High-dose dual therapy for Helicobacter pylori eradication inducing less impact on the gut microbiota
Abstract Background Helicobacter pylori (H. pylori) eradication regimens may have different effects on the gut microbiota. Few studies have analyzed the safety of high-dose dual therapy (HDDT) from a micro-ecological perspective. This study aimed to compare the impact of H. pylori eradication with H...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-01-01
|
| Series: | Gut Pathogens |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13099-025-00682-8 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832571683894984704 |
|---|---|
| author | Jia-Lun Guan Ting-Ting Xu Ya Lin Yan-Shuai Mo Bi-Yu He Ying-Ying Han Ji-Yan Li Su-Hong Xia Ya-Ni Zhou Jia-Zhi Liao Pei-Yuan Li |
| author_facet | Jia-Lun Guan Ting-Ting Xu Ya Lin Yan-Shuai Mo Bi-Yu He Ying-Ying Han Ji-Yan Li Su-Hong Xia Ya-Ni Zhou Jia-Zhi Liao Pei-Yuan Li |
| author_sort | Jia-Lun Guan |
| collection | DOAJ |
| description | Abstract Background Helicobacter pylori (H. pylori) eradication regimens may have different effects on the gut microbiota. Few studies have analyzed the safety of high-dose dual therapy (HDDT) from a micro-ecological perspective. This study aimed to compare the impact of H. pylori eradication with HDDT and bismuth quadruple therapy (BQT) on gut microbiota. Patients and methods H. Pylori-infected treatment-naive patients were recruited and screened from September 2023 to April 2024 and randomly assigned to the HDDT group (esomeprazole 20 mg, amoxicillin 750 mg, qid, 14 days) or BQT group (esomeprazole 20 mg, amoxicillin 1000 mg, clarithromycin 500 mg, and bismuth potassium citrate 600 mg, bid, 14 days). Fresh stool specimens were collected and stored before treatment and at week 2 and week 8 after treatment. The diversity and composition of the gut microbiota were compared and analyzed in both groups using 16 S rRNA gene sequencing. Results Forty-nine H. pylori positive patients were enrolled and randomly assigned to either the HDDT (n = 24) or the BQT group (n = 25) group. Compared with baseline, alpha and beta diversities significantly changed at week 2 after receiving BQT and did not recover fully at week 8. However, in the HDDT group, the diversities at week 2 changed mildly without statistical significance, compared to baseline. Additionally, a greater number of species had alterations in their abundances in the BQT group compared to the HDDT group at week 2. However, the abundances of these species were restored to their previous levels at week 8 in both the HDDT and BQT groups. Conclusions Compared to BQT, HDDT exerted less impact on the diversity and composition of the gut microbiota. Clinical trial registration ChiCTR2100053268. |
| format | Article |
| id | doaj-art-ccd14af0342c47a98cfc8d00df7f83e3 |
| institution | Kabale University |
| issn | 1757-4749 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Gut Pathogens |
| spelling | doaj-art-ccd14af0342c47a98cfc8d00df7f83e32025-02-02T12:26:00ZengBMCGut Pathogens1757-47492025-01-0117111410.1186/s13099-025-00682-8High-dose dual therapy for Helicobacter pylori eradication inducing less impact on the gut microbiotaJia-Lun Guan0Ting-Ting Xu1Ya Lin2Yan-Shuai Mo3Bi-Yu He4Ying-Ying Han5Ji-Yan Li6Su-Hong Xia7Ya-Ni Zhou8Jia-Zhi Liao9Pei-Yuan Li10Division of Gastroenterology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Gastroenterology, Wenchang People’s HospitalDepartment of Gastroenterology, Wenchang People’s HospitalDepartment of Anesthesiology, Wenchang People’s HospitalDepartment of Gastroenterology, Wenchang People’s HospitalDivision of Gastroenterology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and TechnologyDivision of Gastroenterology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and TechnologyDivision of Gastroenterology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and TechnologyDivision of Gastroenterology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and TechnologyDivision of Gastroenterology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and TechnologyDivision of Gastroenterology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and TechnologyAbstract Background Helicobacter pylori (H. pylori) eradication regimens may have different effects on the gut microbiota. Few studies have analyzed the safety of high-dose dual therapy (HDDT) from a micro-ecological perspective. This study aimed to compare the impact of H. pylori eradication with HDDT and bismuth quadruple therapy (BQT) on gut microbiota. Patients and methods H. Pylori-infected treatment-naive patients were recruited and screened from September 2023 to April 2024 and randomly assigned to the HDDT group (esomeprazole 20 mg, amoxicillin 750 mg, qid, 14 days) or BQT group (esomeprazole 20 mg, amoxicillin 1000 mg, clarithromycin 500 mg, and bismuth potassium citrate 600 mg, bid, 14 days). Fresh stool specimens were collected and stored before treatment and at week 2 and week 8 after treatment. The diversity and composition of the gut microbiota were compared and analyzed in both groups using 16 S rRNA gene sequencing. Results Forty-nine H. pylori positive patients were enrolled and randomly assigned to either the HDDT (n = 24) or the BQT group (n = 25) group. Compared with baseline, alpha and beta diversities significantly changed at week 2 after receiving BQT and did not recover fully at week 8. However, in the HDDT group, the diversities at week 2 changed mildly without statistical significance, compared to baseline. Additionally, a greater number of species had alterations in their abundances in the BQT group compared to the HDDT group at week 2. However, the abundances of these species were restored to their previous levels at week 8 in both the HDDT and BQT groups. Conclusions Compared to BQT, HDDT exerted less impact on the diversity and composition of the gut microbiota. Clinical trial registration ChiCTR2100053268.https://doi.org/10.1186/s13099-025-00682-8Helicobacter pyloriGut microbiotaHigh-dose dual therapyBismuth quadruple therapy |
| spellingShingle | Jia-Lun Guan Ting-Ting Xu Ya Lin Yan-Shuai Mo Bi-Yu He Ying-Ying Han Ji-Yan Li Su-Hong Xia Ya-Ni Zhou Jia-Zhi Liao Pei-Yuan Li High-dose dual therapy for Helicobacter pylori eradication inducing less impact on the gut microbiota Gut Pathogens Helicobacter pylori Gut microbiota High-dose dual therapy Bismuth quadruple therapy |
| title | High-dose dual therapy for Helicobacter pylori eradication inducing less impact on the gut microbiota |
| title_full | High-dose dual therapy for Helicobacter pylori eradication inducing less impact on the gut microbiota |
| title_fullStr | High-dose dual therapy for Helicobacter pylori eradication inducing less impact on the gut microbiota |
| title_full_unstemmed | High-dose dual therapy for Helicobacter pylori eradication inducing less impact on the gut microbiota |
| title_short | High-dose dual therapy for Helicobacter pylori eradication inducing less impact on the gut microbiota |
| title_sort | high dose dual therapy for helicobacter pylori eradication inducing less impact on the gut microbiota |
| topic | Helicobacter pylori Gut microbiota High-dose dual therapy Bismuth quadruple therapy |
| url | https://doi.org/10.1186/s13099-025-00682-8 |
| work_keys_str_mv | AT jialunguan highdosedualtherapyforhelicobacterpylorieradicationinducinglessimpactonthegutmicrobiota AT tingtingxu highdosedualtherapyforhelicobacterpylorieradicationinducinglessimpactonthegutmicrobiota AT yalin highdosedualtherapyforhelicobacterpylorieradicationinducinglessimpactonthegutmicrobiota AT yanshuaimo highdosedualtherapyforhelicobacterpylorieradicationinducinglessimpactonthegutmicrobiota AT biyuhe highdosedualtherapyforhelicobacterpylorieradicationinducinglessimpactonthegutmicrobiota AT yingyinghan highdosedualtherapyforhelicobacterpylorieradicationinducinglessimpactonthegutmicrobiota AT jiyanli highdosedualtherapyforhelicobacterpylorieradicationinducinglessimpactonthegutmicrobiota AT suhongxia highdosedualtherapyforhelicobacterpylorieradicationinducinglessimpactonthegutmicrobiota AT yanizhou highdosedualtherapyforhelicobacterpylorieradicationinducinglessimpactonthegutmicrobiota AT jiazhiliao highdosedualtherapyforhelicobacterpylorieradicationinducinglessimpactonthegutmicrobiota AT peiyuanli highdosedualtherapyforhelicobacterpylorieradicationinducinglessimpactonthegutmicrobiota |