Impact of probiotics on sleep quality and mood states in patients with insomnia: a systematic review and meta-analysis

BackgroundProbiotics have garnered increasing attention for their potential role in managing insomnia. This meta-analysis evaluated the effects of probiotics on sleep quality and mood in patients with insomnia.MethodsEight public databases were searched to identify relevant randomized controlled tri...

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Main Authors: Yi Liu, Yunfeng Yu, Shenghua Lu, Kang Tan, Pengfei Jiang, Pei Liu, Qinghua Peng
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-07-01
Series:Frontiers in Microbiology
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Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2025.1596990/full
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author Yi Liu
Yunfeng Yu
Shenghua Lu
Kang Tan
Pengfei Jiang
Pei Liu
Qinghua Peng
Qinghua Peng
author_facet Yi Liu
Yunfeng Yu
Shenghua Lu
Kang Tan
Pengfei Jiang
Pei Liu
Qinghua Peng
Qinghua Peng
author_sort Yi Liu
collection DOAJ
description BackgroundProbiotics have garnered increasing attention for their potential role in managing insomnia. This meta-analysis evaluated the effects of probiotics on sleep quality and mood in patients with insomnia.MethodsEight public databases were searched to identify relevant randomized controlled trials (RCTs) published before December 2024. Data from included studies were extracted, and their risk of bias was assessed. Meta-analysis, sensitivity analysis, and publication bias assessment were conducted using Review Manager 5.3 software. The certainty of evidence was evaluated using Grading of Recommendations Assessment, Development and Evaluation (GRADE) system.ResultsSix studies, encompassing 424 patients, were included. Compared to control groups, the probiotic interventions were associated with a significant reduction in Pittsburgh Sleep Quality Index (PSQI) (mean difference [MD] −2.10, 95% confidence interval [CI] −3.86 to −0.34, p = 0.02, GRADE: moderate) and Hamilton Depression Scale (HAMD) (MD −7.72, 95% CI −14.55 to −0.89, p = 0.03, GRADE: very low) scores. However, no significant effects were observed on total sleep time (MD 43.70, 95% CI −18.07 to 105.46, p = 0.17, GRADE: very low), sleep efficiency (MD 0.41, 95% CI −1.67 to 2.48, p = 0.70, GRADE: moderate), or sleep latency (MD −4.74, 95% CI −9.42 to −0.05, p = 0.05, GRADE: moderate). Additionally, no significant differences in total adverse events were noted between probiotic and control groups. Funnel plots indicated no publication bias for PSQI, sleep efficiency, or sleep latency, whereas potential publication bias was detected for HAMD, total sleep time, and total adverse events.ConclusionProbiotic interventions improved sleep quality and reduced depressive symptoms in patients with insomnia without increasing the risk of adverse events. These findings highlight the potential of probiotics as complementary treatments for insomnia. However, due to the limited sample size, further high-quality clinical studies are warranted to confirm these findings.Systematic review registrationhttps://www.crd.york.ac.uk/PROSPERO/view/CRD420251077696, identifier CRD420251077696.
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spelling doaj-art-cc588367662c4d078b084cae55939f8a2025-08-20T03:13:10ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-07-011610.3389/fmicb.2025.15969901596990Impact of probiotics on sleep quality and mood states in patients with insomnia: a systematic review and meta-analysisYi Liu0Yunfeng Yu1Shenghua Lu2Kang Tan3Pengfei Jiang4Pei Liu5Qinghua Peng6Qinghua Peng7School of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, ChinaSchool of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, ChinaThe First Clinical College of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, ChinaSchool of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, ChinaQuzhou Hospital Ophthalmology Center, Zhejiang Medical and Health University, Quzhou, ChinaSchool of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, ChinaSchool of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, ChinaThe First Clinical College of Traditional Chinese Medicine, Hunan University of Chinese Medicine, Changsha, ChinaBackgroundProbiotics have garnered increasing attention for their potential role in managing insomnia. This meta-analysis evaluated the effects of probiotics on sleep quality and mood in patients with insomnia.MethodsEight public databases were searched to identify relevant randomized controlled trials (RCTs) published before December 2024. Data from included studies were extracted, and their risk of bias was assessed. Meta-analysis, sensitivity analysis, and publication bias assessment were conducted using Review Manager 5.3 software. The certainty of evidence was evaluated using Grading of Recommendations Assessment, Development and Evaluation (GRADE) system.ResultsSix studies, encompassing 424 patients, were included. Compared to control groups, the probiotic interventions were associated with a significant reduction in Pittsburgh Sleep Quality Index (PSQI) (mean difference [MD] −2.10, 95% confidence interval [CI] −3.86 to −0.34, p = 0.02, GRADE: moderate) and Hamilton Depression Scale (HAMD) (MD −7.72, 95% CI −14.55 to −0.89, p = 0.03, GRADE: very low) scores. However, no significant effects were observed on total sleep time (MD 43.70, 95% CI −18.07 to 105.46, p = 0.17, GRADE: very low), sleep efficiency (MD 0.41, 95% CI −1.67 to 2.48, p = 0.70, GRADE: moderate), or sleep latency (MD −4.74, 95% CI −9.42 to −0.05, p = 0.05, GRADE: moderate). Additionally, no significant differences in total adverse events were noted between probiotic and control groups. Funnel plots indicated no publication bias for PSQI, sleep efficiency, or sleep latency, whereas potential publication bias was detected for HAMD, total sleep time, and total adverse events.ConclusionProbiotic interventions improved sleep quality and reduced depressive symptoms in patients with insomnia without increasing the risk of adverse events. These findings highlight the potential of probiotics as complementary treatments for insomnia. However, due to the limited sample size, further high-quality clinical studies are warranted to confirm these findings.Systematic review registrationhttps://www.crd.york.ac.uk/PROSPERO/view/CRD420251077696, identifier CRD420251077696.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1596990/fullinsomniasleep disordersprobioticssleep qualitymood statessystematic review
spellingShingle Yi Liu
Yunfeng Yu
Shenghua Lu
Kang Tan
Pengfei Jiang
Pei Liu
Qinghua Peng
Qinghua Peng
Impact of probiotics on sleep quality and mood states in patients with insomnia: a systematic review and meta-analysis
Frontiers in Microbiology
insomnia
sleep disorders
probiotics
sleep quality
mood states
systematic review
title Impact of probiotics on sleep quality and mood states in patients with insomnia: a systematic review and meta-analysis
title_full Impact of probiotics on sleep quality and mood states in patients with insomnia: a systematic review and meta-analysis
title_fullStr Impact of probiotics on sleep quality and mood states in patients with insomnia: a systematic review and meta-analysis
title_full_unstemmed Impact of probiotics on sleep quality and mood states in patients with insomnia: a systematic review and meta-analysis
title_short Impact of probiotics on sleep quality and mood states in patients with insomnia: a systematic review and meta-analysis
title_sort impact of probiotics on sleep quality and mood states in patients with insomnia a systematic review and meta analysis
topic insomnia
sleep disorders
probiotics
sleep quality
mood states
systematic review
url https://www.frontiersin.org/articles/10.3389/fmicb.2025.1596990/full
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