Effects of repeated intragastric administrations with heat-inactivated Mycobacterium aurum DSM 33539 on the stress-induced aggravation of dextran sulfate sodium (DSS) colitis in C57BL/6N mice
Stress-protective effects have been reported for M. vaccae NCTC 11659 and M. vaccae ATCC 15483T. However, it remains to be investigated whether also closely related rapidly growing environmental saprophytic non-tuberculous mycobacteria (NTM) species have protective effects against the negative conse...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnins.2024.1488603/full |
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| author | Dominik Langgartner Anna-Lena J. Weiss Mattia Amoroso John D. Sterrett Christopher A. Lowry Christopher A. Lowry Christopher A. Lowry Christopher A. Lowry Christopher A. Lowry Stefan O. Reber Stefan O. Reber |
| author_facet | Dominik Langgartner Anna-Lena J. Weiss Mattia Amoroso John D. Sterrett Christopher A. Lowry Christopher A. Lowry Christopher A. Lowry Christopher A. Lowry Christopher A. Lowry Stefan O. Reber Stefan O. Reber |
| author_sort | Dominik Langgartner |
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| description | Stress-protective effects have been reported for M. vaccae NCTC 11659 and M. vaccae ATCC 15483T. However, it remains to be investigated whether also closely related rapidly growing environmental saprophytic non-tuberculous mycobacteria (NTM) species have protective effects against the negative consequences of chronic psychosocial stress. Therefore, the aim of the current study was to assess whether repeated i.g. administrations of a heat-inactivated preparation of Mycobacterium aurum DSM 33539 prior to 19 days of chronic subordinate colony housing (CSC) are able to ameliorate the negative effects of this preclinically validated mouse model for chronic psychosocial stress on subsequent dextran sulfate sodium (DSS) colitis in male C57BL/6N mice. The results of the present study show that repeated i.g. administrations of M. aurum DSM 33539 have stabilizing effects on the composition of the gut microbiome, indicated by the findings that M. aurum DSM 33539 prevented CSC-induced increases in the relative abundances of the colitogenic phyla Desulfobacterota and Deferribacterota. Indeed, the relative abundance of Deferribacterota on day 19 was strongly correlated with histological damage to the colon. In line with the latter, M. aurum DSM 33539 was further protective against the aggravating effects of stress on subsequent DSS colitis. Collectively, our findings confirm and extend previous findings from our group and suggest that the stress-protective effects reported for M. vaccae NCTC 11659 and M. vaccae ATCC 15483T are generalizable also to other NTM species. |
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| institution | Kabale University |
| issn | 1662-453X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Neuroscience |
| spelling | doaj-art-cc2dce81ab734359a97cfe1d51fb938c2025-01-29T06:45:33ZengFrontiers Media S.A.Frontiers in Neuroscience1662-453X2025-01-011810.3389/fnins.2024.14886031488603Effects of repeated intragastric administrations with heat-inactivated Mycobacterium aurum DSM 33539 on the stress-induced aggravation of dextran sulfate sodium (DSS) colitis in C57BL/6N miceDominik Langgartner0Anna-Lena J. Weiss1Mattia Amoroso2John D. Sterrett3Christopher A. Lowry4Christopher A. Lowry5Christopher A. Lowry6Christopher A. Lowry7Christopher A. Lowry8Stefan O. Reber9Stefan O. Reber10Laboratory for Molecular Psychosomatics, Department of Psychosomatic Medicine and Psychotherapy, Ulm University Medical Center, Ulm, GermanyLaboratory for Molecular Psychosomatics, Department of Psychosomatic Medicine and Psychotherapy, Ulm University Medical Center, Ulm, GermanyLaboratory for Molecular Psychosomatics, Department of Psychosomatic Medicine and Psychotherapy, Ulm University Medical Center, Ulm, GermanyDepartment of Integrative Physiology, University of Colorado Boulder, Boulder, CO, United StatesDepartment of Integrative Physiology, University of Colorado Boulder, Boulder, CO, United StatesCenter for Neuroscience, University of Colorado Boulder, Boulder, CO, United StatesDepartment of Psychology and Neuroscience, University of Colorado Boulder, Boulder, CO, United StatesCenter for Microbial Exploration, University of Colorado Boulder, Boulder, CO, United StatesDepartment of Physical Medicine and Rehabilitation and Center for Neuroscience, University of Colorado Anschutz Medical Campus, Aurora, CO, United StatesLaboratory for Molecular Psychosomatics, Department of Psychosomatic Medicine and Psychotherapy, Ulm University Medical Center, Ulm, GermanyGerman Center for Mental Health (DZPG), Partner Site Mannheim//-Heidelberg//-Ulm, GermanyStress-protective effects have been reported for M. vaccae NCTC 11659 and M. vaccae ATCC 15483T. However, it remains to be investigated whether also closely related rapidly growing environmental saprophytic non-tuberculous mycobacteria (NTM) species have protective effects against the negative consequences of chronic psychosocial stress. Therefore, the aim of the current study was to assess whether repeated i.g. administrations of a heat-inactivated preparation of Mycobacterium aurum DSM 33539 prior to 19 days of chronic subordinate colony housing (CSC) are able to ameliorate the negative effects of this preclinically validated mouse model for chronic psychosocial stress on subsequent dextran sulfate sodium (DSS) colitis in male C57BL/6N mice. The results of the present study show that repeated i.g. administrations of M. aurum DSM 33539 have stabilizing effects on the composition of the gut microbiome, indicated by the findings that M. aurum DSM 33539 prevented CSC-induced increases in the relative abundances of the colitogenic phyla Desulfobacterota and Deferribacterota. Indeed, the relative abundance of Deferribacterota on day 19 was strongly correlated with histological damage to the colon. In line with the latter, M. aurum DSM 33539 was further protective against the aggravating effects of stress on subsequent DSS colitis. Collectively, our findings confirm and extend previous findings from our group and suggest that the stress-protective effects reported for M. vaccae NCTC 11659 and M. vaccae ATCC 15483T are generalizable also to other NTM species.https://www.frontiersin.org/articles/10.3389/fnins.2024.1488603/fullMycobacterium aurum DSM 33539intragastricchronic subordinate colony housing (CSC)Old Friendshygiene hypothesisresilience |
| spellingShingle | Dominik Langgartner Anna-Lena J. Weiss Mattia Amoroso John D. Sterrett Christopher A. Lowry Christopher A. Lowry Christopher A. Lowry Christopher A. Lowry Christopher A. Lowry Stefan O. Reber Stefan O. Reber Effects of repeated intragastric administrations with heat-inactivated Mycobacterium aurum DSM 33539 on the stress-induced aggravation of dextran sulfate sodium (DSS) colitis in C57BL/6N mice Frontiers in Neuroscience Mycobacterium aurum DSM 33539 intragastric chronic subordinate colony housing (CSC) Old Friends hygiene hypothesis resilience |
| title | Effects of repeated intragastric administrations with heat-inactivated Mycobacterium aurum DSM 33539 on the stress-induced aggravation of dextran sulfate sodium (DSS) colitis in C57BL/6N mice |
| title_full | Effects of repeated intragastric administrations with heat-inactivated Mycobacterium aurum DSM 33539 on the stress-induced aggravation of dextran sulfate sodium (DSS) colitis in C57BL/6N mice |
| title_fullStr | Effects of repeated intragastric administrations with heat-inactivated Mycobacterium aurum DSM 33539 on the stress-induced aggravation of dextran sulfate sodium (DSS) colitis in C57BL/6N mice |
| title_full_unstemmed | Effects of repeated intragastric administrations with heat-inactivated Mycobacterium aurum DSM 33539 on the stress-induced aggravation of dextran sulfate sodium (DSS) colitis in C57BL/6N mice |
| title_short | Effects of repeated intragastric administrations with heat-inactivated Mycobacterium aurum DSM 33539 on the stress-induced aggravation of dextran sulfate sodium (DSS) colitis in C57BL/6N mice |
| title_sort | effects of repeated intragastric administrations with heat inactivated mycobacterium aurum dsm 33539 on the stress induced aggravation of dextran sulfate sodium dss colitis in c57bl 6n mice |
| topic | Mycobacterium aurum DSM 33539 intragastric chronic subordinate colony housing (CSC) Old Friends hygiene hypothesis resilience |
| url | https://www.frontiersin.org/articles/10.3389/fnins.2024.1488603/full |
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