An Evaluation of the Levels of IL-1β and IL-7 in Children With Viral Gastroenteritis

Gastroenteritis is associated with high rates of morbidity and mortality in the general population, especially in children. To investigate the predictive role of interleukins (IL-1β and IL-7) in children with viral gastroenteritis as potential diagnostic biomarkers. This cross-sectional study inclu...

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Main Authors: Saja Mohammed Mohsen, Asmaa Haseeb Hwaid, Maha Falih Nazzal
Format: Article
Language:English
Published: Tehran University of Medical Sciences 2025-05-01
Series:Acta Medica Iranica
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Online Access:https://acta.tums.ac.ir/index.php/acta/article/view/11365
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author Saja Mohammed Mohsen
Asmaa Haseeb Hwaid
Maha Falih Nazzal
author_facet Saja Mohammed Mohsen
Asmaa Haseeb Hwaid
Maha Falih Nazzal
author_sort Saja Mohammed Mohsen
collection DOAJ
description Gastroenteritis is associated with high rates of morbidity and mortality in the general population, especially in children. To investigate the predictive role of interleukins (IL-1β and IL-7) in children with viral gastroenteritis as potential diagnostic biomarkers. This cross-sectional study included children who were diagnosed with gastroenteritis at private clinics and Al-Batoul Teaching Hospital for Maternity and Childhood, Diyala, Iraq. The present study was conducted from May 2023 to May 2024. Ninety blood samples were collected from children with gastroenteritis, and 50 blood samples were taken from healthy children and those in the control group. Additionally, 90 samples of the stool were collected. The ELIZA technique was used for exploring IL-1β and IL-7 in blood samples and for detecting viral antigens (Norovirus, Adenovirus and Astrovirus) in stool samples. The data were analyzed statistically via SPSS v. 24 and Prism v. 10. There was a statistically significant increase in the incidence of infectious gastroenteritis in thin children aged 1-3 years who were living in rural areas. As indicated in this study, the most common symptoms were diarrhoea, vomiting and fever, and the differences were significant. Compared with those in the control group, there were significant differences in the levels of IL-1β and IL-7 among the children infected with gastroenteritis. ROC analysis of IL-1β and IL-7 interleukins revealed moderate sensitivity (79% and 88%, respectively) and low specificity (64% and 69%, respectively) at cut-off values of 8.52 and 7.01. The occurrence of norovirus occurred in 30% of the total samples, followed by Adenovirus in 20% and Astrovirus in 11%. The serum levels of IL-1β and IL-7 were high among children infected with gastroenteritis. Nevertheless, these parameters cannot be considered reliable vital indicators that are sufficient for diagnosing infections caused by gastroenteritis.
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spelling doaj-art-cc1e395028ca433ebd5e59a469fe51c52025-08-20T03:10:18ZengTehran University of Medical SciencesActa Medica Iranica0044-60251735-96942025-05-0163110.18502/acta.v63i1.18590An Evaluation of the Levels of IL-1β and IL-7 in Children With Viral GastroenteritisSaja Mohammed Mohsen0Asmaa Haseeb Hwaid1Maha Falih Nazzal2Department of Medical Laboratories Techniques, Baqubah-Technical College, Middle Technical University, Diyala, Iraq.Department of Biology, College of Education for Pure Sciences, University of Diyala, Diyala, Iraq.Department of Biology, College of Education for Pure Sciences, University of Diyala, Diyala, Iraq. Gastroenteritis is associated with high rates of morbidity and mortality in the general population, especially in children. To investigate the predictive role of interleukins (IL-1β and IL-7) in children with viral gastroenteritis as potential diagnostic biomarkers. This cross-sectional study included children who were diagnosed with gastroenteritis at private clinics and Al-Batoul Teaching Hospital for Maternity and Childhood, Diyala, Iraq. The present study was conducted from May 2023 to May 2024. Ninety blood samples were collected from children with gastroenteritis, and 50 blood samples were taken from healthy children and those in the control group. Additionally, 90 samples of the stool were collected. The ELIZA technique was used for exploring IL-1β and IL-7 in blood samples and for detecting viral antigens (Norovirus, Adenovirus and Astrovirus) in stool samples. The data were analyzed statistically via SPSS v. 24 and Prism v. 10. There was a statistically significant increase in the incidence of infectious gastroenteritis in thin children aged 1-3 years who were living in rural areas. As indicated in this study, the most common symptoms were diarrhoea, vomiting and fever, and the differences were significant. Compared with those in the control group, there were significant differences in the levels of IL-1β and IL-7 among the children infected with gastroenteritis. ROC analysis of IL-1β and IL-7 interleukins revealed moderate sensitivity (79% and 88%, respectively) and low specificity (64% and 69%, respectively) at cut-off values of 8.52 and 7.01. The occurrence of norovirus occurred in 30% of the total samples, followed by Adenovirus in 20% and Astrovirus in 11%. The serum levels of IL-1β and IL-7 were high among children infected with gastroenteritis. Nevertheless, these parameters cannot be considered reliable vital indicators that are sufficient for diagnosing infections caused by gastroenteritis. https://acta.tums.ac.ir/index.php/acta/article/view/11365GastroenteritisViral infectionsInterleukinsInterleukin-1 beta (IL-1 β)Interleukin-7 (IL-7)
spellingShingle Saja Mohammed Mohsen
Asmaa Haseeb Hwaid
Maha Falih Nazzal
An Evaluation of the Levels of IL-1β and IL-7 in Children With Viral Gastroenteritis
Acta Medica Iranica
Gastroenteritis
Viral infections
Interleukins
Interleukin-1 beta (IL-1 β)
Interleukin-7 (IL-7)
title An Evaluation of the Levels of IL-1β and IL-7 in Children With Viral Gastroenteritis
title_full An Evaluation of the Levels of IL-1β and IL-7 in Children With Viral Gastroenteritis
title_fullStr An Evaluation of the Levels of IL-1β and IL-7 in Children With Viral Gastroenteritis
title_full_unstemmed An Evaluation of the Levels of IL-1β and IL-7 in Children With Viral Gastroenteritis
title_short An Evaluation of the Levels of IL-1β and IL-7 in Children With Viral Gastroenteritis
title_sort evaluation of the levels of il 1β and il 7 in children with viral gastroenteritis
topic Gastroenteritis
Viral infections
Interleukins
Interleukin-1 beta (IL-1 β)
Interleukin-7 (IL-7)
url https://acta.tums.ac.ir/index.php/acta/article/view/11365
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