Prenylated flavonoid morusin protects against TNBS-induced colitis in rats.
Morusin is a prenylated flavonoid isolated from the root bark of Morus alba. Many studies have shown the ability of flavonoids to act as anti-inflammatory agents. The aim of this study was to evaluate the effect of morusin on experimentally colitis induced by 2,4,6-trinitrobenzensulfonic acid in Wis...
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0182464&type=printable |
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| author | Zora Vochyánová Marie Pokorná Dominik Rotrekl Václav Smékal Petr Fictum Pavel Suchý Jan Gajdziok Karel Šmejkal Jan Hošek |
| author_facet | Zora Vochyánová Marie Pokorná Dominik Rotrekl Václav Smékal Petr Fictum Pavel Suchý Jan Gajdziok Karel Šmejkal Jan Hošek |
| author_sort | Zora Vochyánová |
| collection | DOAJ |
| description | Morusin is a prenylated flavonoid isolated from the root bark of Morus alba. Many studies have shown the ability of flavonoids to act as anti-inflammatory agents. The aim of this study was to evaluate the effect of morusin on experimentally colitis induced by 2,4,6-trinitrobenzensulfonic acid in Wistar rats and to compare it with sulfasalazine, a drug conventionally used in the treatment of inflammatory bowel disease. Morusin was administered by gavage at doses of 12.5, 25, or 50 mg/kg/day for five days. The colonic tissue was evaluated macroscopically, histologically, and by performing immunodetection and zymographic analysis to determine the levels of antioxidant enzymes [superoxide dismutase (SOD) and catalase (CAT)], interleukin (IL)-1β, and transforming growth factor (TGF)-β1 and the activities of matrix metalloproteinases (MMP) 2 and 9. The tissue damage scores were significantly reduced with increasing dose of morusin, however efficacy was not demonstrated at the highest dose. At the dose of 12.5 mg/kg, morusin exerted therapeutic effectivity similar to that of sulfasalazine (50 mg/kg). This was associated with significant reduction of TGF-β1 levels and MMP2 and MMP9 activities, and slight reduction of IL-1β. Our results suggest that morusin possesses therapeutic potential for the treatment of chronic inflammatory diseases. |
| format | Article |
| id | doaj-art-ccb5a6bd8a21444b9f164f3c9c81d8a5 |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj-art-ccb5a6bd8a21444b9f164f3c9c81d8a52025-08-20T02:46:04ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01128e018246410.1371/journal.pone.0182464Prenylated flavonoid morusin protects against TNBS-induced colitis in rats.Zora VochyánováMarie PokornáDominik RotreklVáclav SmékalPetr FictumPavel SuchýJan GajdziokKarel ŠmejkalJan HošekMorusin is a prenylated flavonoid isolated from the root bark of Morus alba. Many studies have shown the ability of flavonoids to act as anti-inflammatory agents. The aim of this study was to evaluate the effect of morusin on experimentally colitis induced by 2,4,6-trinitrobenzensulfonic acid in Wistar rats and to compare it with sulfasalazine, a drug conventionally used in the treatment of inflammatory bowel disease. Morusin was administered by gavage at doses of 12.5, 25, or 50 mg/kg/day for five days. The colonic tissue was evaluated macroscopically, histologically, and by performing immunodetection and zymographic analysis to determine the levels of antioxidant enzymes [superoxide dismutase (SOD) and catalase (CAT)], interleukin (IL)-1β, and transforming growth factor (TGF)-β1 and the activities of matrix metalloproteinases (MMP) 2 and 9. The tissue damage scores were significantly reduced with increasing dose of morusin, however efficacy was not demonstrated at the highest dose. At the dose of 12.5 mg/kg, morusin exerted therapeutic effectivity similar to that of sulfasalazine (50 mg/kg). This was associated with significant reduction of TGF-β1 levels and MMP2 and MMP9 activities, and slight reduction of IL-1β. Our results suggest that morusin possesses therapeutic potential for the treatment of chronic inflammatory diseases.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0182464&type=printable |
| spellingShingle | Zora Vochyánová Marie Pokorná Dominik Rotrekl Václav Smékal Petr Fictum Pavel Suchý Jan Gajdziok Karel Šmejkal Jan Hošek Prenylated flavonoid morusin protects against TNBS-induced colitis in rats. PLoS ONE |
| title | Prenylated flavonoid morusin protects against TNBS-induced colitis in rats. |
| title_full | Prenylated flavonoid morusin protects against TNBS-induced colitis in rats. |
| title_fullStr | Prenylated flavonoid morusin protects against TNBS-induced colitis in rats. |
| title_full_unstemmed | Prenylated flavonoid morusin protects against TNBS-induced colitis in rats. |
| title_short | Prenylated flavonoid morusin protects against TNBS-induced colitis in rats. |
| title_sort | prenylated flavonoid morusin protects against tnbs induced colitis in rats |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0182464&type=printable |
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