RP-HPLC Method for Simultaneous Estimation of Cefepime Hydrochloride and Tazobactam Sodium in Bulk and Pharmaceuticals
A precise, accurate, sensitive and robust RP-HPLC method was developed for cefepime hydrochloride and tazobactam sodium in bulk and pharmaceutical formulation. Chromatographic separation was achieved on PrincetonSPHER-100 C-18 column (250 mm × 4.6 mm i.d., 5 µm) at ambient temperature. A binary mobi...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2013/208057 |
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| author | Sagar R. Tamboli Dipak D. Patil |
| author_facet | Sagar R. Tamboli Dipak D. Patil |
| author_sort | Sagar R. Tamboli |
| collection | DOAJ |
| description | A precise, accurate, sensitive and robust RP-HPLC method was developed for cefepime hydrochloride and tazobactam sodium in bulk and pharmaceutical formulation. Chromatographic separation was achieved on PrincetonSPHER-100 C-18 column (250 mm × 4.6 mm i.d., 5 µm) at ambient temperature. A binary mobile phase consisting of 25 mM potassium dihydrogen phosphate buffer, pH 6.2 and acetonitrile (94 : 6, v/v) was delivered through a column at a flow rate of 1 mL/min. Measurement was performed at a wavelength 210 nm. The method was linear over the concentration range of 4–24 µg/mL () for cefepime and 0.5–3.0 µg/mL () for tazobactam. The percentage content found for cefepime was 101.12 ± 0.49 and for tazobactam was 101.33 ± 1.17 in the pharmaceutical formulation. The method was validated for linearity, precision, accuracy, sensitivity and robustness as per ICH Q2 (R1) guideline. |
| format | Article |
| id | doaj-art-cc863dacb54f4ce5be616cc9288abc82 |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-cc863dacb54f4ce5be616cc9288abc822025-02-03T01:09:11ZengWileyJournal of Chemistry2090-90632090-90712013-01-01201310.1155/2013/208057208057RP-HPLC Method for Simultaneous Estimation of Cefepime Hydrochloride and Tazobactam Sodium in Bulk and PharmaceuticalsSagar R. Tamboli0Dipak D. Patil1Department of Quality Assurance, H. R. Patel Institute of Pharmaceutical Education and Research, Karvand Naka, Shirpur 425405, IndiaDepartment of Quality Assurance, H. R. Patel Institute of Pharmaceutical Education and Research, Karvand Naka, Shirpur 425405, IndiaA precise, accurate, sensitive and robust RP-HPLC method was developed for cefepime hydrochloride and tazobactam sodium in bulk and pharmaceutical formulation. Chromatographic separation was achieved on PrincetonSPHER-100 C-18 column (250 mm × 4.6 mm i.d., 5 µm) at ambient temperature. A binary mobile phase consisting of 25 mM potassium dihydrogen phosphate buffer, pH 6.2 and acetonitrile (94 : 6, v/v) was delivered through a column at a flow rate of 1 mL/min. Measurement was performed at a wavelength 210 nm. The method was linear over the concentration range of 4–24 µg/mL () for cefepime and 0.5–3.0 µg/mL () for tazobactam. The percentage content found for cefepime was 101.12 ± 0.49 and for tazobactam was 101.33 ± 1.17 in the pharmaceutical formulation. The method was validated for linearity, precision, accuracy, sensitivity and robustness as per ICH Q2 (R1) guideline.http://dx.doi.org/10.1155/2013/208057 |
| spellingShingle | Sagar R. Tamboli Dipak D. Patil RP-HPLC Method for Simultaneous Estimation of Cefepime Hydrochloride and Tazobactam Sodium in Bulk and Pharmaceuticals Journal of Chemistry |
| title | RP-HPLC Method for Simultaneous Estimation of Cefepime Hydrochloride and Tazobactam Sodium in Bulk and Pharmaceuticals |
| title_full | RP-HPLC Method for Simultaneous Estimation of Cefepime Hydrochloride and Tazobactam Sodium in Bulk and Pharmaceuticals |
| title_fullStr | RP-HPLC Method for Simultaneous Estimation of Cefepime Hydrochloride and Tazobactam Sodium in Bulk and Pharmaceuticals |
| title_full_unstemmed | RP-HPLC Method for Simultaneous Estimation of Cefepime Hydrochloride and Tazobactam Sodium in Bulk and Pharmaceuticals |
| title_short | RP-HPLC Method for Simultaneous Estimation of Cefepime Hydrochloride and Tazobactam Sodium in Bulk and Pharmaceuticals |
| title_sort | rp hplc method for simultaneous estimation of cefepime hydrochloride and tazobactam sodium in bulk and pharmaceuticals |
| url | http://dx.doi.org/10.1155/2013/208057 |
| work_keys_str_mv | AT sagarrtamboli rphplcmethodforsimultaneousestimationofcefepimehydrochlorideandtazobactamsodiuminbulkandpharmaceuticals AT dipakdpatil rphplcmethodforsimultaneousestimationofcefepimehydrochlorideandtazobactamsodiuminbulkandpharmaceuticals |