Oxygen saturation imaging elucidates tumor heterogeneity in gastric cancer
Abstract Oxygen saturation imaging is a new technology that determines biological features from the perspective of oxygen concentration. Therefore, this exploratory study aimed to evaluate the biological implications of oxygen saturation imaging and further assess tumor heterogeneity in gastric canc...
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| Format: | Article |
| Language: | English |
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Wiley
2025-04-01
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| Series: | DEN Open |
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| Online Access: | https://doi.org/10.1002/deo2.70077 |
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| author | Tatsunori Minamide Nobuhisa Minakata Riu Yamashita Shingo Sakashita Yusuke Yoda Akihiro Ohashi Masato Aoshima Susumu Kobayashi Tomonori Yano |
| author_facet | Tatsunori Minamide Nobuhisa Minakata Riu Yamashita Shingo Sakashita Yusuke Yoda Akihiro Ohashi Masato Aoshima Susumu Kobayashi Tomonori Yano |
| author_sort | Tatsunori Minamide |
| collection | DOAJ |
| description | Abstract Oxygen saturation imaging is a new technology that determines biological features from the perspective of oxygen concentration. Therefore, this exploratory study aimed to evaluate the biological implications of oxygen saturation imaging and further assess tumor heterogeneity in gastric cancer. Biopsy samples were selectively obtained from treatment‐naïve patients with gastric cancer under real‐time oxygen saturation imaging. Tissue oxygen saturation level calculations, immunohistochemistry, and RNA sequencing were performed. The mean tissue oxygen saturation levels at the sampling sites were 32.2%, 70.8%, and 56.2% for hypoxic, hyperoxic, and non‐tumor areas, respectively, with significant differences between each pair. CD‐31 and glucose transporter 1 protein expression were significantly upregulated in hypoxic tumors. Comprehensive transcriptomic analysis revealed enriched biological processes related to the regulation of insulin‐like growth factor transport and uptake by insulin‐like growth factor‐binding proteins in hypoxic tumors and the type I interferon signaling pathway in hyperoxic tumors. Oxygen saturation imaging has the potential to clarify hypoxia‐induced heterogeneity in gastric cancer from both clinical and fundamental perspectives. |
| format | Article |
| id | doaj-art-cc7bc02fc21e471ab5b87930046ec9f5 |
| institution | DOAJ |
| issn | 2692-4609 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Wiley |
| record_format | Article |
| series | DEN Open |
| spelling | doaj-art-cc7bc02fc21e471ab5b87930046ec9f52025-08-20T03:13:51ZengWileyDEN Open2692-46092025-04-0151n/an/a10.1002/deo2.70077Oxygen saturation imaging elucidates tumor heterogeneity in gastric cancerTatsunori Minamide0Nobuhisa Minakata1Riu Yamashita2Shingo Sakashita3Yusuke Yoda4Akihiro Ohashi5Masato Aoshima6Susumu Kobayashi7Tomonori Yano8Department of Gastroenterology and EndoscopyNational Cancer Center Hospital EastChibaJapanDepartment of Gastroenterology and EndoscopyNational Cancer Center Hospital EastChibaJapanDivision of Translational Informatics, Exploratory Oncology Research & Clinical Trial CenterNational Cancer CenterChibaJapanDepartment of Pathology and Clinical LaboratoriesNational Cancer Center Hospital EastChibaJapanDepartment of Gastroenterology and EndoscopyNational Cancer Center Hospital EastChibaJapanDivision of Translational Genomics, Exploratory Oncology Research & Clinical Trial CenterNational Cancer CenterChibaJapanDivision of Translational Informatics, Exploratory Oncology Research & Clinical Trial CenterNational Cancer CenterChibaJapanDivision of Translational Genomics, Exploratory Oncology Research & Clinical Trial CenterNational Cancer CenterChibaJapanDepartment of Gastroenterology and EndoscopyNational Cancer Center Hospital EastChibaJapanAbstract Oxygen saturation imaging is a new technology that determines biological features from the perspective of oxygen concentration. Therefore, this exploratory study aimed to evaluate the biological implications of oxygen saturation imaging and further assess tumor heterogeneity in gastric cancer. Biopsy samples were selectively obtained from treatment‐naïve patients with gastric cancer under real‐time oxygen saturation imaging. Tissue oxygen saturation level calculations, immunohistochemistry, and RNA sequencing were performed. The mean tissue oxygen saturation levels at the sampling sites were 32.2%, 70.8%, and 56.2% for hypoxic, hyperoxic, and non‐tumor areas, respectively, with significant differences between each pair. CD‐31 and glucose transporter 1 protein expression were significantly upregulated in hypoxic tumors. Comprehensive transcriptomic analysis revealed enriched biological processes related to the regulation of insulin‐like growth factor transport and uptake by insulin‐like growth factor‐binding proteins in hypoxic tumors and the type I interferon signaling pathway in hyperoxic tumors. Oxygen saturation imaging has the potential to clarify hypoxia‐induced heterogeneity in gastric cancer from both clinical and fundamental perspectives.https://doi.org/10.1002/deo2.70077endoscopyheterogeneityoxygen saturationstomach neoplasmstumor hypoxia |
| spellingShingle | Tatsunori Minamide Nobuhisa Minakata Riu Yamashita Shingo Sakashita Yusuke Yoda Akihiro Ohashi Masato Aoshima Susumu Kobayashi Tomonori Yano Oxygen saturation imaging elucidates tumor heterogeneity in gastric cancer DEN Open endoscopy heterogeneity oxygen saturation stomach neoplasms tumor hypoxia |
| title | Oxygen saturation imaging elucidates tumor heterogeneity in gastric cancer |
| title_full | Oxygen saturation imaging elucidates tumor heterogeneity in gastric cancer |
| title_fullStr | Oxygen saturation imaging elucidates tumor heterogeneity in gastric cancer |
| title_full_unstemmed | Oxygen saturation imaging elucidates tumor heterogeneity in gastric cancer |
| title_short | Oxygen saturation imaging elucidates tumor heterogeneity in gastric cancer |
| title_sort | oxygen saturation imaging elucidates tumor heterogeneity in gastric cancer |
| topic | endoscopy heterogeneity oxygen saturation stomach neoplasms tumor hypoxia |
| url | https://doi.org/10.1002/deo2.70077 |
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