Poly(ARTEMA), a novel artesunate-based polymer induces ferroptosis in breast cancer cells
Ferroptosis, a form of non-apoptotic cell death, is emerging as a promising strategy for cancer therapy. Artesunate (ART), an extract obtained from the traditional Chinese medicine Qinghaosu, has been shown to exhibit anti-cancer activity by inducing ferroptosis in cancer cells. While previous resea...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Science and Technology of Advanced Materials |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/14686996.2025.2482514 |
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| author | Natsumi Ito Ahmed Nabil Koichiro Uto Mitsuhiro Ebara |
| author_facet | Natsumi Ito Ahmed Nabil Koichiro Uto Mitsuhiro Ebara |
| author_sort | Natsumi Ito |
| collection | DOAJ |
| description | Ferroptosis, a form of non-apoptotic cell death, is emerging as a promising strategy for cancer therapy. Artesunate (ART), an extract obtained from the traditional Chinese medicine Qinghaosu, has been shown to exhibit anti-cancer activity by inducing ferroptosis in cancer cells. While previous research has focused on incorporating ART monomer into drug delivery systems for enhanced cancer targeting, this study presents 2-methacryloyloxyethyl ART polymer (poly(ARTEMA)), a novel polymer synthesized from ART for the first time. Our goal was evaluation of poly(ARTEMA) anticancer potential on breast cancer cells. First, we synthesized ARTEMA using esterification followed by its polymerization using the reversible addition-fragmentation chain transfer (RAFT) polymerization method. We evaluated its mechanism of action, focusing on two key pathways: temperature-triggered singlet oxygen generation and ferrous ions (Fe2+) release, both of which contribute to ferroptosis. Our results demonstrate that poly(ARTEMA) selectively generates singlet oxygen and Fe2+ due to the endoperoxide crosslinks, leading to cell death in breast cancer cells. We also investigated the anti-cancer potential of poly(ARTEMA) on breast cancer cells with and without a ferroptosis inhibitor. The IC50 values were 125 µM for the MCF-7 cancer cell line and 300 µM for the normal MCF-10 cell line, indicating enhanced toxicity toward cancer cell lines. These findings suggested that poly(ARTEMA) induces ferroptosis in cancer cells and may serve as a promising candidate for cancer therapy with minimal cytotoxicity. To the best of our knowledge, this report may be the first that successfully synthesized poly(ARTEMA) using ART, with its anticancer potential evaluation. |
| format | Article |
| id | doaj-art-ccd45d46d90a484d965cd04d14b3c437 |
| institution | OA Journals |
| issn | 1468-6996 1878-5514 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Science and Technology of Advanced Materials |
| spelling | doaj-art-ccd45d46d90a484d965cd04d14b3c4372025-08-20T02:26:33ZengTaylor & Francis GroupScience and Technology of Advanced Materials1468-69961878-55142025-12-0126110.1080/14686996.2025.2482514Poly(ARTEMA), a novel artesunate-based polymer induces ferroptosis in breast cancer cellsNatsumi Ito0Ahmed Nabil1Koichiro Uto2Mitsuhiro Ebara3Research Center for Macromolecules and Biomaterials, National Institute for Materials Science (NIMS), Tsukuba, JapanResearch Center for Macromolecules and Biomaterials, National Institute for Materials Science (NIMS), Tsukuba, JapanResearch Center for Macromolecules and Biomaterials, National Institute for Materials Science (NIMS), Tsukuba, JapanResearch Center for Macromolecules and Biomaterials, National Institute for Materials Science (NIMS), Tsukuba, JapanFerroptosis, a form of non-apoptotic cell death, is emerging as a promising strategy for cancer therapy. Artesunate (ART), an extract obtained from the traditional Chinese medicine Qinghaosu, has been shown to exhibit anti-cancer activity by inducing ferroptosis in cancer cells. While previous research has focused on incorporating ART monomer into drug delivery systems for enhanced cancer targeting, this study presents 2-methacryloyloxyethyl ART polymer (poly(ARTEMA)), a novel polymer synthesized from ART for the first time. Our goal was evaluation of poly(ARTEMA) anticancer potential on breast cancer cells. First, we synthesized ARTEMA using esterification followed by its polymerization using the reversible addition-fragmentation chain transfer (RAFT) polymerization method. We evaluated its mechanism of action, focusing on two key pathways: temperature-triggered singlet oxygen generation and ferrous ions (Fe2+) release, both of which contribute to ferroptosis. Our results demonstrate that poly(ARTEMA) selectively generates singlet oxygen and Fe2+ due to the endoperoxide crosslinks, leading to cell death in breast cancer cells. We also investigated the anti-cancer potential of poly(ARTEMA) on breast cancer cells with and without a ferroptosis inhibitor. The IC50 values were 125 µM for the MCF-7 cancer cell line and 300 µM for the normal MCF-10 cell line, indicating enhanced toxicity toward cancer cell lines. These findings suggested that poly(ARTEMA) induces ferroptosis in cancer cells and may serve as a promising candidate for cancer therapy with minimal cytotoxicity. To the best of our knowledge, this report may be the first that successfully synthesized poly(ARTEMA) using ART, with its anticancer potential evaluation.https://www.tandfonline.com/doi/10.1080/14686996.2025.2482514Cancer therapyferroptosisartesunateendoperoxideferrous ironpoly(ARTEMA) |
| spellingShingle | Natsumi Ito Ahmed Nabil Koichiro Uto Mitsuhiro Ebara Poly(ARTEMA), a novel artesunate-based polymer induces ferroptosis in breast cancer cells Science and Technology of Advanced Materials Cancer therapy ferroptosis artesunate endoperoxide ferrous iron poly(ARTEMA) |
| title | Poly(ARTEMA), a novel artesunate-based polymer induces ferroptosis in breast cancer cells |
| title_full | Poly(ARTEMA), a novel artesunate-based polymer induces ferroptosis in breast cancer cells |
| title_fullStr | Poly(ARTEMA), a novel artesunate-based polymer induces ferroptosis in breast cancer cells |
| title_full_unstemmed | Poly(ARTEMA), a novel artesunate-based polymer induces ferroptosis in breast cancer cells |
| title_short | Poly(ARTEMA), a novel artesunate-based polymer induces ferroptosis in breast cancer cells |
| title_sort | poly artema a novel artesunate based polymer induces ferroptosis in breast cancer cells |
| topic | Cancer therapy ferroptosis artesunate endoperoxide ferrous iron poly(ARTEMA) |
| url | https://www.tandfonline.com/doi/10.1080/14686996.2025.2482514 |
| work_keys_str_mv | AT natsumiito polyartemaanovelartesunatebasedpolymerinducesferroptosisinbreastcancercells AT ahmednabil polyartemaanovelartesunatebasedpolymerinducesferroptosisinbreastcancercells AT koichirouto polyartemaanovelartesunatebasedpolymerinducesferroptosisinbreastcancercells AT mitsuhiroebara polyartemaanovelartesunatebasedpolymerinducesferroptosisinbreastcancercells |