An outer membrane vesicle specific lipoprotein promotes Porphyromonas gingivalis aggregation on red blood cells
Porphyromonas gingivalis uses a variety of mechanisms to actively interact with and promote the hydrolysis of red blood cells (RBCs) to obtain iron in the form of heme. In this study, we investigated the function of lipoprotein PG1881 which was previously shown to be up-regulated during subsurface g...
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| Language: | English |
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Elsevier
2024-01-01
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| Series: | Current Research in Microbial Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666517424000312 |
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| author | Christina M. Rothenberger Manda Yu Hey-Min Kim Yee-Wai Cheung Yi-Wei Chang Mary Ellen Davey |
| author_facet | Christina M. Rothenberger Manda Yu Hey-Min Kim Yee-Wai Cheung Yi-Wei Chang Mary Ellen Davey |
| author_sort | Christina M. Rothenberger |
| collection | DOAJ |
| description | Porphyromonas gingivalis uses a variety of mechanisms to actively interact with and promote the hydrolysis of red blood cells (RBCs) to obtain iron in the form of heme. In this study, we investigated the function of lipoprotein PG1881 which was previously shown to be up-regulated during subsurface growth and selectively enriched on outer membrane vesicles (OMVs). Our results show that wildtype strain W83 formed large aggregates encompassing RBCs whereas the PG1881 deletion mutant remained predominately as individual cells. Using a PG1881 antibody, immunofluorescence revealed that the wildtype strain's aggregation to RBCs involves an extracellular matrix enriched with PG1881. Our findings discover that RBCs elicit cell aggregation and matrix formation by P. gingivalis and that this process is promoted by an OMV-specific lipoprotein. We propose this strategy is advantageous for nutrient acquisition as well as dissemination from the oral cavity and survival of this periodontal pathogen. |
| format | Article |
| id | doaj-art-cc5ad8d4df7544f0aea856d75376e6c7 |
| institution | OA Journals |
| issn | 2666-5174 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Research in Microbial Sciences |
| spelling | doaj-art-cc5ad8d4df7544f0aea856d75376e6c72025-08-20T02:30:34ZengElsevierCurrent Research in Microbial Sciences2666-51742024-01-01710024910.1016/j.crmicr.2024.100249An outer membrane vesicle specific lipoprotein promotes Porphyromonas gingivalis aggregation on red blood cellsChristina M. Rothenberger0Manda Yu1Hey-Min Kim2Yee-Wai Cheung3Yi-Wei Chang4Mary Ellen Davey5Department of Microbiology, ADA Forsyth Institute, Cambridge, MA 02142, USA; Department of Oral Microbiology, University of Florida College of Dentistry, University of Florida, Gainesville, FL, USADepartment of Microbiology, ADA Forsyth Institute, Cambridge, MA 02142, USADepartment of Microbiology, ADA Forsyth Institute, Cambridge, MA 02142, USADepartment of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA; Institute of Plant and Microbial Biology, Academia Sinica, Taipei, TaiwanDepartment of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA; Institute of Structural Biology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USADepartment of Microbiology, ADA Forsyth Institute, Cambridge, MA 02142, USA; Corresponding author.Porphyromonas gingivalis uses a variety of mechanisms to actively interact with and promote the hydrolysis of red blood cells (RBCs) to obtain iron in the form of heme. In this study, we investigated the function of lipoprotein PG1881 which was previously shown to be up-regulated during subsurface growth and selectively enriched on outer membrane vesicles (OMVs). Our results show that wildtype strain W83 formed large aggregates encompassing RBCs whereas the PG1881 deletion mutant remained predominately as individual cells. Using a PG1881 antibody, immunofluorescence revealed that the wildtype strain's aggregation to RBCs involves an extracellular matrix enriched with PG1881. Our findings discover that RBCs elicit cell aggregation and matrix formation by P. gingivalis and that this process is promoted by an OMV-specific lipoprotein. We propose this strategy is advantageous for nutrient acquisition as well as dissemination from the oral cavity and survival of this periodontal pathogen.http://www.sciencedirect.com/science/article/pii/S2666517424000312OMVsFimbriaeCell aggregationLipoproteinRed blood cellP. gingivalis |
| spellingShingle | Christina M. Rothenberger Manda Yu Hey-Min Kim Yee-Wai Cheung Yi-Wei Chang Mary Ellen Davey An outer membrane vesicle specific lipoprotein promotes Porphyromonas gingivalis aggregation on red blood cells Current Research in Microbial Sciences OMVs Fimbriae Cell aggregation Lipoprotein Red blood cell P. gingivalis |
| title | An outer membrane vesicle specific lipoprotein promotes Porphyromonas gingivalis aggregation on red blood cells |
| title_full | An outer membrane vesicle specific lipoprotein promotes Porphyromonas gingivalis aggregation on red blood cells |
| title_fullStr | An outer membrane vesicle specific lipoprotein promotes Porphyromonas gingivalis aggregation on red blood cells |
| title_full_unstemmed | An outer membrane vesicle specific lipoprotein promotes Porphyromonas gingivalis aggregation on red blood cells |
| title_short | An outer membrane vesicle specific lipoprotein promotes Porphyromonas gingivalis aggregation on red blood cells |
| title_sort | outer membrane vesicle specific lipoprotein promotes porphyromonas gingivalis aggregation on red blood cells |
| topic | OMVs Fimbriae Cell aggregation Lipoprotein Red blood cell P. gingivalis |
| url | http://www.sciencedirect.com/science/article/pii/S2666517424000312 |
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