Identification of a Highly Virulent <i>Verticillium nonalfalfae</i> Strain Vn011 and Expression Analysis of Its Orphan Genes During Potato Inoculation
Potato (<i>Solanum tuberosum</i> L.) is an important food crop and in recent years, Verticillium wilt has become one of the major diseases limiting potato production. To study potato Verticillium wilt, a highly pathogenic strain was isolated from field samples in Heilongjiang. After sequ...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-04-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/14/9/1281 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850135953042046976 |
|---|---|
| author | Mengyuan Wan Xinlong Chen Xiaoxi Yi Yi Fu Yuanliang Jin Dianqiu Lyu |
| author_facet | Mengyuan Wan Xinlong Chen Xiaoxi Yi Yi Fu Yuanliang Jin Dianqiu Lyu |
| author_sort | Mengyuan Wan |
| collection | DOAJ |
| description | Potato (<i>Solanum tuberosum</i> L.) is an important food crop and in recent years, Verticillium wilt has become one of the major diseases limiting potato production. To study potato Verticillium wilt, a highly pathogenic strain was isolated from field samples in Heilongjiang. After sequencing and morphological identification, the strain was confirmed as a host-specialized <i>Verticillium nonalfalfae</i> (<i>V. nonalfalfae</i>), Vn011. The genome analysis revealed 151 orphan genes in Vn011, and comparative transcriptomic analysis before and after potato inoculation showed differential expression of 21 genes, including several encoding low-complexity regions (LCRs) and transmembrane (TM) domains. These domains are known to be involved in pathogen signaling, protein folding, and phase separation processes. This study presents the whole-genome sequence of Vn011, having predicted and analyzed the expression changes of orphan genes during the infection process of <i>V. nonalfalfae</i> in potato, and provides new insights into the pathogenic mechanisms of the pathogen. Further research on these orphan genes will not only enhance the understanding of the evolutionary adaptation of <i>V. nonalfalfae</i>, but may also provide new molecular targets for the control of potato wilt disease. |
| format | Article |
| id | doaj-art-ccd26aae2f6a4ca784214ec8dac78ab8 |
| institution | OA Journals |
| issn | 2223-7747 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj-art-ccd26aae2f6a4ca784214ec8dac78ab82025-08-20T02:31:16ZengMDPI AGPlants2223-77472025-04-01149128110.3390/plants14091281Identification of a Highly Virulent <i>Verticillium nonalfalfae</i> Strain Vn011 and Expression Analysis of Its Orphan Genes During Potato InoculationMengyuan Wan0Xinlong Chen1Xiaoxi Yi2Yi Fu3Yuanliang Jin4Dianqiu Lyu5Integrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City, College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, ChinaIntegrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City, College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, ChinaChongqing Key Laboratory of Biology and Genetic Breeding for Tuber and Root Crops, Chongqing 400715, ChinaChongqing Key Laboratory of Biology and Genetic Breeding for Tuber and Root Crops, Chongqing 400715, ChinaChongqing Key Laboratory of Biology and Genetic Breeding for Tuber and Root Crops, Chongqing 400715, ChinaIntegrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City, College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, ChinaPotato (<i>Solanum tuberosum</i> L.) is an important food crop and in recent years, Verticillium wilt has become one of the major diseases limiting potato production. To study potato Verticillium wilt, a highly pathogenic strain was isolated from field samples in Heilongjiang. After sequencing and morphological identification, the strain was confirmed as a host-specialized <i>Verticillium nonalfalfae</i> (<i>V. nonalfalfae</i>), Vn011. The genome analysis revealed 151 orphan genes in Vn011, and comparative transcriptomic analysis before and after potato inoculation showed differential expression of 21 genes, including several encoding low-complexity regions (LCRs) and transmembrane (TM) domains. These domains are known to be involved in pathogen signaling, protein folding, and phase separation processes. This study presents the whole-genome sequence of Vn011, having predicted and analyzed the expression changes of orphan genes during the infection process of <i>V. nonalfalfae</i> in potato, and provides new insights into the pathogenic mechanisms of the pathogen. Further research on these orphan genes will not only enhance the understanding of the evolutionary adaptation of <i>V. nonalfalfae</i>, but may also provide new molecular targets for the control of potato wilt disease.https://www.mdpi.com/2223-7747/14/9/1281potato (<i>Solanum tuberosum</i> L.)Verticillium wilt<i>Verticillium nonalfalfae</i>orphan genestranscriptome analysis |
| spellingShingle | Mengyuan Wan Xinlong Chen Xiaoxi Yi Yi Fu Yuanliang Jin Dianqiu Lyu Identification of a Highly Virulent <i>Verticillium nonalfalfae</i> Strain Vn011 and Expression Analysis of Its Orphan Genes During Potato Inoculation Plants potato (<i>Solanum tuberosum</i> L.) Verticillium wilt <i>Verticillium nonalfalfae</i> orphan genes transcriptome analysis |
| title | Identification of a Highly Virulent <i>Verticillium nonalfalfae</i> Strain Vn011 and Expression Analysis of Its Orphan Genes During Potato Inoculation |
| title_full | Identification of a Highly Virulent <i>Verticillium nonalfalfae</i> Strain Vn011 and Expression Analysis of Its Orphan Genes During Potato Inoculation |
| title_fullStr | Identification of a Highly Virulent <i>Verticillium nonalfalfae</i> Strain Vn011 and Expression Analysis of Its Orphan Genes During Potato Inoculation |
| title_full_unstemmed | Identification of a Highly Virulent <i>Verticillium nonalfalfae</i> Strain Vn011 and Expression Analysis of Its Orphan Genes During Potato Inoculation |
| title_short | Identification of a Highly Virulent <i>Verticillium nonalfalfae</i> Strain Vn011 and Expression Analysis of Its Orphan Genes During Potato Inoculation |
| title_sort | identification of a highly virulent i verticillium nonalfalfae i strain vn011 and expression analysis of its orphan genes during potato inoculation |
| topic | potato (<i>Solanum tuberosum</i> L.) Verticillium wilt <i>Verticillium nonalfalfae</i> orphan genes transcriptome analysis |
| url | https://www.mdpi.com/2223-7747/14/9/1281 |
| work_keys_str_mv | AT mengyuanwan identificationofahighlyvirulentiverticilliumnonalfalfaeistrainvn011andexpressionanalysisofitsorphangenesduringpotatoinoculation AT xinlongchen identificationofahighlyvirulentiverticilliumnonalfalfaeistrainvn011andexpressionanalysisofitsorphangenesduringpotatoinoculation AT xiaoxiyi identificationofahighlyvirulentiverticilliumnonalfalfaeistrainvn011andexpressionanalysisofitsorphangenesduringpotatoinoculation AT yifu identificationofahighlyvirulentiverticilliumnonalfalfaeistrainvn011andexpressionanalysisofitsorphangenesduringpotatoinoculation AT yuanliangjin identificationofahighlyvirulentiverticilliumnonalfalfaeistrainvn011andexpressionanalysisofitsorphangenesduringpotatoinoculation AT dianqiulyu identificationofahighlyvirulentiverticilliumnonalfalfaeistrainvn011andexpressionanalysisofitsorphangenesduringpotatoinoculation |