Leguminous Lectins as Tools for Studying the Role of Sugar Residues in Leukocyte Recruitment
The natural physiological ligands for selectins are oligosaccharides found in glycoprotein or glycolipid molecules in cell membranes. In order to study the role of sugar residues in the in vivo lectin anti-inflammatory effect, we tested three leguminous lectins with different carbohydrate binding af...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
1999-01-01
|
| Series: | Mediators of Inflammation |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09629359990603 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832562769578164224 |
|---|---|
| author | N. M. N. Alencar E. H. Teixeira A. M. S. Assreuy B. S. Cavada C. A. Flores R. A. Ribeiro |
| author_facet | N. M. N. Alencar E. H. Teixeira A. M. S. Assreuy B. S. Cavada C. A. Flores R. A. Ribeiro |
| author_sort | N. M. N. Alencar |
| collection | DOAJ |
| description | The natural physiological ligands for selectins are oligosaccharides found in glycoprotein or glycolipid molecules in cell membranes. In order to study the role of sugar residues in the in vivo lectin anti-inflammatory effect, we tested three leguminous lectins with different carbohydrate binding affinities in the peritonitis and paw oedema models induced by carrageenin in rats. L. sericeus lectin was more anti-inflammatory than D. virgata lectin, the effects being reversed by their specific binding sugars (N-acetylglucosamine and α-methylmannoside, respectively). However, V. macrocarpa, a galactose-specific lectin, was not anti-inflammatory. The proposed anti-inflammatory activity of lectins could be due to a blockage of neutrophil-selectin carbohydrate ligands. Thus, according to the present data, we suggest an important role for N-acetylglucosamine residue as the major ligand for selectins on rat neutrophil membranes. |
| format | Article |
| id | doaj-art-cc85f60edd794896bdcad79a0e5a08bf |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1999-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-cc85f60edd794896bdcad79a0e5a08bf2025-02-03T01:21:54ZengWileyMediators of Inflammation0962-93511466-18611999-01-018210711310.1080/09629359990603Leguminous Lectins as Tools for Studying the Role of Sugar Residues in Leukocyte RecruitmentN. M. N. Alencar0E. H. Teixeira1A. M. S. Assreuy2B. S. Cavada3C. A. Flores4R. A. Ribeiro5Department of Clinical and Toxicological Analysis, Federal University of Ceara, PO Box 3157, Rua Coronel Nunes de Melo 1127, Fortaleza, CE 60430.270, BrazilDepartment of Biochemistry and Molecular Biology, Federal University of Ceara, PO Box 3157, Rua Coronel Nunes de Melo 1127, Fortaleza, CE 60430.270, BrazilDepartment of Physiology and Pharmacology, Federal University of Ceara, PO Box 3157, Rua Coronel Nunes de Melo 1127, Fortaleza, CE 60430.270, BrazilDepartment of Biochemistry and Molecular Biology, Federal University of Ceara, PO Box 3157, Rua Coronel Nunes de Melo 1127, Fortaleza, CE 60430.270, BrazilDepartment of Physiology and Pharmacology, Federal University of Ceara, PO Box 3157, Rua Coronel Nunes de Melo 1127, Fortaleza, CE 60430.270, BrazilDepartment of Physiology and Pharmacology, Federal University of Ceara, PO Box 3157, Rua Coronel Nunes de Melo 1127, Fortaleza, CE 60430.270, BrazilThe natural physiological ligands for selectins are oligosaccharides found in glycoprotein or glycolipid molecules in cell membranes. In order to study the role of sugar residues in the in vivo lectin anti-inflammatory effect, we tested three leguminous lectins with different carbohydrate binding affinities in the peritonitis and paw oedema models induced by carrageenin in rats. L. sericeus lectin was more anti-inflammatory than D. virgata lectin, the effects being reversed by their specific binding sugars (N-acetylglucosamine and α-methylmannoside, respectively). However, V. macrocarpa, a galactose-specific lectin, was not anti-inflammatory. The proposed anti-inflammatory activity of lectins could be due to a blockage of neutrophil-selectin carbohydrate ligands. Thus, according to the present data, we suggest an important role for N-acetylglucosamine residue as the major ligand for selectins on rat neutrophil membranes.http://dx.doi.org/10.1080/09629359990603Anti-inflammatoryLeguminous lectinsNeutrophil migrationRat paw oedemaSugar residues. |
| spellingShingle | N. M. N. Alencar E. H. Teixeira A. M. S. Assreuy B. S. Cavada C. A. Flores R. A. Ribeiro Leguminous Lectins as Tools for Studying the Role of Sugar Residues in Leukocyte Recruitment Mediators of Inflammation Anti-inflammatory Leguminous lectins Neutrophil migration Rat paw oedema Sugar residues. |
| title | Leguminous Lectins as Tools for Studying the Role of Sugar Residues in Leukocyte Recruitment |
| title_full | Leguminous Lectins as Tools for Studying the Role of Sugar Residues in Leukocyte Recruitment |
| title_fullStr | Leguminous Lectins as Tools for Studying the Role of Sugar Residues in Leukocyte Recruitment |
| title_full_unstemmed | Leguminous Lectins as Tools for Studying the Role of Sugar Residues in Leukocyte Recruitment |
| title_short | Leguminous Lectins as Tools for Studying the Role of Sugar Residues in Leukocyte Recruitment |
| title_sort | leguminous lectins as tools for studying the role of sugar residues in leukocyte recruitment |
| topic | Anti-inflammatory Leguminous lectins Neutrophil migration Rat paw oedema Sugar residues. |
| url | http://dx.doi.org/10.1080/09629359990603 |
| work_keys_str_mv | AT nmnalencar leguminouslectinsastoolsforstudyingtheroleofsugarresiduesinleukocyterecruitment AT ehteixeira leguminouslectinsastoolsforstudyingtheroleofsugarresiduesinleukocyterecruitment AT amsassreuy leguminouslectinsastoolsforstudyingtheroleofsugarresiduesinleukocyterecruitment AT bscavada leguminouslectinsastoolsforstudyingtheroleofsugarresiduesinleukocyterecruitment AT caflores leguminouslectinsastoolsforstudyingtheroleofsugarresiduesinleukocyterecruitment AT raribeiro leguminouslectinsastoolsforstudyingtheroleofsugarresiduesinleukocyterecruitment |