Development of Validated Spectrophotometric Methods for Estimation of Ethacridine Lactate in Pharmaceutical Formulations
Ethacridine lactate (EAL) is an antiseptic in solutions of 0.1%. It is also used as an agent for second trimester abortion. Two simple and sensitive spectrophotometric methods (Method A and Method B) were developed for the estimation of EAL in pharmaceutical formulations. Method A is based on the co...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | E-Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2011/274361 |
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| author | Aziz Unnisa N. Manasa P. Lakshmi Ravali |
| author_facet | Aziz Unnisa N. Manasa P. Lakshmi Ravali |
| author_sort | Aziz Unnisa |
| collection | DOAJ |
| description | Ethacridine lactate (EAL) is an antiseptic in solutions of 0.1%. It is also used as an agent for second trimester abortion. Two simple and sensitive spectrophotometric methods (Method A and Method B) were developed for the estimation of EAL in pharmaceutical formulations. Method A is based on the condensation of the primary aromatic amino group of ethacridine lactate with an aromatic aldehyde (p-Dimethylamino- cinnamaldehyde) to form a chromophore with absorption maximum of 600nm. Method B is based on redox reaction followed by complex formation of EAL with IO3-Metol reagent to form a stable chromogen, which can be estimated at 520 nm. Method A obeys Beer’s law in the concentration range of 2 to 12 μg/mL and method B in the range of 20 to 100 μg/mL. Interference studies were conducted to see the influence of excipients with the proposed methods. The common excipients usually present in dosage forms do not interfere in the proposed method A and method B. The optical characteristics, regression analysis data and precision of the methods were also calculated. The methods were validated for use in routine quality control of EAL in pharmaceutical formulations. |
| format | Article |
| id | doaj-art-cc6cef3c945b45998e41586bea11cbaf |
| institution | Kabale University |
| issn | 0973-4945 2090-9810 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | E-Journal of Chemistry |
| spelling | doaj-art-cc6cef3c945b45998e41586bea11cbaf2025-02-03T06:10:53ZengWileyE-Journal of Chemistry0973-49452090-98102011-01-01841815181910.1155/2011/274361Development of Validated Spectrophotometric Methods for Estimation of Ethacridine Lactate in Pharmaceutical FormulationsAziz Unnisa0N. Manasa1P. Lakshmi Ravali2K.V.S.R Siddhartha College of Pharmaceutical Sciences, Vijayawada, AP-520010, IndiaK.V.S.R Siddhartha College of Pharmaceutical Sciences, Vijayawada, AP-520010, IndiaK.V.S.R Siddhartha College of Pharmaceutical Sciences, Vijayawada, AP-520010, IndiaEthacridine lactate (EAL) is an antiseptic in solutions of 0.1%. It is also used as an agent for second trimester abortion. Two simple and sensitive spectrophotometric methods (Method A and Method B) were developed for the estimation of EAL in pharmaceutical formulations. Method A is based on the condensation of the primary aromatic amino group of ethacridine lactate with an aromatic aldehyde (p-Dimethylamino- cinnamaldehyde) to form a chromophore with absorption maximum of 600nm. Method B is based on redox reaction followed by complex formation of EAL with IO3-Metol reagent to form a stable chromogen, which can be estimated at 520 nm. Method A obeys Beer’s law in the concentration range of 2 to 12 μg/mL and method B in the range of 20 to 100 μg/mL. Interference studies were conducted to see the influence of excipients with the proposed methods. The common excipients usually present in dosage forms do not interfere in the proposed method A and method B. The optical characteristics, regression analysis data and precision of the methods were also calculated. The methods were validated for use in routine quality control of EAL in pharmaceutical formulations.http://dx.doi.org/10.1155/2011/274361 |
| spellingShingle | Aziz Unnisa N. Manasa P. Lakshmi Ravali Development of Validated Spectrophotometric Methods for Estimation of Ethacridine Lactate in Pharmaceutical Formulations E-Journal of Chemistry |
| title | Development of Validated Spectrophotometric Methods for Estimation of Ethacridine Lactate in Pharmaceutical Formulations |
| title_full | Development of Validated Spectrophotometric Methods for Estimation of Ethacridine Lactate in Pharmaceutical Formulations |
| title_fullStr | Development of Validated Spectrophotometric Methods for Estimation of Ethacridine Lactate in Pharmaceutical Formulations |
| title_full_unstemmed | Development of Validated Spectrophotometric Methods for Estimation of Ethacridine Lactate in Pharmaceutical Formulations |
| title_short | Development of Validated Spectrophotometric Methods for Estimation of Ethacridine Lactate in Pharmaceutical Formulations |
| title_sort | development of validated spectrophotometric methods for estimation of ethacridine lactate in pharmaceutical formulations |
| url | http://dx.doi.org/10.1155/2011/274361 |
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