The gene expression of caspase 3, matrix metalloproteinase-9, tissue inhibitor of metalloproteinases-1, and cathepsins S and K in patients with osteoarthritis requiring large joint replacement
Osteoarthritis (OA) is a chronic rheumatic disease that is characterized by pain and articular cartilage degradation. Pain in OA is a main clinical symptom that limits working capacity and is one of the indications for joint replacement. However, 10-40% of patients with OA also continue to experienc...
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2020-05-01
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| Series: | Современная ревматология |
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| Online Access: | https://mrj.ima-press.net/mrj/article/view/1016 |
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| author | E. V. Chetina K. E. Glemba G. A. Markova S. A. Makarov |
| author_facet | E. V. Chetina K. E. Glemba G. A. Markova S. A. Makarov |
| author_sort | E. V. Chetina |
| collection | DOAJ |
| description | Osteoarthritis (OA) is a chronic rheumatic disease that is characterized by pain and articular cartilage degradation. Pain in OA is a main clinical symptom that limits working capacity and is one of the indications for joint replacement. However, 10-40% of patients with OA also continue to experience painful sensations after surgery.Objective: to develop a method for searching for biomarkers to predict the dynamics of pain in the postoperative period and to determine the feasibility of arthroplasty on the basis of a retrospective analysis of relative blood gene expression prior to surgery.Patients and methods. The investigators tested the blood taken from 53 OA patients (mean age, 56.5±8.9 years) before knee arthroplasty and from 26 healthy donors (mean age, 55±8.3 years). Total RNA was isolated from blood and after reverse transcription into complementary DNA was used to measure the level of relative gene expression in real-time polymerase chain reaction.Results and discussion. A retrospective analysis of the expression of genes associated with central sensitization in 53 patients with OA before arthroplasty showed that the data on the expression of tumor necrosis factor-α, interleukin-1β, cyclooxygenase-2, and transforming growth factor β1 were uninformative due to their high blood expression in all the patients. The high gene expression of cathepsin S (in 17% of the patients) and cathepsin K (in 21%) and the low gene expression of tissue inhibitor of metalloproteinases 1 (TIMP-1) (in 31%) may indicate that postoperative pain can be persistent. In contrast, no post-arthroplasty pain can be expected in 43% OA patients with low caspase 3 expression and in 23% of those with low MMP-9 one.Conclusion. Analysis of pre-arthroplasty blood gene expression in patients with OA seems to be a promising approach to predicting the dynamics of pain after surgical treatment. |
| format | Article |
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| institution | DOAJ |
| issn | 1996-7012 2310-158X |
| language | Russian |
| publishDate | 2020-05-01 |
| publisher | IMA-PRESS LLC |
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| series | Современная ревматология |
| spelling | doaj-art-ccb2ca98927c4636bfc7b9fc46de62692025-08-20T03:20:58ZrusIMA-PRESS LLCСовременная ревматология1996-70122310-158X2020-05-01142525610.14412/1996-7012-2020-2-52-562257The gene expression of caspase 3, matrix metalloproteinase-9, tissue inhibitor of metalloproteinases-1, and cathepsins S and K in patients with osteoarthritis requiring large joint replacementE. V. Chetina0K. E. Glemba1G. A. Markova2S. A. Makarov3V.A. Nasonova Research Institute of RheumatologyV.A. Nasonova Research Institute of RheumatologyV.A. Nasonova Research Institute of RheumatologyV.A. Nasonova Research Institute of RheumatologyOsteoarthritis (OA) is a chronic rheumatic disease that is characterized by pain and articular cartilage degradation. Pain in OA is a main clinical symptom that limits working capacity and is one of the indications for joint replacement. However, 10-40% of patients with OA also continue to experience painful sensations after surgery.Objective: to develop a method for searching for biomarkers to predict the dynamics of pain in the postoperative period and to determine the feasibility of arthroplasty on the basis of a retrospective analysis of relative blood gene expression prior to surgery.Patients and methods. The investigators tested the blood taken from 53 OA patients (mean age, 56.5±8.9 years) before knee arthroplasty and from 26 healthy donors (mean age, 55±8.3 years). Total RNA was isolated from blood and after reverse transcription into complementary DNA was used to measure the level of relative gene expression in real-time polymerase chain reaction.Results and discussion. A retrospective analysis of the expression of genes associated with central sensitization in 53 patients with OA before arthroplasty showed that the data on the expression of tumor necrosis factor-α, interleukin-1β, cyclooxygenase-2, and transforming growth factor β1 were uninformative due to their high blood expression in all the patients. The high gene expression of cathepsin S (in 17% of the patients) and cathepsin K (in 21%) and the low gene expression of tissue inhibitor of metalloproteinases 1 (TIMP-1) (in 31%) may indicate that postoperative pain can be persistent. In contrast, no post-arthroplasty pain can be expected in 43% OA patients with low caspase 3 expression and in 23% of those with low MMP-9 one.Conclusion. Analysis of pre-arthroplasty blood gene expression in patients with OA seems to be a promising approach to predicting the dynamics of pain after surgical treatment.https://mrj.ima-press.net/mrj/article/view/1016osteoarthritispaingene expressionperipheral bloodprediction of arthroplasty outcome |
| spellingShingle | E. V. Chetina K. E. Glemba G. A. Markova S. A. Makarov The gene expression of caspase 3, matrix metalloproteinase-9, tissue inhibitor of metalloproteinases-1, and cathepsins S and K in patients with osteoarthritis requiring large joint replacement Современная ревматология osteoarthritis pain gene expression peripheral blood prediction of arthroplasty outcome |
| title | The gene expression of caspase 3, matrix metalloproteinase-9, tissue inhibitor of metalloproteinases-1, and cathepsins S and K in patients with osteoarthritis requiring large joint replacement |
| title_full | The gene expression of caspase 3, matrix metalloproteinase-9, tissue inhibitor of metalloproteinases-1, and cathepsins S and K in patients with osteoarthritis requiring large joint replacement |
| title_fullStr | The gene expression of caspase 3, matrix metalloproteinase-9, tissue inhibitor of metalloproteinases-1, and cathepsins S and K in patients with osteoarthritis requiring large joint replacement |
| title_full_unstemmed | The gene expression of caspase 3, matrix metalloproteinase-9, tissue inhibitor of metalloproteinases-1, and cathepsins S and K in patients with osteoarthritis requiring large joint replacement |
| title_short | The gene expression of caspase 3, matrix metalloproteinase-9, tissue inhibitor of metalloproteinases-1, and cathepsins S and K in patients with osteoarthritis requiring large joint replacement |
| title_sort | gene expression of caspase 3 matrix metalloproteinase 9 tissue inhibitor of metalloproteinases 1 and cathepsins s and k in patients with osteoarthritis requiring large joint replacement |
| topic | osteoarthritis pain gene expression peripheral blood prediction of arthroplasty outcome |
| url | https://mrj.ima-press.net/mrj/article/view/1016 |
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