Quantitative intra-Golgi transport and organization data suggest the stable compartment nature of the Golgi
How the intra-Golgi secretory transport works remains a mystery. The cisternal progression and the stable compartment models have been proposed and are under debate. Classic cisternal progression model posits that both the intra-Golgi transport and Golgi exit of secretory cargos should occur at a co...
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eLife Sciences Publications Ltd
2025-07-01
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| Online Access: | https://elifesciences.org/articles/98582 |
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| author | Hieng Chiong Tie Haiyun Wang Divyanshu Mahajan Hilbert Yuen In Lam Xiuping Sun Bing Chen Yuguang Mu Lei Lu |
| author_facet | Hieng Chiong Tie Haiyun Wang Divyanshu Mahajan Hilbert Yuen In Lam Xiuping Sun Bing Chen Yuguang Mu Lei Lu |
| author_sort | Hieng Chiong Tie |
| collection | DOAJ |
| description | How the intra-Golgi secretory transport works remains a mystery. The cisternal progression and the stable compartment models have been proposed and are under debate. Classic cisternal progression model posits that both the intra-Golgi transport and Golgi exit of secretory cargos should occur at a constant velocity dictated by the cisternal progression; furthermore, COPI-mediated intra-Golgi retrograde transport is essential for maintaining the Golgi organization. Leveraging our recently developed Golgi imaging tools in nocodazole-induced Golgi ministacks, we found that the intra-Golgi transport velocity of a secretory cargo decreases during their transition from the cis to the trans-side of the Golgi, and different cargos exhibit distinct velocities even within the same cisternae. We observed a vast variation in the Golgi residence times of different cargos. Remarkably, truncation of the luminal domain causes the Golgi residence time of Tac — a standard transmembrane secretory cargo without intra-Golgi recycling signals — to extend from 16 min to a notable 3.4 hr. Additionally, when COPI-mediated intra-Golgi retrograde transport was inhibited by brefeldin A, we found that nocodazole-induced Golgi can remain stacked for over 30–60 min. Therefore, our findings challenge the classical cisternal progression model and suggest the stable compartment nature of the Golgi. |
| format | Article |
| id | doaj-art-ccb1dd8543994fbcbfdc508c5a118e21 |
| institution | DOAJ |
| issn | 2050-084X |
| language | English |
| publishDate | 2025-07-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj-art-ccb1dd8543994fbcbfdc508c5a118e212025-08-20T03:17:44ZengeLife Sciences Publications LtdeLife2050-084X2025-07-011310.7554/eLife.98582Quantitative intra-Golgi transport and organization data suggest the stable compartment nature of the GolgiHieng Chiong Tie0https://orcid.org/0000-0003-2738-8685Haiyun Wang1https://orcid.org/0009-0003-5963-2029Divyanshu Mahajan2https://orcid.org/0000-0001-5820-6763Hilbert Yuen In Lam3https://orcid.org/0000-0001-6129-2703Xiuping Sun4Bing Chen5Yuguang Mu6Lei Lu7https://orcid.org/0000-0002-8192-1471School of Biological Sciences, Nanyang Technological University, Singapore, Singapore; Medisix Therapeutics, Singapore, SingaporeSchool of Biological Sciences, Nanyang Technological University, Singapore, Singapore; Medisix Therapeutics, Singapore, SingaporeSchool of Biological Sciences, Nanyang Technological University, Singapore, SingaporeSchool of Biological Sciences, Nanyang Technological University, Singapore, SingaporeSchool of Biological Sciences, Nanyang Technological University, Singapore, SingaporeSchool of Biological Sciences, Nanyang Technological University, Singapore, SingaporeSchool of Biological Sciences, Nanyang Technological University, Singapore, SingaporeSchool of Biological Sciences, Nanyang Technological University, Singapore, SingaporeHow the intra-Golgi secretory transport works remains a mystery. The cisternal progression and the stable compartment models have been proposed and are under debate. Classic cisternal progression model posits that both the intra-Golgi transport and Golgi exit of secretory cargos should occur at a constant velocity dictated by the cisternal progression; furthermore, COPI-mediated intra-Golgi retrograde transport is essential for maintaining the Golgi organization. Leveraging our recently developed Golgi imaging tools in nocodazole-induced Golgi ministacks, we found that the intra-Golgi transport velocity of a secretory cargo decreases during their transition from the cis to the trans-side of the Golgi, and different cargos exhibit distinct velocities even within the same cisternae. We observed a vast variation in the Golgi residence times of different cargos. Remarkably, truncation of the luminal domain causes the Golgi residence time of Tac — a standard transmembrane secretory cargo without intra-Golgi recycling signals — to extend from 16 min to a notable 3.4 hr. Additionally, when COPI-mediated intra-Golgi retrograde transport was inhibited by brefeldin A, we found that nocodazole-induced Golgi can remain stacked for over 30–60 min. Therefore, our findings challenge the classical cisternal progression model and suggest the stable compartment nature of the Golgi.https://elifesciences.org/articles/98582Golgimembrane traffickingintra-Golgi transportcisternal progression modelstable compartment model |
| spellingShingle | Hieng Chiong Tie Haiyun Wang Divyanshu Mahajan Hilbert Yuen In Lam Xiuping Sun Bing Chen Yuguang Mu Lei Lu Quantitative intra-Golgi transport and organization data suggest the stable compartment nature of the Golgi eLife Golgi membrane trafficking intra-Golgi transport cisternal progression model stable compartment model |
| title | Quantitative intra-Golgi transport and organization data suggest the stable compartment nature of the Golgi |
| title_full | Quantitative intra-Golgi transport and organization data suggest the stable compartment nature of the Golgi |
| title_fullStr | Quantitative intra-Golgi transport and organization data suggest the stable compartment nature of the Golgi |
| title_full_unstemmed | Quantitative intra-Golgi transport and organization data suggest the stable compartment nature of the Golgi |
| title_short | Quantitative intra-Golgi transport and organization data suggest the stable compartment nature of the Golgi |
| title_sort | quantitative intra golgi transport and organization data suggest the stable compartment nature of the golgi |
| topic | Golgi membrane trafficking intra-Golgi transport cisternal progression model stable compartment model |
| url | https://elifesciences.org/articles/98582 |
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