Genetic Transformation of Common Beans (<i>Phaseolus vulgaris</i>): Achievements and Challenges
Genetic transformation is a valuable tool for the development of plant varieties with desirable traits that are present in the species germplasm with low genetic variability, i.e., resistance to pests and diseases and nutritional improvements. Although transgenic and edited crops have been successfu...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-11-01
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| Series: | Agriculture |
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| Online Access: | https://www.mdpi.com/2077-0472/14/11/2060 |
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| author | Matheus da Costa Moura Patricia Valle Pinheiro Rosana Pereira Vianello Natália Lima de Sousa Josias Correa de Faria Francisco José Lima Aragão |
| author_facet | Matheus da Costa Moura Patricia Valle Pinheiro Rosana Pereira Vianello Natália Lima de Sousa Josias Correa de Faria Francisco José Lima Aragão |
| author_sort | Matheus da Costa Moura |
| collection | DOAJ |
| description | Genetic transformation is a valuable tool for the development of plant varieties with desirable traits that are present in the species germplasm with low genetic variability, i.e., resistance to pests and diseases and nutritional improvements. Although transgenic and edited crops have been successfully obtained for many plant species, it remains difficult for common beans (<i>Phaseolus vulgaris</i>), due to their recalcitrance to in vitro regeneration. This review discusses various methods employed, such as <i>Agrobacterium</i>-mediated transformation, biolistic (particle bombardment), and hairy root systems, noting their respective efficiencies and limitations. While there has been progress, including the development of the first transgenic common bean cultivar approved for commercialization (Embrapa 5.1), the article emphasizes the need for improved protocols and techniques for more efficient genetic transformation. It also touches upon the potential of gene editing technologies like CRISPR/Cas9 in overcoming existing challenges and facilitating the development of resilient bean varieties. |
| format | Article |
| id | doaj-art-cc61396ea00c4bce94ca3a0f0b773c1a |
| institution | OA Journals |
| issn | 2077-0472 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agriculture |
| spelling | doaj-art-cc61396ea00c4bce94ca3a0f0b773c1a2025-08-20T01:53:48ZengMDPI AGAgriculture2077-04722024-11-011411206010.3390/agriculture14112060Genetic Transformation of Common Beans (<i>Phaseolus vulgaris</i>): Achievements and ChallengesMatheus da Costa Moura0Patricia Valle Pinheiro1Rosana Pereira Vianello2Natália Lima de Sousa3Josias Correa de Faria4Francisco José Lima Aragão5Department of Molecular Biology, University of Brasilia, Brasilia 70910-900, BrazilEmbrapa Arroz e Feijão, Santo Antônio de Goiás 75375-000, BrazilEmbrapa Arroz e Feijão, Santo Antônio de Goiás 75375-000, BrazilEmbrapa Recursos Genéticos e Biotecnologia, Brasilia 70770-917, BrazilEmbrapa Arroz e Feijão, Santo Antônio de Goiás 75375-000, BrazilEmbrapa Recursos Genéticos e Biotecnologia, Brasilia 70770-917, BrazilGenetic transformation is a valuable tool for the development of plant varieties with desirable traits that are present in the species germplasm with low genetic variability, i.e., resistance to pests and diseases and nutritional improvements. Although transgenic and edited crops have been successfully obtained for many plant species, it remains difficult for common beans (<i>Phaseolus vulgaris</i>), due to their recalcitrance to in vitro regeneration. This review discusses various methods employed, such as <i>Agrobacterium</i>-mediated transformation, biolistic (particle bombardment), and hairy root systems, noting their respective efficiencies and limitations. While there has been progress, including the development of the first transgenic common bean cultivar approved for commercialization (Embrapa 5.1), the article emphasizes the need for improved protocols and techniques for more efficient genetic transformation. It also touches upon the potential of gene editing technologies like CRISPR/Cas9 in overcoming existing challenges and facilitating the development of resilient bean varieties.https://www.mdpi.com/2077-0472/14/11/2060plant breedinggenetically modified organismtransgenicin vitro regenerationLeguminosae<i>Agrobacterium tumefaciens</i> |
| spellingShingle | Matheus da Costa Moura Patricia Valle Pinheiro Rosana Pereira Vianello Natália Lima de Sousa Josias Correa de Faria Francisco José Lima Aragão Genetic Transformation of Common Beans (<i>Phaseolus vulgaris</i>): Achievements and Challenges Agriculture plant breeding genetically modified organism transgenic in vitro regeneration Leguminosae <i>Agrobacterium tumefaciens</i> |
| title | Genetic Transformation of Common Beans (<i>Phaseolus vulgaris</i>): Achievements and Challenges |
| title_full | Genetic Transformation of Common Beans (<i>Phaseolus vulgaris</i>): Achievements and Challenges |
| title_fullStr | Genetic Transformation of Common Beans (<i>Phaseolus vulgaris</i>): Achievements and Challenges |
| title_full_unstemmed | Genetic Transformation of Common Beans (<i>Phaseolus vulgaris</i>): Achievements and Challenges |
| title_short | Genetic Transformation of Common Beans (<i>Phaseolus vulgaris</i>): Achievements and Challenges |
| title_sort | genetic transformation of common beans i phaseolus vulgaris i achievements and challenges |
| topic | plant breeding genetically modified organism transgenic in vitro regeneration Leguminosae <i>Agrobacterium tumefaciens</i> |
| url | https://www.mdpi.com/2077-0472/14/11/2060 |
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