Effect of major royal jelly proteins (MRJPs) on proliferation activity of Chang's liver cell line and their mechanism of action
Major royal jelly proteins (MRJPs) are the soluble proteins in royal jelly, which account for 82%-90% of total royal jelly proteins. MRJPs are consisted of ten members, i. e. MRJP1-MRJP9 and MRJPψ, whose biological functions include enhancing cell proliferation, inducing differentiation, modulating...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2015-01-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2014.03.231 |
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| Summary: | Major royal jelly proteins (MRJPs) are the soluble proteins in royal jelly, which account for 82%-90% of total royal jelly proteins. MRJPs are consisted of ten members, i. e. MRJP1-MRJP9 and MRJPψ, whose biological functions include enhancing cell proliferation, inducing differentiation, modulating immune responses, accelerating wound healing, etc. MRJP1 with 57 ku in molecular mass was found possessing proliferation-promoting activities, enhancing proliferation of primary cultured rat hepatocytes and increasing albumin production in the absence of serum. MRJPs could significantly stimulate the proliferation and growth of endothelial progenitor cells (EPC). The recombinant MRJP1 could significantly stimulate growth of Tn-5B-4 cell from Trichoplusia ni, and affect cell shape and adhesion to the substrate. Similar activities were also found in normal human neonatal skin fibroblasts NB1 RGB cell, MC3T3-E1 cell and Jurkat cell, etc. All these findings showed the potential of MRJPs in cell culture as cell growth factor. Besides, it seemed that MRJP1 could promote liver regeneration and might have a cytoprotective action on hepatocytes. Here, we used Chang's liver cell from human as object to observe the proliferation action of MRJPs on the cell line, and to explore the potential of MRJPs substituting fetal bovine serum (FBS) as growth factor; the active mechanism of MRJPs was also investigated.MRJPs were extracted by centrifuging from fresh royal jelly. The optimum concentration was selected by MTT assaying of the proliferation rates of the cell (PRC) cultured with serum-free mediums containing 0.05, 0.1, 0.2, 0.3 and 0.5 mg/mL of MRJPs at 5th day. The possibility of MRJPs with optimum concentration (0.5 mg/mL) to replace FBS was investigated via comparison of the effects on PRC at the 2nd day, 5th day and 8th day in both serum-free and serum-existing mediums with a positive control containing 10% FBS and a negative control containing 10% phosphate buffer solution (PBS). It was showed that MRJPs could only be used to culture the cell line when it was mixed with FBS. Then, the effects of different MRJPs/FBS (M/F) ratios (0/100, 30/70, 60/40, 90/10, 100/0) on PRC at the 2nd day, 5th day and 8th day were investigated, respectively. It was shown that the M/F ratios with 30/70 and 60/40 were better for PRC of the cell line in relative to the complete medium with 10% FBS (M/F=0/100). The cell cycle distributions of the cell line cultured with different M/F ratios (0/100, 30/70, 60/40, 100/0) were detected by flow cytometry. It was shown that the proliferation index (PI), S phase and G<sub>0</sub>/G<sub>1</sub> phase of the cell in the medium with M/F of 60/40 at the 5th day were not significantly different from the cell in complete medium (P>0.05), indicating that MRJPs might promote DNA synthesizing in S phase or RNA and protein synthesizing in G<sub>0</sub>/G<sub>1</sub> phase.In conclusion, MRJPs mixed with FBS are suggested partially to replace FBS to culture Chang's liver cell line in practice. |
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| ISSN: | 1008-9209 2097-5155 |