Multi-omics revealed the promoting effect of dietary inulin supplementation on hepatic gluconeogenesis in goats
IntroductionGluconeogenesis is the primary pathway for ruminants to obtain energy. Enhancement of gluconeogenesis can significantly improve the growth performance of ruminants. Inulin, a prebiotic, has capabilities such as fostering a healthier gut microbiota and modulating metabolism. However, the...
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Frontiers Media S.A.
2025-07-01
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| author | Dan Zhu Dan Zhu Yun Hu Yun Hu Yong Liu Chuanshe Zhou Zhiliang Tan Jinhe Kang Zheng Wang |
| author_facet | Dan Zhu Dan Zhu Yun Hu Yun Hu Yong Liu Chuanshe Zhou Zhiliang Tan Jinhe Kang Zheng Wang |
| author_sort | Dan Zhu |
| collection | DOAJ |
| description | IntroductionGluconeogenesis is the primary pathway for ruminants to obtain energy. Enhancement of gluconeogenesis can significantly improve the growth performance of ruminants. Inulin, a prebiotic, has capabilities such as fostering a healthier gut microbiota and modulating metabolism. However, the application of inulin in ruminant feed is still very limited.MethodsEighteen healthy Xiangdong black goats (body weight 9.0 ± 0.19 kg) were randomly divided into two groups: the control group and the inulin group, with nine goats in each group. The inulin group used 18.9% inulin instead of normal corn. The total experimental period was 28 days, with 7 days for adaptation before the formal experiment.ResultsInulin supplementation significantly increased (P < 0.05) phosphoenolpyruvate carboxyl kinase (PEPCK) and glucose-6-phosphatase (G6Pase), as well as the expression of forkhead box protein O1 (FoxO1) in goat livers. At the same time, the serum insulin levels were significantly reduced (P < 0.05). Analysis of rumen microbes and rumen VFA levels revealed that the abundance levels of short-chain fatty acid-producing bacteria (Lachnospiracea, Blautia, Prevotella-1, and Pseudobutyrivibrio) and propionic acid concentration were significantly higher (P < 0.05) in the inulin group. Liver metabolites were analyzed via LC-MS, and increased levels of metabolites associated with the tricarboxylic acid (TCA) cycle and amino acid metabolism were observed following inulin administration.DiscussionInulin promotes the process of gluconeogenesis in goat liver by regulating the two key pathways of rumen microorganisms and liver metabolites to increase gluconeogenesis substrates. |
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| language | English |
| publishDate | 2025-07-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Animal Science |
| spelling | doaj-art-cc9351d3a80a4e0b911bdef9179ca68e2025-08-20T03:11:39ZengFrontiers Media S.A.Frontiers in Animal Science2673-62252025-07-01610.3389/fanim.2025.16148691614869Multi-omics revealed the promoting effect of dietary inulin supplementation on hepatic gluconeogenesis in goatsDan Zhu0Dan Zhu1Yun Hu2Yun Hu3Yong Liu4Chuanshe Zhou5Zhiliang Tan6Jinhe Kang7Zheng Wang8College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hunan, ChinaNational Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan, ChinaCollege of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hunan, ChinaNational Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan, ChinaNational Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan, ChinaNational Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan, ChinaNational Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan, ChinaNational Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, Hunan, ChinaCollege of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, Hunan, ChinaIntroductionGluconeogenesis is the primary pathway for ruminants to obtain energy. Enhancement of gluconeogenesis can significantly improve the growth performance of ruminants. Inulin, a prebiotic, has capabilities such as fostering a healthier gut microbiota and modulating metabolism. However, the application of inulin in ruminant feed is still very limited.MethodsEighteen healthy Xiangdong black goats (body weight 9.0 ± 0.19 kg) were randomly divided into two groups: the control group and the inulin group, with nine goats in each group. The inulin group used 18.9% inulin instead of normal corn. The total experimental period was 28 days, with 7 days for adaptation before the formal experiment.ResultsInulin supplementation significantly increased (P < 0.05) phosphoenolpyruvate carboxyl kinase (PEPCK) and glucose-6-phosphatase (G6Pase), as well as the expression of forkhead box protein O1 (FoxO1) in goat livers. At the same time, the serum insulin levels were significantly reduced (P < 0.05). Analysis of rumen microbes and rumen VFA levels revealed that the abundance levels of short-chain fatty acid-producing bacteria (Lachnospiracea, Blautia, Prevotella-1, and Pseudobutyrivibrio) and propionic acid concentration were significantly higher (P < 0.05) in the inulin group. Liver metabolites were analyzed via LC-MS, and increased levels of metabolites associated with the tricarboxylic acid (TCA) cycle and amino acid metabolism were observed following inulin administration.DiscussionInulin promotes the process of gluconeogenesis in goat liver by regulating the two key pathways of rumen microorganisms and liver metabolites to increase gluconeogenesis substrates.https://www.frontiersin.org/articles/10.3389/fanim.2025.1614869/fullinulingluconeogenesisruminal microorganismvolatile fatty acidmetabolite |
| spellingShingle | Dan Zhu Dan Zhu Yun Hu Yun Hu Yong Liu Chuanshe Zhou Zhiliang Tan Jinhe Kang Zheng Wang Multi-omics revealed the promoting effect of dietary inulin supplementation on hepatic gluconeogenesis in goats Frontiers in Animal Science inulin gluconeogenesis ruminal microorganism volatile fatty acid metabolite |
| title | Multi-omics revealed the promoting effect of dietary inulin supplementation on hepatic gluconeogenesis in goats |
| title_full | Multi-omics revealed the promoting effect of dietary inulin supplementation on hepatic gluconeogenesis in goats |
| title_fullStr | Multi-omics revealed the promoting effect of dietary inulin supplementation on hepatic gluconeogenesis in goats |
| title_full_unstemmed | Multi-omics revealed the promoting effect of dietary inulin supplementation on hepatic gluconeogenesis in goats |
| title_short | Multi-omics revealed the promoting effect of dietary inulin supplementation on hepatic gluconeogenesis in goats |
| title_sort | multi omics revealed the promoting effect of dietary inulin supplementation on hepatic gluconeogenesis in goats |
| topic | inulin gluconeogenesis ruminal microorganism volatile fatty acid metabolite |
| url | https://www.frontiersin.org/articles/10.3389/fanim.2025.1614869/full |
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