Molecular Mechanisms of Treadmill Therapy on Neuromuscular Atrophy Induced via Botulinum Toxin A

Botulinum toxin A (BoNT-A) is a bacterial zinc-dependent endopeptidase that acts specifically on neuromuscular junctions. BoNT-A blocks the release of acetylcholine, thereby decreasing the ability of a spastic muscle to generate forceful contraction, which results in a temporal local weakness and th...

Full description

Saved in:
Bibliographic Details
Main Authors: Sen-Wei Tsai, Hsiao-Ling Chen, Yi-Chun Chang, Chuan-Mu Chen
Format: Article
Language:English
Published: Wiley 2013-01-01
Series:Neural Plasticity
Online Access:http://dx.doi.org/10.1155/2013/593271
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850173053210722304
author Sen-Wei Tsai
Hsiao-Ling Chen
Yi-Chun Chang
Chuan-Mu Chen
author_facet Sen-Wei Tsai
Hsiao-Ling Chen
Yi-Chun Chang
Chuan-Mu Chen
author_sort Sen-Wei Tsai
collection DOAJ
description Botulinum toxin A (BoNT-A) is a bacterial zinc-dependent endopeptidase that acts specifically on neuromuscular junctions. BoNT-A blocks the release of acetylcholine, thereby decreasing the ability of a spastic muscle to generate forceful contraction, which results in a temporal local weakness and the atrophy of targeted muscles. BoNT-A-induced temporal muscle weakness has been used to manage skeletal muscle spasticity, such as poststroke spasticity, cerebral palsy, and cervical dystonia. However, the combined effect of treadmill exercise and BoNT-A treatment is not well understood. We previously demonstrated that for rats, following BoNT-A injection in the gastrocnemius muscle, treadmill running improved the recovery of the sciatic functional index (SFI), muscle contraction strength, and compound muscle action potential (CMAP) amplitude and area. Treadmill training had no influence on gastrocnemius mass that received BoNT-A injection, but it improved the maximal contraction force of the gastrocnemius, and upregulation of GAP-43, IGF-1, Myo-D, Myf-5, myogenin, and acetylcholine receptor (AChR) subunits α and β was found following treadmill training. Taken together, these results suggest that the upregulation of genes associated with neurite and AChR regeneration following treadmill training may contribute to enhanced gastrocnemius strength recovery following BoNT-A injection.
format Article
id doaj-art-cc31f8cb7bd74f179e533ba5bb1fe5cc
institution OA Journals
issn 2090-5904
1687-5443
language English
publishDate 2013-01-01
publisher Wiley
record_format Article
series Neural Plasticity
spelling doaj-art-cc31f8cb7bd74f179e533ba5bb1fe5cc2025-08-20T02:19:57ZengWileyNeural Plasticity2090-59041687-54432013-01-01201310.1155/2013/593271593271Molecular Mechanisms of Treadmill Therapy on Neuromuscular Atrophy Induced via Botulinum Toxin ASen-Wei Tsai0Hsiao-Ling Chen1Yi-Chun Chang2Chuan-Mu Chen3Department of Life Sciences, Agricultural Biotechnology Center, National Chung Hsing University, Taichung 402, TaiwanDepartment of Bioresources, Da-Yeh University, Changhua 515, TaiwanDepartment of Life Sciences, Agricultural Biotechnology Center, National Chung Hsing University, Taichung 402, TaiwanDepartment of Life Sciences, Agricultural Biotechnology Center, National Chung Hsing University, Taichung 402, TaiwanBotulinum toxin A (BoNT-A) is a bacterial zinc-dependent endopeptidase that acts specifically on neuromuscular junctions. BoNT-A blocks the release of acetylcholine, thereby decreasing the ability of a spastic muscle to generate forceful contraction, which results in a temporal local weakness and the atrophy of targeted muscles. BoNT-A-induced temporal muscle weakness has been used to manage skeletal muscle spasticity, such as poststroke spasticity, cerebral palsy, and cervical dystonia. However, the combined effect of treadmill exercise and BoNT-A treatment is not well understood. We previously demonstrated that for rats, following BoNT-A injection in the gastrocnemius muscle, treadmill running improved the recovery of the sciatic functional index (SFI), muscle contraction strength, and compound muscle action potential (CMAP) amplitude and area. Treadmill training had no influence on gastrocnemius mass that received BoNT-A injection, but it improved the maximal contraction force of the gastrocnemius, and upregulation of GAP-43, IGF-1, Myo-D, Myf-5, myogenin, and acetylcholine receptor (AChR) subunits α and β was found following treadmill training. Taken together, these results suggest that the upregulation of genes associated with neurite and AChR regeneration following treadmill training may contribute to enhanced gastrocnemius strength recovery following BoNT-A injection.http://dx.doi.org/10.1155/2013/593271
spellingShingle Sen-Wei Tsai
Hsiao-Ling Chen
Yi-Chun Chang
Chuan-Mu Chen
Molecular Mechanisms of Treadmill Therapy on Neuromuscular Atrophy Induced via Botulinum Toxin A
Neural Plasticity
title Molecular Mechanisms of Treadmill Therapy on Neuromuscular Atrophy Induced via Botulinum Toxin A
title_full Molecular Mechanisms of Treadmill Therapy on Neuromuscular Atrophy Induced via Botulinum Toxin A
title_fullStr Molecular Mechanisms of Treadmill Therapy on Neuromuscular Atrophy Induced via Botulinum Toxin A
title_full_unstemmed Molecular Mechanisms of Treadmill Therapy on Neuromuscular Atrophy Induced via Botulinum Toxin A
title_short Molecular Mechanisms of Treadmill Therapy on Neuromuscular Atrophy Induced via Botulinum Toxin A
title_sort molecular mechanisms of treadmill therapy on neuromuscular atrophy induced via botulinum toxin a
url http://dx.doi.org/10.1155/2013/593271
work_keys_str_mv AT senweitsai molecularmechanismsoftreadmilltherapyonneuromuscularatrophyinducedviabotulinumtoxina
AT hsiaolingchen molecularmechanismsoftreadmilltherapyonneuromuscularatrophyinducedviabotulinumtoxina
AT yichunchang molecularmechanismsoftreadmilltherapyonneuromuscularatrophyinducedviabotulinumtoxina
AT chuanmuchen molecularmechanismsoftreadmilltherapyonneuromuscularatrophyinducedviabotulinumtoxina