Virulence Genes and Antimicrobial Resistance Profiles of Pasteurella multocida Strains Isolated from Rabbits in Brazil
Pasteurella multocida is responsible for a wide range of diseases in domestic animals. In rabbits, the agent is related to nasal discharge, pneumonia, otitis media, pyometra, orchitis, abscess, and septicemia. One hundred and forty rabbits with respiratory diseases from four rabbitries in São Paulo...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2012-01-01
|
| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/685028 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832562976190627840 |
|---|---|
| author | Thais Sebastiana Porfida Ferreira Maria Roberta Felizardo Débora Dirani Sena de Gobbi Cleise Ribeiro Gomes Pedro Henrique de Lima Nogueira Filsner Marina Moreno Renata Paixão Jucélia de Jesus Pereira Andrea Micke Moreno |
| author_facet | Thais Sebastiana Porfida Ferreira Maria Roberta Felizardo Débora Dirani Sena de Gobbi Cleise Ribeiro Gomes Pedro Henrique de Lima Nogueira Filsner Marina Moreno Renata Paixão Jucélia de Jesus Pereira Andrea Micke Moreno |
| author_sort | Thais Sebastiana Porfida Ferreira |
| collection | DOAJ |
| description | Pasteurella multocida is responsible for a wide range of diseases in domestic animals. In rabbits, the agent is related to nasal discharge, pneumonia, otitis media, pyometra, orchitis, abscess, and septicemia. One hundred and forty rabbits with respiratory diseases from four rabbitries in São Paulo State, Brazil were evaluated for the detection of P. multocida in their nasal cavities. A total of twenty-nine animals were positive to P. multocida isolation, and 46 strains were selected and characterized by means of biochemical tests and PCR. P. multocida strains were tested for capsular type, virulence genes, and resistance profile. A total of 45.6% (21/46) of isolates belonged to capsular type A, and 54.34% (25/46) of the isolates were untypeable. None of the strains harboured toxA or pfhA genes. The frequency of the other twenty genes tested was variable, and the data generated was used to build a dendrogram, showing the relatedness of strains, which were clustered according to origin. Resistance revealed to be more common against sulfonamides and cotrimoxazole, followed by erythromycin, penicillin, and amoxicillin. |
| format | Article |
| id | doaj-art-cc1972db045645d59563aef7b1a800db |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-cc1972db045645d59563aef7b1a800db2025-02-03T01:21:21ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/685028685028Virulence Genes and Antimicrobial Resistance Profiles of Pasteurella multocida Strains Isolated from Rabbits in BrazilThais Sebastiana Porfida Ferreira0Maria Roberta Felizardo1Débora Dirani Sena de Gobbi2Cleise Ribeiro Gomes3Pedro Henrique de Lima Nogueira Filsner4Marina Moreno5Renata Paixão6Jucélia de Jesus Pereira7Andrea Micke Moreno8Programa de Epidemiologia Experimental Aplicada às Zoonoses, Laboratório de Sanidade Suína e Virologia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, BrazilPrograma de Epidemiologia Experimental Aplicada às Zoonoses, Laboratório de Sanidade Suína e Virologia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, BrazilPrograma de Epidemiologia Experimental Aplicada às Zoonoses, Laboratório de Sanidade Suína e Virologia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, BrazilPrograma de Epidemiologia Experimental Aplicada às Zoonoses, Laboratório de Sanidade Suína e Virologia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, BrazilPrograma de Epidemiologia Experimental Aplicada às Zoonoses, Laboratório de Sanidade Suína e Virologia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, BrazilPrograma de Epidemiologia Experimental Aplicada às Zoonoses, Laboratório de Sanidade Suína e Virologia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, BrazilPrograma de Epidemiologia Experimental Aplicada às Zoonoses, Laboratório de Sanidade Suína e Virologia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, BrazilPrograma de Epidemiologia Experimental Aplicada às Zoonoses, Laboratório de Sanidade Suína e Virologia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, BrazilPrograma de Epidemiologia Experimental Aplicada às Zoonoses, Laboratório de Sanidade Suína e Virologia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, BrazilPasteurella multocida is responsible for a wide range of diseases in domestic animals. In rabbits, the agent is related to nasal discharge, pneumonia, otitis media, pyometra, orchitis, abscess, and septicemia. One hundred and forty rabbits with respiratory diseases from four rabbitries in São Paulo State, Brazil were evaluated for the detection of P. multocida in their nasal cavities. A total of twenty-nine animals were positive to P. multocida isolation, and 46 strains were selected and characterized by means of biochemical tests and PCR. P. multocida strains were tested for capsular type, virulence genes, and resistance profile. A total of 45.6% (21/46) of isolates belonged to capsular type A, and 54.34% (25/46) of the isolates were untypeable. None of the strains harboured toxA or pfhA genes. The frequency of the other twenty genes tested was variable, and the data generated was used to build a dendrogram, showing the relatedness of strains, which were clustered according to origin. Resistance revealed to be more common against sulfonamides and cotrimoxazole, followed by erythromycin, penicillin, and amoxicillin.http://dx.doi.org/10.1100/2012/685028 |
| spellingShingle | Thais Sebastiana Porfida Ferreira Maria Roberta Felizardo Débora Dirani Sena de Gobbi Cleise Ribeiro Gomes Pedro Henrique de Lima Nogueira Filsner Marina Moreno Renata Paixão Jucélia de Jesus Pereira Andrea Micke Moreno Virulence Genes and Antimicrobial Resistance Profiles of Pasteurella multocida Strains Isolated from Rabbits in Brazil The Scientific World Journal |
| title | Virulence Genes and Antimicrobial Resistance Profiles of Pasteurella multocida Strains Isolated from Rabbits in Brazil |
| title_full | Virulence Genes and Antimicrobial Resistance Profiles of Pasteurella multocida Strains Isolated from Rabbits in Brazil |
| title_fullStr | Virulence Genes and Antimicrobial Resistance Profiles of Pasteurella multocida Strains Isolated from Rabbits in Brazil |
| title_full_unstemmed | Virulence Genes and Antimicrobial Resistance Profiles of Pasteurella multocida Strains Isolated from Rabbits in Brazil |
| title_short | Virulence Genes and Antimicrobial Resistance Profiles of Pasteurella multocida Strains Isolated from Rabbits in Brazil |
| title_sort | virulence genes and antimicrobial resistance profiles of pasteurella multocida strains isolated from rabbits in brazil |
| url | http://dx.doi.org/10.1100/2012/685028 |
| work_keys_str_mv | AT thaissebastianaporfidaferreira virulencegenesandantimicrobialresistanceprofilesofpasteurellamultocidastrainsisolatedfromrabbitsinbrazil AT mariarobertafelizardo virulencegenesandantimicrobialresistanceprofilesofpasteurellamultocidastrainsisolatedfromrabbitsinbrazil AT deboradiranisenadegobbi virulencegenesandantimicrobialresistanceprofilesofpasteurellamultocidastrainsisolatedfromrabbitsinbrazil AT cleiseribeirogomes virulencegenesandantimicrobialresistanceprofilesofpasteurellamultocidastrainsisolatedfromrabbitsinbrazil AT pedrohenriquedelimanogueirafilsner virulencegenesandantimicrobialresistanceprofilesofpasteurellamultocidastrainsisolatedfromrabbitsinbrazil AT marinamoreno virulencegenesandantimicrobialresistanceprofilesofpasteurellamultocidastrainsisolatedfromrabbitsinbrazil AT renatapaixao virulencegenesandantimicrobialresistanceprofilesofpasteurellamultocidastrainsisolatedfromrabbitsinbrazil AT juceliadejesuspereira virulencegenesandantimicrobialresistanceprofilesofpasteurellamultocidastrainsisolatedfromrabbitsinbrazil AT andreamickemoreno virulencegenesandantimicrobialresistanceprofilesofpasteurellamultocidastrainsisolatedfromrabbitsinbrazil |