Alterations in the “Gut–Liver Axis” on Rats with Immunological Hepatic Fibrosis
There remains a lack of standard models that have all the characteristics of human diseases. Especially in immunological hepatic fibrosis, the bovine serum albumin (BSA)-induced liver fibrosis models have the same developmental mechanisms as human liver fibrosis models, but have received little atte...
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| Format: | Article |
| Language: | English |
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Wiley
2023-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2023/5577850 |
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| author | Zhaoyao Qi Xinxin Qi Yuanhui Xu Hongguang Sun Dengfeng Li Jincun Liu Meili Cong Tao Liu |
| author_facet | Zhaoyao Qi Xinxin Qi Yuanhui Xu Hongguang Sun Dengfeng Li Jincun Liu Meili Cong Tao Liu |
| author_sort | Zhaoyao Qi |
| collection | DOAJ |
| description | There remains a lack of standard models that have all the characteristics of human diseases. Especially in immunological hepatic fibrosis, the bovine serum albumin (BSA)-induced liver fibrosis models have the same developmental mechanisms as human liver fibrosis models, but have received little attention. We standardized a BSA-induced liver fibrosis model in rats and thoroughly assessed its pathological characteristics. We also used 16S sequencing to assess homeostasis of the intestinal microflora of rats with BSA-induced liver fibrosis and detected various differential metabolites in the serum of these rats using ultrahigh-performance liquid chromatography–tandem mass spectrometry (UHPLC-MS/MS). We observed stable and unambiguous histological changes in liver tissue morphology and remarkably high concentrations of inflammatory markers in the serum of BSA-induced liver fibrosis rats. In keeping with the fact that BSA induction can cause gut microbiota disorders in rats. UHPLC-MS/MS analysis of rat serum samples in positive-ion mode and negative-ion mode revealed 17 and 25 differential metabolites, respectively. Network analysis revealed that phenylalanine or tyrosine metabolites (e.g., PAGln) were the predominant metabolites in the sera of BSA-induced liver fibrosis rats. Taken together, our results suggest that disorders of amino acid metabolism caused by the gut microbiota may play an important role in the progression of immunological hepatic fibrosis. |
| format | Article |
| id | doaj-art-cc7c5ce374484b85be98821a0bb0a1ea |
| institution | DOAJ |
| issn | 2314-7156 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-cc7c5ce374484b85be98821a0bb0a1ea2025-08-20T03:04:42ZengWileyJournal of Immunology Research2314-71562023-01-01202310.1155/2023/5577850Alterations in the “Gut–Liver Axis” on Rats with Immunological Hepatic FibrosisZhaoyao Qi0Xinxin Qi1Yuanhui Xu2Hongguang Sun3Dengfeng Li4Jincun Liu5Meili Cong6Tao Liu7School of Public HealthSchool of Public HealthSchool of Public HealthSchool of Public HealthSchool of Public HealthSchool of Public HealthSchool of Public HealthSchool of Public HealthThere remains a lack of standard models that have all the characteristics of human diseases. Especially in immunological hepatic fibrosis, the bovine serum albumin (BSA)-induced liver fibrosis models have the same developmental mechanisms as human liver fibrosis models, but have received little attention. We standardized a BSA-induced liver fibrosis model in rats and thoroughly assessed its pathological characteristics. We also used 16S sequencing to assess homeostasis of the intestinal microflora of rats with BSA-induced liver fibrosis and detected various differential metabolites in the serum of these rats using ultrahigh-performance liquid chromatography–tandem mass spectrometry (UHPLC-MS/MS). We observed stable and unambiguous histological changes in liver tissue morphology and remarkably high concentrations of inflammatory markers in the serum of BSA-induced liver fibrosis rats. In keeping with the fact that BSA induction can cause gut microbiota disorders in rats. UHPLC-MS/MS analysis of rat serum samples in positive-ion mode and negative-ion mode revealed 17 and 25 differential metabolites, respectively. Network analysis revealed that phenylalanine or tyrosine metabolites (e.g., PAGln) were the predominant metabolites in the sera of BSA-induced liver fibrosis rats. Taken together, our results suggest that disorders of amino acid metabolism caused by the gut microbiota may play an important role in the progression of immunological hepatic fibrosis.http://dx.doi.org/10.1155/2023/5577850 |
| spellingShingle | Zhaoyao Qi Xinxin Qi Yuanhui Xu Hongguang Sun Dengfeng Li Jincun Liu Meili Cong Tao Liu Alterations in the “Gut–Liver Axis” on Rats with Immunological Hepatic Fibrosis Journal of Immunology Research |
| title | Alterations in the “Gut–Liver Axis” on Rats with Immunological Hepatic Fibrosis |
| title_full | Alterations in the “Gut–Liver Axis” on Rats with Immunological Hepatic Fibrosis |
| title_fullStr | Alterations in the “Gut–Liver Axis” on Rats with Immunological Hepatic Fibrosis |
| title_full_unstemmed | Alterations in the “Gut–Liver Axis” on Rats with Immunological Hepatic Fibrosis |
| title_short | Alterations in the “Gut–Liver Axis” on Rats with Immunological Hepatic Fibrosis |
| title_sort | alterations in the gut liver axis on rats with immunological hepatic fibrosis |
| url | http://dx.doi.org/10.1155/2023/5577850 |
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