Genomic characteristics of ST6115 carbapenem-resistant Klebsiella pneumoniae coharboring blaNDM-1 and blaIMP-4
ObjectivesThe aim of this study is to characterize the genomic features of ST6115 carbapenem-resistant Klebsiella pneumoniae (CRKP) co-harboring blaNDM-1 and blaIMP-4.MethodsThe genome sequences of one ST6115 Klebsiella pneumoniae (KP) strain and 12 ST17 KP strain were obtained through whole genome...
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1545161/full |
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| author | Xiaofang Xie Xiaofang Xie Yaxuan Zhai Zhichen Zhu Feinan Qian Feinan Qian Jie Zhu Qizhao Gao Hong Du Hong Du |
| author_facet | Xiaofang Xie Xiaofang Xie Yaxuan Zhai Zhichen Zhu Feinan Qian Feinan Qian Jie Zhu Qizhao Gao Hong Du Hong Du |
| author_sort | Xiaofang Xie |
| collection | DOAJ |
| description | ObjectivesThe aim of this study is to characterize the genomic features of ST6115 carbapenem-resistant Klebsiella pneumoniae (CRKP) co-harboring blaNDM-1 and blaIMP-4.MethodsThe genome sequences of one ST6115 Klebsiella pneumoniae (KP) strain and 12 ST17 KP strain were obtained through whole genome sequencing (WGS). Subsequently, a phylogenetic analysis was employed to ascertain the clonal relationship of these strains. The antimicrobial susceptibility testing (AST) was evaluated through the application of the minimum inhibitory concentration (MIC) methodology by the broth microdilution method. Annotation and analysis of the genome enabled the identification of the plasmid structure and the comparative analysis of the genetic background. Finally, the conjugation transfer experiment was conducted to assess the transferability of the plasmid pHD8428-NDM-IMP.ResultsA comparable phylogenetic analysis revealed that ST6115 HD8428 and the majority of ST17 strains (9/12) were clustered on the same clade, which suggests a close relationship between two ST types. Additionally, HD8428 showed particularly close genetic similarity to HD11490, with a single-nucleotide polymorphism (SNP) difference of only 273. The analysis of the antibiotic resistance genes carried by the 13 strains revealed that all strains carry extended-spectrum β-lactamase (ESBL) genes. AST revealed that HD8428 exhibited resistance to a diverse range of antibiotics. The blaNDM-1 and blaIMP-4 genes were identified as being located on the plasmid pHD8428-NDM-IMP. Further analysis demonstrated that the blaNDM-1 gene was present on ΔTn125, while the blaIMP-4 gene was located on In1377-2. The results of the conjugation experiment indicated that plasmid pHD8428-NDM-IMP may pose a risk for the transmission of antibiotic resistance in healthcare settings.ConclusionWe report a clinical ST6115 strain HD8428 and identify the coexistence of blaNDM-1 and blaIMP-4 in the IncHI5 type plasmid pHD8428-NDM-IMP. HD8428 was resistant to a wide range of antibiotics and harbored the transmissible plasmid pHD8428-NDM-IMP, which made it a potential threat to public health. Our study indicates that the healthcare system and services should remain vigilant regarding the spread and prevalence of ST6115. |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj-art-cc95d3bf26ac4361a95e34e6ac29e57e2025-02-05T07:32:27ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-02-011610.3389/fmicb.2025.15451611545161Genomic characteristics of ST6115 carbapenem-resistant Klebsiella pneumoniae coharboring blaNDM-1 and blaIMP-4Xiaofang Xie0Xiaofang Xie1Yaxuan Zhai2Zhichen Zhu3Feinan Qian4Feinan Qian5Jie Zhu6Qizhao Gao7Hong Du8Hong Du9Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, ChinaMOE Key Laboratory of Geriatric Diseases and Immunology, The Second Affiliated Hospital of Soochow University, Suzhou, ChinaDepartment of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, ChinaDepartment of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, ChinaDepartment of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, ChinaDepartment of Clinical Laboratory, Zhenjiang First People’s Hospital, Zhenjiang, ChinaDepartment of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, ChinaDepartment of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, ChinaDepartment of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, ChinaMOE Key Laboratory of Geriatric Diseases and Immunology, The Second Affiliated Hospital of Soochow University, Suzhou, ChinaObjectivesThe aim of this study is to characterize the genomic features of ST6115 carbapenem-resistant Klebsiella pneumoniae (CRKP) co-harboring blaNDM-1 and blaIMP-4.MethodsThe genome sequences of one ST6115 Klebsiella pneumoniae (KP) strain and 12 ST17 KP strain were obtained through whole genome sequencing (WGS). Subsequently, a phylogenetic analysis was employed to ascertain the clonal relationship of these strains. The antimicrobial susceptibility testing (AST) was evaluated through the application of the minimum inhibitory concentration (MIC) methodology by the broth microdilution method. Annotation and analysis of the genome enabled the identification of the plasmid structure and the comparative analysis of the genetic background. Finally, the conjugation transfer experiment was conducted to assess the transferability of the plasmid pHD8428-NDM-IMP.ResultsA comparable phylogenetic analysis revealed that ST6115 HD8428 and the majority of ST17 strains (9/12) were clustered on the same clade, which suggests a close relationship between two ST types. Additionally, HD8428 showed particularly close genetic similarity to HD11490, with a single-nucleotide polymorphism (SNP) difference of only 273. The analysis of the antibiotic resistance genes carried by the 13 strains revealed that all strains carry extended-spectrum β-lactamase (ESBL) genes. AST revealed that HD8428 exhibited resistance to a diverse range of antibiotics. The blaNDM-1 and blaIMP-4 genes were identified as being located on the plasmid pHD8428-NDM-IMP. Further analysis demonstrated that the blaNDM-1 gene was present on ΔTn125, while the blaIMP-4 gene was located on In1377-2. The results of the conjugation experiment indicated that plasmid pHD8428-NDM-IMP may pose a risk for the transmission of antibiotic resistance in healthcare settings.ConclusionWe report a clinical ST6115 strain HD8428 and identify the coexistence of blaNDM-1 and blaIMP-4 in the IncHI5 type plasmid pHD8428-NDM-IMP. HD8428 was resistant to a wide range of antibiotics and harbored the transmissible plasmid pHD8428-NDM-IMP, which made it a potential threat to public health. Our study indicates that the healthcare system and services should remain vigilant regarding the spread and prevalence of ST6115.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1545161/fullcarbapenem-resistant Klebsiella pneumoniaeST6115IncHI5 plasmidblaNDM-1blaIMP-4 |
| spellingShingle | Xiaofang Xie Xiaofang Xie Yaxuan Zhai Zhichen Zhu Feinan Qian Feinan Qian Jie Zhu Qizhao Gao Hong Du Hong Du Genomic characteristics of ST6115 carbapenem-resistant Klebsiella pneumoniae coharboring blaNDM-1 and blaIMP-4 Frontiers in Microbiology carbapenem-resistant Klebsiella pneumoniae ST6115 IncHI5 plasmid blaNDM-1 blaIMP-4 |
| title | Genomic characteristics of ST6115 carbapenem-resistant Klebsiella pneumoniae coharboring blaNDM-1 and blaIMP-4 |
| title_full | Genomic characteristics of ST6115 carbapenem-resistant Klebsiella pneumoniae coharboring blaNDM-1 and blaIMP-4 |
| title_fullStr | Genomic characteristics of ST6115 carbapenem-resistant Klebsiella pneumoniae coharboring blaNDM-1 and blaIMP-4 |
| title_full_unstemmed | Genomic characteristics of ST6115 carbapenem-resistant Klebsiella pneumoniae coharboring blaNDM-1 and blaIMP-4 |
| title_short | Genomic characteristics of ST6115 carbapenem-resistant Klebsiella pneumoniae coharboring blaNDM-1 and blaIMP-4 |
| title_sort | genomic characteristics of st6115 carbapenem resistant klebsiella pneumoniae coharboring blandm 1 and blaimp 4 |
| topic | carbapenem-resistant Klebsiella pneumoniae ST6115 IncHI5 plasmid blaNDM-1 blaIMP-4 |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1545161/full |
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