An inducible iFUCCI reporter cell line for tracking cell cycle dynamics in human pluripotent stem cells and their differentiated progeny
An ability to monitor cell cycle progression in real time has numerous applications in pluripotent stem cell-based models and therapeutics development. Fluorescence Ubiquitination-based Cell Cycle Indicator (FUCCI) systems enable live monitoring of the cell cycle in stem cells and their differentiat...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-12-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S187350612400312X |
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| author | Merve Ay Desi Veleva Mohammad Chowdhury Sonja Drljaca Thais Sobanski Andrew B.J. Prowse Dmitry A. Ovchinnikov |
| author_facet | Merve Ay Desi Veleva Mohammad Chowdhury Sonja Drljaca Thais Sobanski Andrew B.J. Prowse Dmitry A. Ovchinnikov |
| author_sort | Merve Ay |
| collection | DOAJ |
| description | An ability to monitor cell cycle progression in real time has numerous applications in pluripotent stem cell-based models and therapeutics development. Fluorescence Ubiquitination-based Cell Cycle Indicator (FUCCI) systems enable live monitoring of the cell cycle in stem cells and their differentiated progenies in a non-invasive manner. We describe the generation and characterisation of a cell line with a doxycycline-inducible reporter system, iFUCCI, in the human embryonic stem cell (hESC) line MEL-1. MEL-1 iFUCCI hESCs exhibited a normal karyotype, expressed markers of the undifferentiated state, and demonstrated expected differentiation potential by giving rise to cell populations from all three germ layers. |
| format | Article |
| id | doaj-art-cc2197864fb341eda940460d2f5809dd |
| institution | OA Journals |
| issn | 1873-5061 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj-art-cc2197864fb341eda940460d2f5809dd2025-08-20T02:38:14ZengElsevierStem Cell Research1873-50612024-12-018110361410.1016/j.scr.2024.103614An inducible iFUCCI reporter cell line for tracking cell cycle dynamics in human pluripotent stem cells and their differentiated progenyMerve Ay0Desi Veleva1Mohammad Chowdhury2Sonja Drljaca3Thais Sobanski4Andrew B.J. Prowse5Dmitry A. Ovchinnikov6StemCore, The Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia 4072, QLD, AustraliaStemCore, The Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia 4072, QLD, Australia; Corresponding authors at: StemCore, The Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia 4072, QLD, Australia (D.A. Ovchinnikov).StemCore, The Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia 4072, QLD, AustraliaStemCore, The Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia 4072, QLD, AustraliaStemCore, The Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia 4072, QLD, AustraliaStemCore, The Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia 4072, QLD, AustraliaStemCore, The Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia 4072, QLD, Australia; The Florey, The University of Melbourne, 30 Royal Parade, Melbourne, VIC 3010, Australia; Corresponding authors at: StemCore, The Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia 4072, QLD, Australia (D.A. Ovchinnikov).An ability to monitor cell cycle progression in real time has numerous applications in pluripotent stem cell-based models and therapeutics development. Fluorescence Ubiquitination-based Cell Cycle Indicator (FUCCI) systems enable live monitoring of the cell cycle in stem cells and their differentiated progenies in a non-invasive manner. We describe the generation and characterisation of a cell line with a doxycycline-inducible reporter system, iFUCCI, in the human embryonic stem cell (hESC) line MEL-1. MEL-1 iFUCCI hESCs exhibited a normal karyotype, expressed markers of the undifferentiated state, and demonstrated expected differentiation potential by giving rise to cell populations from all three germ layers.http://www.sciencedirect.com/science/article/pii/S187350612400312X |
| spellingShingle | Merve Ay Desi Veleva Mohammad Chowdhury Sonja Drljaca Thais Sobanski Andrew B.J. Prowse Dmitry A. Ovchinnikov An inducible iFUCCI reporter cell line for tracking cell cycle dynamics in human pluripotent stem cells and their differentiated progeny Stem Cell Research |
| title | An inducible iFUCCI reporter cell line for tracking cell cycle dynamics in human pluripotent stem cells and their differentiated progeny |
| title_full | An inducible iFUCCI reporter cell line for tracking cell cycle dynamics in human pluripotent stem cells and their differentiated progeny |
| title_fullStr | An inducible iFUCCI reporter cell line for tracking cell cycle dynamics in human pluripotent stem cells and their differentiated progeny |
| title_full_unstemmed | An inducible iFUCCI reporter cell line for tracking cell cycle dynamics in human pluripotent stem cells and their differentiated progeny |
| title_short | An inducible iFUCCI reporter cell line for tracking cell cycle dynamics in human pluripotent stem cells and their differentiated progeny |
| title_sort | inducible ifucci reporter cell line for tracking cell cycle dynamics in human pluripotent stem cells and their differentiated progeny |
| url | http://www.sciencedirect.com/science/article/pii/S187350612400312X |
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