linLOU HuaThree pairs of primers were designed according to the published nucleotide sequences of porcine reproductive and respiratory syndrome virus (PRRSV) in GenBank, and to amplify complete ORF5-7 gene of PRRSV HB-3 (cz), HB-4 (hs) strains by RT-PCR. The PCR products were cloned into pGM-T vector. The recombinant plasmid were sequenced and compared with sequences of other PRRSV strains in GenBank. The results show that the 9 strains isolated from Hebei Province belonged to American genotype; the ORF5-7 gene of them have 98.8%-99.5% homologies in nucleotide level with the strains isolated from China in recent years, and the antigenic epitope regions of major structural protein were homologous; whereas it was only 91.9%-92.6% homologies with the strains isolated from China in last years. Phylogenetic tree analysis showed that the domestic strains could be divided into two subgroups according to the genetic distance, and the Hebei strains belonged to one subgroup.https://www.academax.com/doi/10.3785/j.issn.1008-9209.2009.03.006porcine reproductive and respiratory syndrome virusHebei strains<italic>ORF5-7</italic> geneheredity and variation analysis |
| spellingShingle | CHEN Sai-juan LIU Tao SUN Ji-guo GU Zi-lin LOU Hua Analysis in heredity and variation of major structural protein genes of PRRSV in swine from Hebei Province 浙江大学学报. 农业与生命科学版 porcine reproductive and respiratory syndrome virus Hebei strains <italic>ORF5-7</italic> gene heredity and variation analysis |
| title | Analysis in heredity and variation of major structural protein genes of PRRSV in swine from Hebei Province |
| title_full | Analysis in heredity and variation of major structural protein genes of PRRSV in swine from Hebei Province |
| title_fullStr | Analysis in heredity and variation of major structural protein genes of PRRSV in swine from Hebei Province |
| title_full_unstemmed | Analysis in heredity and variation of major structural protein genes of PRRSV in swine from Hebei Province |
| title_short | Analysis in heredity and variation of major structural protein genes of PRRSV in swine from Hebei Province |
| title_sort | analysis in heredity and variation of major structural protein genes of prrsv in swine from hebei province |
| topic | porcine reproductive and respiratory syndrome virus Hebei strains <italic>ORF5-7</italic> gene heredity and variation analysis |
| url | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2009.03.006 |
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