Anti-CdtB and anti-vinculin antibodies to diagnose irritable bowel syndrome in inflammatory bowel disease patients
Abstract Background Despite adequate treatment, a subgroup of patients with inflammatory bowel disease (IBD), including Crohn`s disease and ulcerative colitis, have persistent gastrointestinal symptoms that are not always related to mucosal damage. Recently, two autoantibodies, anti-CdtB and anti-vi...
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2024-12-01
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| Online Access: | https://doi.org/10.1186/s12876-024-03509-z |
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| author | Luisa Leite Barros Gabriela Leite Walter Morales Gillian M. Barlow Matheus Freitas Cardoso de Azevedo Alexandre de Sousa Carlos Adérson Omar Mourão Cintra Damião Mark Pimentel Alberto Queiroz Farias |
| author_facet | Luisa Leite Barros Gabriela Leite Walter Morales Gillian M. Barlow Matheus Freitas Cardoso de Azevedo Alexandre de Sousa Carlos Adérson Omar Mourão Cintra Damião Mark Pimentel Alberto Queiroz Farias |
| author_sort | Luisa Leite Barros |
| collection | DOAJ |
| description | Abstract Background Despite adequate treatment, a subgroup of patients with inflammatory bowel disease (IBD), including Crohn`s disease and ulcerative colitis, have persistent gastrointestinal symptoms that are not always related to mucosal damage. Recently, two autoantibodies, anti-CdtB and anti-vinculin, were validated as post-infectious IBS (PI-IBS) markers, however there is limited evidence of its diagnostic role in IBD population. Methods Patients with more than 3 bowel movements/day and indication of colonoscopy were enrolled. Samples were collected at the time of colonoscopy for assessment of serum levels of anti-CdtB and anti-vinculin antibodies. Results A total of 160 subjects were included in 4 groups: active IBD (n = 44); quiescent IBD and chronic diarrhea IBD-IBS (n = 25); predominant-diarrhea IBS (n = 45) and controls (n = 46). The mean value of the optical density for anti-CdtB was 1.2 ± 0.65 in group 1, 1.27 ± 0.64 in group 2, 1.49 ± 0.47 in the group 3 and 1.6 ± 0.68 in group 4, p = 0.012. For anti-vinculin, optical densities were: 1.34 ± 0.78 in group 1, 1.46 ± 0.92 in group 2, 1.31 ± 0.79 in group 3 and 1.41 ± 0.86 for controls (p = 0.875). Using a cut-off of 1.56 for anti-CdtB, the positivity between groups was n = 10 (22.7%) in group 1, n = 9 (34.6%) in group 2, 19 (43.2%) in group 3, 21 (45.7%) in group 4 (p = 0.106). The positivity of anti-vinculin using a cut-off of 1.6 was n = 18 (40.9%) in group 1, n = 11 (42.3%), n = 15 (34.1%), n = 22 (47.8%) (p = 0.622). Conclusions Our findings show that anti-CdtB and anti-vinculin could not identify IBD-IBS patients or discriminate IBS-D from healthy controls. |
| format | Article |
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| spelling | doaj-art-cc4a523605824fefa93ca337d050bbea2025-08-20T02:31:00ZengBMCBMC Gastroenterology1471-230X2024-12-012411810.1186/s12876-024-03509-zAnti-CdtB and anti-vinculin antibodies to diagnose irritable bowel syndrome in inflammatory bowel disease patientsLuisa Leite Barros0Gabriela Leite1Walter Morales2Gillian M. Barlow3Matheus Freitas Cardoso de Azevedo4Alexandre de Sousa Carlos5Adérson Omar Mourão Cintra Damião6Mark Pimentel7Alberto Queiroz Farias8Department of Gastroenterology, University of Sao Paulo School of MedicineMedically Associated Science and Technology (MAST) Program, Cedars-Sinai Medical CenterMedically Associated Science and Technology (MAST) Program, Cedars-Sinai Medical CenterMedically Associated Science and Technology (MAST) Program, Cedars-Sinai Medical CenterDepartment of Gastroenterology, University of Sao Paulo School of MedicineDepartment of Gastroenterology, University of Sao Paulo School of MedicineDepartment of Gastroenterology, University of Sao Paulo School of MedicineMedically Associated Science and Technology (MAST) Program, Cedars-Sinai Medical CenterDepartment of Gastroenterology, University of Sao Paulo School of MedicineAbstract Background Despite adequate treatment, a subgroup of patients with inflammatory bowel disease (IBD), including Crohn`s disease and ulcerative colitis, have persistent gastrointestinal symptoms that are not always related to mucosal damage. Recently, two autoantibodies, anti-CdtB and anti-vinculin, were validated as post-infectious IBS (PI-IBS) markers, however there is limited evidence of its diagnostic role in IBD population. Methods Patients with more than 3 bowel movements/day and indication of colonoscopy were enrolled. Samples were collected at the time of colonoscopy for assessment of serum levels of anti-CdtB and anti-vinculin antibodies. Results A total of 160 subjects were included in 4 groups: active IBD (n = 44); quiescent IBD and chronic diarrhea IBD-IBS (n = 25); predominant-diarrhea IBS (n = 45) and controls (n = 46). The mean value of the optical density for anti-CdtB was 1.2 ± 0.65 in group 1, 1.27 ± 0.64 in group 2, 1.49 ± 0.47 in the group 3 and 1.6 ± 0.68 in group 4, p = 0.012. For anti-vinculin, optical densities were: 1.34 ± 0.78 in group 1, 1.46 ± 0.92 in group 2, 1.31 ± 0.79 in group 3 and 1.41 ± 0.86 for controls (p = 0.875). Using a cut-off of 1.56 for anti-CdtB, the positivity between groups was n = 10 (22.7%) in group 1, n = 9 (34.6%) in group 2, 19 (43.2%) in group 3, 21 (45.7%) in group 4 (p = 0.106). The positivity of anti-vinculin using a cut-off of 1.6 was n = 18 (40.9%) in group 1, n = 11 (42.3%), n = 15 (34.1%), n = 22 (47.8%) (p = 0.622). Conclusions Our findings show that anti-CdtB and anti-vinculin could not identify IBD-IBS patients or discriminate IBS-D from healthy controls.https://doi.org/10.1186/s12876-024-03509-zInflammatory bowel diseaseIrritable bowel syndromeAnti-CdtBAnti-vinculin |
| spellingShingle | Luisa Leite Barros Gabriela Leite Walter Morales Gillian M. Barlow Matheus Freitas Cardoso de Azevedo Alexandre de Sousa Carlos Adérson Omar Mourão Cintra Damião Mark Pimentel Alberto Queiroz Farias Anti-CdtB and anti-vinculin antibodies to diagnose irritable bowel syndrome in inflammatory bowel disease patients BMC Gastroenterology Inflammatory bowel disease Irritable bowel syndrome Anti-CdtB Anti-vinculin |
| title | Anti-CdtB and anti-vinculin antibodies to diagnose irritable bowel syndrome in inflammatory bowel disease patients |
| title_full | Anti-CdtB and anti-vinculin antibodies to diagnose irritable bowel syndrome in inflammatory bowel disease patients |
| title_fullStr | Anti-CdtB and anti-vinculin antibodies to diagnose irritable bowel syndrome in inflammatory bowel disease patients |
| title_full_unstemmed | Anti-CdtB and anti-vinculin antibodies to diagnose irritable bowel syndrome in inflammatory bowel disease patients |
| title_short | Anti-CdtB and anti-vinculin antibodies to diagnose irritable bowel syndrome in inflammatory bowel disease patients |
| title_sort | anti cdtb and anti vinculin antibodies to diagnose irritable bowel syndrome in inflammatory bowel disease patients |
| topic | Inflammatory bowel disease Irritable bowel syndrome Anti-CdtB Anti-vinculin |
| url | https://doi.org/10.1186/s12876-024-03509-z |
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