Adenosine deaminase promotes goose astrovirus genotype II replication in GEF cells
Goose astrovirus genotype II (GAstV-II), the causative agent of visceral and joint gout in goslings, has been widespread in China since 2016 and resulted in considerable economic losses to waterfowl industry. As an important enzyme involved in purine metabolism and uricogenesis, adenosine deaminase...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-07-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579125004183 |
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| author | Saimin Zhai Ruixue Li Keying Liu Huichao Gao Xia Yang Jun Zhao Xiaozhan Zhang Zeng Wang |
| author_facet | Saimin Zhai Ruixue Li Keying Liu Huichao Gao Xia Yang Jun Zhao Xiaozhan Zhang Zeng Wang |
| author_sort | Saimin Zhai |
| collection | DOAJ |
| description | Goose astrovirus genotype II (GAstV-II), the causative agent of visceral and joint gout in goslings, has been widespread in China since 2016 and resulted in considerable economic losses to waterfowl industry. As an important enzyme involved in purine metabolism and uricogenesis, adenosine deaminase (ADA) is reported to be upregulated via GAstV infection. However, knowledge about the regulatory role of ADA played during virus replication is still limited. In the present work, goose ADA (gADA) was firstly cloned from goose embryo fibroblasts (GEF) and phylogenetic analysis showed that it was highly homologous with duck ADA, sharing 96.6 % identity in nucleotide sequences. Moreover, GAstV-II infection promoted the production of gADA but did not change its cellular distribution pattern, which was evenly dispersed in the cytoplasm and nucleus. Further results demonstrated that ectopic expression of gADA significantly enhanced viral capsid protein expression and virus loads in GEF cells. Conversely, knockdown of gADA by siRNA played the opposite role in virus replication. Notably, gADA could directly interact with viral capsid protein, particularly with its C-terminal domain. Our data elucidated the regulatory role of gADA during GAstV-II infection, thereby laying a solid foundation to further explore its pathogenesis. |
| format | Article |
| id | doaj-art-cc9493ff2a1747beabbcb44879699d80 |
| institution | OA Journals |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-cc9493ff2a1747beabbcb44879699d802025-08-20T02:07:16ZengElsevierPoultry Science0032-57912025-07-01104710517610.1016/j.psj.2025.105176Adenosine deaminase promotes goose astrovirus genotype II replication in GEF cellsSaimin Zhai0Ruixue Li1Keying Liu2Huichao Gao3Xia Yang4Jun Zhao5Xiaozhan Zhang6Zeng Wang7College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China; Ministry of Education Key Laboratory for Animal Pathogens and Biosafety, Zhengzhou 450046, ChinaCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, ChinaCollege of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China; Ministry of Education Key Laboratory for Animal Pathogens and Biosafety, Zhengzhou 450046, China; Key Laboratory of Quality and Safety Control of Poultry Products, Ministry of Agriculture and Rural Affairs, Zhengzhou 450046, China; Corresponding author at: College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450046, China.Goose astrovirus genotype II (GAstV-II), the causative agent of visceral and joint gout in goslings, has been widespread in China since 2016 and resulted in considerable economic losses to waterfowl industry. As an important enzyme involved in purine metabolism and uricogenesis, adenosine deaminase (ADA) is reported to be upregulated via GAstV infection. However, knowledge about the regulatory role of ADA played during virus replication is still limited. In the present work, goose ADA (gADA) was firstly cloned from goose embryo fibroblasts (GEF) and phylogenetic analysis showed that it was highly homologous with duck ADA, sharing 96.6 % identity in nucleotide sequences. Moreover, GAstV-II infection promoted the production of gADA but did not change its cellular distribution pattern, which was evenly dispersed in the cytoplasm and nucleus. Further results demonstrated that ectopic expression of gADA significantly enhanced viral capsid protein expression and virus loads in GEF cells. Conversely, knockdown of gADA by siRNA played the opposite role in virus replication. Notably, gADA could directly interact with viral capsid protein, particularly with its C-terminal domain. Our data elucidated the regulatory role of gADA during GAstV-II infection, thereby laying a solid foundation to further explore its pathogenesis.http://www.sciencedirect.com/science/article/pii/S0032579125004183GAstV-IIADACap proteinPositive regulationInteraction |
| spellingShingle | Saimin Zhai Ruixue Li Keying Liu Huichao Gao Xia Yang Jun Zhao Xiaozhan Zhang Zeng Wang Adenosine deaminase promotes goose astrovirus genotype II replication in GEF cells Poultry Science GAstV-II ADA Cap protein Positive regulation Interaction |
| title | Adenosine deaminase promotes goose astrovirus genotype II replication in GEF cells |
| title_full | Adenosine deaminase promotes goose astrovirus genotype II replication in GEF cells |
| title_fullStr | Adenosine deaminase promotes goose astrovirus genotype II replication in GEF cells |
| title_full_unstemmed | Adenosine deaminase promotes goose astrovirus genotype II replication in GEF cells |
| title_short | Adenosine deaminase promotes goose astrovirus genotype II replication in GEF cells |
| title_sort | adenosine deaminase promotes goose astrovirus genotype ii replication in gef cells |
| topic | GAstV-II ADA Cap protein Positive regulation Interaction |
| url | http://www.sciencedirect.com/science/article/pii/S0032579125004183 |
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