Epigallocatechin Gallate in <i>Camellia sinensis</i> Ameliorates Skin Aging by Reducing Mitochondrial ROS Production

<b>Background:</b> Reactive oxygen species (ROS) generated by mitochondrial dysfunction damage cellular organelles and contribute to skin aging. Therefore, strategies to reduce mitochondrial ROS production are considered important for alleviating skin aging, but no effective methods have...

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Main Authors: Ji Ho Park, Eun Young Jeong, Ye Hyang Kim, So Yoon Cha, Ha Yeon Kim, Yeon Kyung Nam, Jin Seong Park, So Yeon Kim, Yoo Jin Lee, Jee Hee Yoon, Byeonghyeon So, Duyeol Kim, Minseon Kim, Youngjoo Byun, Yun Haeng Lee, Song Seok Shin, Joon Tae Park
Format: Article
Language:English
Published: MDPI AG 2025-04-01
Series:Pharmaceuticals
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Online Access:https://www.mdpi.com/1424-8247/18/5/612
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author Ji Ho Park
Eun Young Jeong
Ye Hyang Kim
So Yoon Cha
Ha Yeon Kim
Yeon Kyung Nam
Jin Seong Park
So Yeon Kim
Yoo Jin Lee
Jee Hee Yoon
Byeonghyeon So
Duyeol Kim
Minseon Kim
Youngjoo Byun
Yun Haeng Lee
Song Seok Shin
Joon Tae Park
author_facet Ji Ho Park
Eun Young Jeong
Ye Hyang Kim
So Yoon Cha
Ha Yeon Kim
Yeon Kyung Nam
Jin Seong Park
So Yeon Kim
Yoo Jin Lee
Jee Hee Yoon
Byeonghyeon So
Duyeol Kim
Minseon Kim
Youngjoo Byun
Yun Haeng Lee
Song Seok Shin
Joon Tae Park
author_sort Ji Ho Park
collection DOAJ
description <b>Background:</b> Reactive oxygen species (ROS) generated by mitochondrial dysfunction damage cellular organelles and contribute to skin aging. Therefore, strategies to reduce mitochondrial ROS production are considered important for alleviating skin aging, but no effective methods have been identified. <b>Methods:</b> In this study, we evaluated substances utilized as cosmetic ingredients and discovered <i>Camellia sinensis</i> (<i>C. sinensis</i>) as a substance that reduces mitochondrial ROS levels. <b>Results:</b> <i>C. sinensis</i> extracts were found to act as senolytics that selectively kill senescent fibroblasts containing dysfunctional mitochondria. In addition, <i>C. sinensis</i> extracts facilitated efficient electron transport in the mitochondrial electron transport chain (ETC) by increasing the efficiency of oxidative phosphorylation (OXPHOS), thereby reducing mitochondrial ROS production, a byproduct of the inefficient ETC. This novel mechanism of <i>C. sinensis</i> extracts led to the restoration of skin aging and the skin barrier. Furthermore, epigallocatechin gallate (EGCG) was identified as an active ingredient that plays a key role in <i>C. sinensis</i> extract-mediated skin aging recovery. Indeed, similar to <i>C. sinensis</i> extracts, EGCG reduced ROS and improved skin aging in an artificial skin model. <b>Conclusions:</b> Our data uncovered a novel mechanism by which <i>C. sinensis</i> extract reverses skin aging by reducing mitochondrial ROS production via selective senescent cell death/increased OXPHOS efficiency. Our results suggest that <i>C. sinensis</i> extract or EGCG may be used as a therapeutic agent to reverse skin aging in clinical and cosmetic applications.
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spelling doaj-art-ccaf7d3114b84d25a327de9e7066bdca2025-08-20T01:56:35ZengMDPI AGPharmaceuticals1424-82472025-04-0118561210.3390/ph18050612Epigallocatechin Gallate in <i>Camellia sinensis</i> Ameliorates Skin Aging by Reducing Mitochondrial ROS ProductionJi Ho Park0Eun Young Jeong1Ye Hyang Kim2So Yoon Cha3Ha Yeon Kim4Yeon Kyung Nam5Jin Seong Park6So Yeon Kim7Yoo Jin Lee8Jee Hee Yoon9Byeonghyeon So10Duyeol Kim11Minseon Kim12Youngjoo Byun13Yun Haeng Lee14Song Seok Shin15Joon Tae Park16Division of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University, Incheon 22012, Republic of KoreaHyundai Bioland Co., Ltd., 22, Osongsaengmyeong 2-ro, Osong-eup, Heungdeok-gu, Cheongju-si 28162, Republic of KoreaHyundai Bioland Co., Ltd., 22, Osongsaengmyeong 2-ro, Osong-eup, Heungdeok-gu, Cheongju-si 28162, Republic of KoreaHyundai Bioland Co., Ltd., 22, Osongsaengmyeong 2-ro, Osong-eup, Heungdeok-gu, Cheongju-si 28162, Republic of KoreaHyundai Bioland Co., Ltd., 22, Osongsaengmyeong 2-ro, Osong-eup, Heungdeok-gu, Cheongju-si 28162, Republic of KoreaHyundai Bioland Co., Ltd., 22, Osongsaengmyeong 2-ro, Osong-eup, Heungdeok-gu, Cheongju-si 28162, Republic of KoreaHyundai Bioland Co., Ltd., 22, Osongsaengmyeong 2-ro, Osong-eup, Heungdeok-gu, Cheongju-si 28162, Republic of KoreaHyundai Bioland Co., Ltd., 22, Osongsaengmyeong 2-ro, Osong-eup, Heungdeok-gu, Cheongju-si 28162, Republic of KoreaDivision of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University, Incheon 22012, Republic of KoreaDivision of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University, Incheon 22012, Republic of KoreaDivision of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University, Incheon 22012, Republic of KoreaDivision of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University, Incheon 22012, Republic of KoreaDivision of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University, Incheon 22012, Republic of KoreaCollege of Pharmacy, Korea University, Sejong 30019, Republic of KoreaDivision of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University, Incheon 22012, Republic of KoreaHyundai Bioland Co., Ltd., 22, Osongsaengmyeong 2-ro, Osong-eup, Heungdeok-gu, Cheongju-si 28162, Republic of KoreaDivision of Life Sciences, College of Life Sciences and Bioengineering, Incheon National University, Incheon 22012, Republic of Korea<b>Background:</b> Reactive oxygen species (ROS) generated by mitochondrial dysfunction damage cellular organelles and contribute to skin aging. Therefore, strategies to reduce mitochondrial ROS production are considered important for alleviating skin aging, but no effective methods have been identified. <b>Methods:</b> In this study, we evaluated substances utilized as cosmetic ingredients and discovered <i>Camellia sinensis</i> (<i>C. sinensis</i>) as a substance that reduces mitochondrial ROS levels. <b>Results:</b> <i>C. sinensis</i> extracts were found to act as senolytics that selectively kill senescent fibroblasts containing dysfunctional mitochondria. In addition, <i>C. sinensis</i> extracts facilitated efficient electron transport in the mitochondrial electron transport chain (ETC) by increasing the efficiency of oxidative phosphorylation (OXPHOS), thereby reducing mitochondrial ROS production, a byproduct of the inefficient ETC. This novel mechanism of <i>C. sinensis</i> extracts led to the restoration of skin aging and the skin barrier. Furthermore, epigallocatechin gallate (EGCG) was identified as an active ingredient that plays a key role in <i>C. sinensis</i> extract-mediated skin aging recovery. Indeed, similar to <i>C. sinensis</i> extracts, EGCG reduced ROS and improved skin aging in an artificial skin model. <b>Conclusions:</b> Our data uncovered a novel mechanism by which <i>C. sinensis</i> extract reverses skin aging by reducing mitochondrial ROS production via selective senescent cell death/increased OXPHOS efficiency. Our results suggest that <i>C. sinensis</i> extract or EGCG may be used as a therapeutic agent to reverse skin aging in clinical and cosmetic applications.https://www.mdpi.com/1424-8247/18/5/612<i>Camellia sinensis</i>reactive oxygen species (ROS)senescence rejuvenationskin aging recovery
spellingShingle Ji Ho Park
Eun Young Jeong
Ye Hyang Kim
So Yoon Cha
Ha Yeon Kim
Yeon Kyung Nam
Jin Seong Park
So Yeon Kim
Yoo Jin Lee
Jee Hee Yoon
Byeonghyeon So
Duyeol Kim
Minseon Kim
Youngjoo Byun
Yun Haeng Lee
Song Seok Shin
Joon Tae Park
Epigallocatechin Gallate in <i>Camellia sinensis</i> Ameliorates Skin Aging by Reducing Mitochondrial ROS Production
Pharmaceuticals
<i>Camellia sinensis</i>
reactive oxygen species (ROS)
senescence rejuvenation
skin aging recovery
title Epigallocatechin Gallate in <i>Camellia sinensis</i> Ameliorates Skin Aging by Reducing Mitochondrial ROS Production
title_full Epigallocatechin Gallate in <i>Camellia sinensis</i> Ameliorates Skin Aging by Reducing Mitochondrial ROS Production
title_fullStr Epigallocatechin Gallate in <i>Camellia sinensis</i> Ameliorates Skin Aging by Reducing Mitochondrial ROS Production
title_full_unstemmed Epigallocatechin Gallate in <i>Camellia sinensis</i> Ameliorates Skin Aging by Reducing Mitochondrial ROS Production
title_short Epigallocatechin Gallate in <i>Camellia sinensis</i> Ameliorates Skin Aging by Reducing Mitochondrial ROS Production
title_sort epigallocatechin gallate in i camellia sinensis i ameliorates skin aging by reducing mitochondrial ros production
topic <i>Camellia sinensis</i>
reactive oxygen species (ROS)
senescence rejuvenation
skin aging recovery
url https://www.mdpi.com/1424-8247/18/5/612
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