Phenotypic Variation in a Four-Generation Family with Aniridia Carrying a Novel PAX6 Mutation

Aniridia is a congenital disease that affects almost all eye structures and is primarily caused by loss-of-function mutations in the PAX6 gene. The degree of vision loss in aniridia varies and is dependent on the extent of foveal, iris, and optic nerve hypoplasia and the presence of glaucoma, catara...

Full description

Saved in:
Bibliographic Details
Main Authors: Grace M. Wang, Lev Prasov, Hayder Al-Hasani, Colin E. R. Marrs, Sahil Tolia, Laurel Wiinikka-Buesser, Julia E. Richards, Brenda L. Bohnsack
Format: Article
Language:English
Published: Wiley 2018-01-01
Series:Journal of Ophthalmology
Online Access:http://dx.doi.org/10.1155/2018/5978293
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850173056772734976
author Grace M. Wang
Lev Prasov
Hayder Al-Hasani
Colin E. R. Marrs
Sahil Tolia
Laurel Wiinikka-Buesser
Julia E. Richards
Brenda L. Bohnsack
author_facet Grace M. Wang
Lev Prasov
Hayder Al-Hasani
Colin E. R. Marrs
Sahil Tolia
Laurel Wiinikka-Buesser
Julia E. Richards
Brenda L. Bohnsack
author_sort Grace M. Wang
collection DOAJ
description Aniridia is a congenital disease that affects almost all eye structures and is primarily caused by loss-of-function mutations in the PAX6 gene. The degree of vision loss in aniridia varies and is dependent on the extent of foveal, iris, and optic nerve hypoplasia and the presence of glaucoma, cataracts, and corneal opacification. Here, we describe a 4-generation family in which 7 individuals across 2 generations carry a novel disease-causing frameshift mutation (NM_000280.4(PAX6):c.565TC>T) in PAX6. This mutation results in an early stop codon in exon 8, which is predicted to cause nonsense-mediated decay of the truncated mRNA and a functionally null PAX6 allele. Family members with aniridia showed differences in multiple eye phenotypes including iris and optic nerve hypoplasia, congenital and acquired corneal opacification, glaucoma, and strabismus. Visual acuity ranged from 20/100 to less than 20/800. Patients who required surgical intervention for glaucoma or corneal opacification had worse visual outcomes. Our results show that family members carrying a novel PAX6 frameshift mutation have variable expressivity, leading to different ocular comorbidities and visual outcomes.
format Article
id doaj-art-cc4155e57d2d4017bc5ca6e6f5f53c88
institution OA Journals
issn 2090-004X
2090-0058
language English
publishDate 2018-01-01
publisher Wiley
record_format Article
series Journal of Ophthalmology
spelling doaj-art-cc4155e57d2d4017bc5ca6e6f5f53c882025-08-20T02:19:57ZengWileyJournal of Ophthalmology2090-004X2090-00582018-01-01201810.1155/2018/59782935978293Phenotypic Variation in a Four-Generation Family with Aniridia Carrying a Novel PAX6 MutationGrace M. Wang0Lev Prasov1Hayder Al-Hasani2Colin E. R. Marrs3Sahil Tolia4Laurel Wiinikka-Buesser5Julia E. Richards6Brenda L. Bohnsack7Department of Ophthalmology and Visual Sciences, W.K. Kellogg Eye Center, The University of Michigan, Ann Arbor, MI 48105, USADepartment of Ophthalmology and Visual Sciences, W.K. Kellogg Eye Center, The University of Michigan, Ann Arbor, MI 48105, USADepartment of Ophthalmology and Visual Sciences, W.K. Kellogg Eye Center, The University of Michigan, Ann Arbor, MI 48105, USADepartment of Ophthalmology and Visual Sciences, W.K. Kellogg Eye Center, The University of Michigan, Ann Arbor, MI 48105, USADepartment of Ophthalmology and Visual Sciences, W.K. Kellogg Eye Center, The University of Michigan, Ann Arbor, MI 48105, USADepartment of Ophthalmology and Visual Sciences, W.K. Kellogg Eye Center, The University of Michigan, Ann Arbor, MI 48105, USADepartment of Ophthalmology and Visual Sciences, W.K. Kellogg Eye Center, The University of Michigan, Ann Arbor, MI 48105, USADepartment of Ophthalmology and Visual Sciences, W.K. Kellogg Eye Center, The University of Michigan, Ann Arbor, MI 48105, USAAniridia is a congenital disease that affects almost all eye structures and is primarily caused by loss-of-function mutations in the PAX6 gene. The degree of vision loss in aniridia varies and is dependent on the extent of foveal, iris, and optic nerve hypoplasia and the presence of glaucoma, cataracts, and corneal opacification. Here, we describe a 4-generation family in which 7 individuals across 2 generations carry a novel disease-causing frameshift mutation (NM_000280.4(PAX6):c.565TC>T) in PAX6. This mutation results in an early stop codon in exon 8, which is predicted to cause nonsense-mediated decay of the truncated mRNA and a functionally null PAX6 allele. Family members with aniridia showed differences in multiple eye phenotypes including iris and optic nerve hypoplasia, congenital and acquired corneal opacification, glaucoma, and strabismus. Visual acuity ranged from 20/100 to less than 20/800. Patients who required surgical intervention for glaucoma or corneal opacification had worse visual outcomes. Our results show that family members carrying a novel PAX6 frameshift mutation have variable expressivity, leading to different ocular comorbidities and visual outcomes.http://dx.doi.org/10.1155/2018/5978293
spellingShingle Grace M. Wang
Lev Prasov
Hayder Al-Hasani
Colin E. R. Marrs
Sahil Tolia
Laurel Wiinikka-Buesser
Julia E. Richards
Brenda L. Bohnsack
Phenotypic Variation in a Four-Generation Family with Aniridia Carrying a Novel PAX6 Mutation
Journal of Ophthalmology
title Phenotypic Variation in a Four-Generation Family with Aniridia Carrying a Novel PAX6 Mutation
title_full Phenotypic Variation in a Four-Generation Family with Aniridia Carrying a Novel PAX6 Mutation
title_fullStr Phenotypic Variation in a Four-Generation Family with Aniridia Carrying a Novel PAX6 Mutation
title_full_unstemmed Phenotypic Variation in a Four-Generation Family with Aniridia Carrying a Novel PAX6 Mutation
title_short Phenotypic Variation in a Four-Generation Family with Aniridia Carrying a Novel PAX6 Mutation
title_sort phenotypic variation in a four generation family with aniridia carrying a novel pax6 mutation
url http://dx.doi.org/10.1155/2018/5978293
work_keys_str_mv AT gracemwang phenotypicvariationinafourgenerationfamilywithaniridiacarryinganovelpax6mutation
AT levprasov phenotypicvariationinafourgenerationfamilywithaniridiacarryinganovelpax6mutation
AT hayderalhasani phenotypicvariationinafourgenerationfamilywithaniridiacarryinganovelpax6mutation
AT colinermarrs phenotypicvariationinafourgenerationfamilywithaniridiacarryinganovelpax6mutation
AT sahiltolia phenotypicvariationinafourgenerationfamilywithaniridiacarryinganovelpax6mutation
AT laurelwiinikkabuesser phenotypicvariationinafourgenerationfamilywithaniridiacarryinganovelpax6mutation
AT juliaerichards phenotypicvariationinafourgenerationfamilywithaniridiacarryinganovelpax6mutation
AT brendalbohnsack phenotypicvariationinafourgenerationfamilywithaniridiacarryinganovelpax6mutation