Effects of a single application of hydrolyzed fish collagen on dermal protein expression and tissue architecture in human skin models

Abstract The use of collagen as an active ingredient in cosmetic products is becoming increasingly common. Recently, scientific interest has been focused on fish collagen, which is similar to human collagen and can be extracted from fish by-products. This study evaluates the cosmetic potential of Hy...

Full description

Saved in:
Bibliographic Details
Main Authors: Lorenzo Dondero, Giulia De Negri Atanasio, Erica Lertora, Francesca Tardanico, Ilaria Demori, Pietro Storace, Giorgia Allaria, Francesca Rispo, Federica Robino, Elisabetta Perata, Matteo Zanotti-Russo, Sara Ferrando, Elena Grasselli
Format: Article
Language:English
Published: Nature Portfolio 2025-08-01
Series:Scientific Reports
Subjects:
Online Access:https://doi.org/10.1038/s41598-025-11372-5
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849333569796702208
author Lorenzo Dondero
Giulia De Negri Atanasio
Erica Lertora
Francesca Tardanico
Ilaria Demori
Pietro Storace
Giorgia Allaria
Francesca Rispo
Federica Robino
Elisabetta Perata
Matteo Zanotti-Russo
Sara Ferrando
Elena Grasselli
author_facet Lorenzo Dondero
Giulia De Negri Atanasio
Erica Lertora
Francesca Tardanico
Ilaria Demori
Pietro Storace
Giorgia Allaria
Francesca Rispo
Federica Robino
Elisabetta Perata
Matteo Zanotti-Russo
Sara Ferrando
Elena Grasselli
author_sort Lorenzo Dondero
collection DOAJ
description Abstract The use of collagen as an active ingredient in cosmetic products is becoming increasingly common. Recently, scientific interest has been focused on fish collagen, which is similar to human collagen and can be extracted from fish by-products. This study evaluates the cosmetic potential of Hydrolyzed Fish Collagen (HFC), testing it both as an aqueous solution (S-HFC) and as a formulation in cosmetic products (F-HFC) containing the same amount of HFC. The effects of S-HFC and F-HFC were evaluated on 2D (endothelial and keratinocyte cells) and 3D (EpiDerm and EpiDerm-FT) skin models. The results demonstrated that HFC, as an active ingredient, shows promising effects on skin physiology, including increased cell viability, enhanced wound healing, and an anti-wrinkle effect.
format Article
id doaj-art-ccca3817ac694056bafe385ebba7b1bb
institution Kabale University
issn 2045-2322
language English
publishDate 2025-08-01
publisher Nature Portfolio
record_format Article
series Scientific Reports
spelling doaj-art-ccca3817ac694056bafe385ebba7b1bb2025-08-20T03:45:48ZengNature PortfolioScientific Reports2045-23222025-08-0115111310.1038/s41598-025-11372-5Effects of a single application of hydrolyzed fish collagen on dermal protein expression and tissue architecture in human skin modelsLorenzo Dondero0Giulia De Negri Atanasio1Erica Lertora2Francesca Tardanico3Ilaria Demori4Pietro Storace5Giorgia Allaria6Francesca Rispo7Federica Robino8Elisabetta Perata9Matteo Zanotti-Russo10Sara Ferrando11Elena Grasselli12Department of Earth, Environment and Life Science, University of GenoaDepartment of Earth, Environment and Life Science, University of GenoaDepartment of Earth, Environment and Life Science, University of GenoaDepartment of Earth, Environment and Life Science, University of GenoaDepartment of Pharmacy, University of GenoaDepartment of Earth, Environment and Life Science, University of GenoaDepartment of Earth, Environment and Life Science, University of GenoaDepartment of Earth, Environment and Life Science, University of GenoaDepartment of Earth, Environment and Life Science, University of GenoaDepartment of Earth, Environment and Life Science, University of GenoaAngel ConsultingDepartment of Earth, Environment and Life Science, University of GenoaDepartment of Earth, Environment and Life Science, University of GenoaAbstract The use of collagen as an active ingredient in cosmetic products is becoming increasingly common. Recently, scientific interest has been focused on fish collagen, which is similar to human collagen and can be extracted from fish by-products. This study evaluates the cosmetic potential of Hydrolyzed Fish Collagen (HFC), testing it both as an aqueous solution (S-HFC) and as a formulation in cosmetic products (F-HFC) containing the same amount of HFC. The effects of S-HFC and F-HFC were evaluated on 2D (endothelial and keratinocyte cells) and 3D (EpiDerm and EpiDerm-FT) skin models. The results demonstrated that HFC, as an active ingredient, shows promising effects on skin physiology, including increased cell viability, enhanced wound healing, and an anti-wrinkle effect.https://doi.org/10.1038/s41598-025-11372-5Hydrolyzed fish collagenCircular economySkin ageingNew approach methodologiesNext generation risk assessment
spellingShingle Lorenzo Dondero
Giulia De Negri Atanasio
Erica Lertora
Francesca Tardanico
Ilaria Demori
Pietro Storace
Giorgia Allaria
Francesca Rispo
Federica Robino
Elisabetta Perata
Matteo Zanotti-Russo
Sara Ferrando
Elena Grasselli
Effects of a single application of hydrolyzed fish collagen on dermal protein expression and tissue architecture in human skin models
Scientific Reports
Hydrolyzed fish collagen
Circular economy
Skin ageing
New approach methodologies
Next generation risk assessment
title Effects of a single application of hydrolyzed fish collagen on dermal protein expression and tissue architecture in human skin models
title_full Effects of a single application of hydrolyzed fish collagen on dermal protein expression and tissue architecture in human skin models
title_fullStr Effects of a single application of hydrolyzed fish collagen on dermal protein expression and tissue architecture in human skin models
title_full_unstemmed Effects of a single application of hydrolyzed fish collagen on dermal protein expression and tissue architecture in human skin models
title_short Effects of a single application of hydrolyzed fish collagen on dermal protein expression and tissue architecture in human skin models
title_sort effects of a single application of hydrolyzed fish collagen on dermal protein expression and tissue architecture in human skin models
topic Hydrolyzed fish collagen
Circular economy
Skin ageing
New approach methodologies
Next generation risk assessment
url https://doi.org/10.1038/s41598-025-11372-5
work_keys_str_mv AT lorenzodondero effectsofasingleapplicationofhydrolyzedfishcollagenondermalproteinexpressionandtissuearchitectureinhumanskinmodels
AT giuliadenegriatanasio effectsofasingleapplicationofhydrolyzedfishcollagenondermalproteinexpressionandtissuearchitectureinhumanskinmodels
AT ericalertora effectsofasingleapplicationofhydrolyzedfishcollagenondermalproteinexpressionandtissuearchitectureinhumanskinmodels
AT francescatardanico effectsofasingleapplicationofhydrolyzedfishcollagenondermalproteinexpressionandtissuearchitectureinhumanskinmodels
AT ilariademori effectsofasingleapplicationofhydrolyzedfishcollagenondermalproteinexpressionandtissuearchitectureinhumanskinmodels
AT pietrostorace effectsofasingleapplicationofhydrolyzedfishcollagenondermalproteinexpressionandtissuearchitectureinhumanskinmodels
AT giorgiaallaria effectsofasingleapplicationofhydrolyzedfishcollagenondermalproteinexpressionandtissuearchitectureinhumanskinmodels
AT francescarispo effectsofasingleapplicationofhydrolyzedfishcollagenondermalproteinexpressionandtissuearchitectureinhumanskinmodels
AT federicarobino effectsofasingleapplicationofhydrolyzedfishcollagenondermalproteinexpressionandtissuearchitectureinhumanskinmodels
AT elisabettaperata effectsofasingleapplicationofhydrolyzedfishcollagenondermalproteinexpressionandtissuearchitectureinhumanskinmodels
AT matteozanottirusso effectsofasingleapplicationofhydrolyzedfishcollagenondermalproteinexpressionandtissuearchitectureinhumanskinmodels
AT saraferrando effectsofasingleapplicationofhydrolyzedfishcollagenondermalproteinexpressionandtissuearchitectureinhumanskinmodels
AT elenagrasselli effectsofasingleapplicationofhydrolyzedfishcollagenondermalproteinexpressionandtissuearchitectureinhumanskinmodels