Hemocyanin controls Vibrio levels in hemolymph by modulating intracellular ROS via p38 MAPK
Redox reactions are essential for various biological processes, hence, maintaining redox homeostasis is critical for cellular health and host–microbe interactions, particularly in marine invertebrates, where these processes remain poorly understood. This study investigates the role of the respirator...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Virulence |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21505594.2025.2546066 |
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| author | Jiawei Cheng Yunyu Shi Yonghui Kong Yuxi Wu Yankang Wang Jude Juventus Aweya Shiyuan Bao Junjie Nie Qian Feng Yongzhen Zhao Yueling Zhang Zhihong Zheng |
| author_facet | Jiawei Cheng Yunyu Shi Yonghui Kong Yuxi Wu Yankang Wang Jude Juventus Aweya Shiyuan Bao Junjie Nie Qian Feng Yongzhen Zhao Yueling Zhang Zhihong Zheng |
| author_sort | Jiawei Cheng |
| collection | DOAJ |
| description | Redox reactions are essential for various biological processes, hence, maintaining redox homeostasis is critical for cellular health and host–microbe interactions, particularly in marine invertebrates, where these processes remain poorly understood. This study investigates the role of the respiratory protein hemocyanin in regulating redox-related genes, reactive oxygen species (ROS) levels in shrimp hemocytes, and its influence on immune function and hemolymph Vibrio abundance. Our results show that hemocyanin is crucial in the regulation of the redox system, as hemocyanin knockdown disrupts the expression of antioxidant (PvPOD and PvGPX) and pro-oxidant (PvXOX) genes and redox enzyme activities, leading to elevated ROS levels in hemocytes. Additionally, we found that hemocyanin controls redox homeostasis in hemocytes through the p38-MAPK-c-Jun signaling pathway. Moreover, ROS levels regulated by hemocyanin via the MAPK pathway impacted on hemolymph Vibrio spp. abundance. These findings highlight a crucial role of hemocyanin as an antioxidant that modulates redox balance in shrimp hemocytes, with significant implications for immune defense and microbial homeostasis, offering new insights into antioxidative mechanisms in marine invertebrates. |
| format | Article |
| id | doaj-art-cc1bf64cb6ee489fb167420a150c1c7f |
| institution | Kabale University |
| issn | 2150-5594 2150-5608 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Virulence |
| spelling | doaj-art-cc1bf64cb6ee489fb167420a150c1c7f2025-08-25T11:36:07ZengTaylor & Francis GroupVirulence2150-55942150-56082025-12-0116110.1080/21505594.2025.2546066Hemocyanin controls Vibrio levels in hemolymph by modulating intracellular ROS via p38 MAPKJiawei Cheng0Yunyu Shi1Yonghui Kong2Yuxi Wu3Yankang Wang4Jude Juventus Aweya5Shiyuan Bao6Junjie Nie7Qian Feng8Yongzhen Zhao9Yueling Zhang10Zhihong Zheng11Guangdong Provincial Key Laboratory of Marine Biotechnology, Institute of Marine Sciences, Shantou University, Shantou, ChinaGuangdong Provincial Key Laboratory of Marine Biotechnology, Institute of Marine Sciences, Shantou University, Shantou, ChinaGuangdong Provincial Key Laboratory of Marine Biotechnology, Institute of Marine Sciences, Shantou University, Shantou, ChinaGuangdong Provincial Key Laboratory of Marine Biotechnology, Institute of Marine Sciences, Shantou University, Shantou, ChinaGuangdong Provincial Key Laboratory of Marine Biotechnology, Institute of Marine Sciences, Shantou University, Shantou, ChinaGuangdong Provincial Key Laboratory of Marine Biotechnology, Institute of Marine Sciences, Shantou University, Shantou, ChinaGuangdong Provincial Key Laboratory of Marine Biotechnology, Institute of Marine Sciences, Shantou University, Shantou, ChinaGuangdong Provincial Key Laboratory of Marine Biotechnology, Institute of Marine Sciences, Shantou University, Shantou, ChinaGuangdong Provincial Key Laboratory of Marine Biotechnology, Institute of Marine Sciences, Shantou University, Shantou, ChinaGuangxi Academy of Fishery Sciences, Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Nanning, ChinaGuangdong Provincial Key Laboratory of Marine Biotechnology, Institute of Marine Sciences, Shantou University, Shantou, ChinaGuangdong Provincial Key Laboratory of Marine Biotechnology, Institute of Marine Sciences, Shantou University, Shantou, ChinaRedox reactions are essential for various biological processes, hence, maintaining redox homeostasis is critical for cellular health and host–microbe interactions, particularly in marine invertebrates, where these processes remain poorly understood. This study investigates the role of the respiratory protein hemocyanin in regulating redox-related genes, reactive oxygen species (ROS) levels in shrimp hemocytes, and its influence on immune function and hemolymph Vibrio abundance. Our results show that hemocyanin is crucial in the regulation of the redox system, as hemocyanin knockdown disrupts the expression of antioxidant (PvPOD and PvGPX) and pro-oxidant (PvXOX) genes and redox enzyme activities, leading to elevated ROS levels in hemocytes. Additionally, we found that hemocyanin controls redox homeostasis in hemocytes through the p38-MAPK-c-Jun signaling pathway. Moreover, ROS levels regulated by hemocyanin via the MAPK pathway impacted on hemolymph Vibrio spp. abundance. These findings highlight a crucial role of hemocyanin as an antioxidant that modulates redox balance in shrimp hemocytes, with significant implications for immune defense and microbial homeostasis, offering new insights into antioxidative mechanisms in marine invertebrates.https://www.tandfonline.com/doi/10.1080/21505594.2025.2546066Shrimp hemocyaninredox balanceROSp38-MAPK-c-jun pathwayhemolymph Vibrio abundance |
| spellingShingle | Jiawei Cheng Yunyu Shi Yonghui Kong Yuxi Wu Yankang Wang Jude Juventus Aweya Shiyuan Bao Junjie Nie Qian Feng Yongzhen Zhao Yueling Zhang Zhihong Zheng Hemocyanin controls Vibrio levels in hemolymph by modulating intracellular ROS via p38 MAPK Virulence Shrimp hemocyanin redox balance ROS p38-MAPK-c-jun pathway hemolymph Vibrio abundance |
| title | Hemocyanin controls Vibrio levels in hemolymph by modulating intracellular ROS via p38 MAPK |
| title_full | Hemocyanin controls Vibrio levels in hemolymph by modulating intracellular ROS via p38 MAPK |
| title_fullStr | Hemocyanin controls Vibrio levels in hemolymph by modulating intracellular ROS via p38 MAPK |
| title_full_unstemmed | Hemocyanin controls Vibrio levels in hemolymph by modulating intracellular ROS via p38 MAPK |
| title_short | Hemocyanin controls Vibrio levels in hemolymph by modulating intracellular ROS via p38 MAPK |
| title_sort | hemocyanin controls vibrio levels in hemolymph by modulating intracellular ros via p38 mapk |
| topic | Shrimp hemocyanin redox balance ROS p38-MAPK-c-jun pathway hemolymph Vibrio abundance |
| url | https://www.tandfonline.com/doi/10.1080/21505594.2025.2546066 |
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