Immunoenhancement on DNA vaccine of polyprotein gene in infectious bursal disease virus by co-delivery with plasmid encoding chicken interleukin-18
To further evaluate the immunoenhancement of chicken interleukin-18 (IL-18), the polyprotein of infectious bursal disease virus (IBDV) was prepared to DNA vaccine, and the DNA vaccine was coadministrated with the eukaryotic expression plasmid encoding interleukin-18 (pCI-IL-18), then boosted after 2...
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| Format: | Article |
| Language: | English |
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Zhejiang University Press
2008-01-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2008.01.003 |
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| _version_ | 1849413460378517504 |
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| author | YU Xia-meng SHOU Chun-bo ZHANG Xiao-dong XU Gen-liang YU Lian |
| author_facet | YU Xia-meng SHOU Chun-bo ZHANG Xiao-dong XU Gen-liang YU Lian |
| author_sort | YU Xia-meng |
| collection | DOAJ |
| description | To further evaluate the immunoenhancement of chicken interleukin-18 (IL-18), the polyprotein of infectious bursal disease virus (IBDV) was prepared to DNA vaccine, and the DNA vaccine was coadministrated with the eukaryotic expression plasmid encoding interleukin-18 (pCI-IL-18), then boosted after 2 weeks, and challenged with virulent IBDV 5 weeks later. It is observed that chicken IL-18 could significantly enhance the proliferation responses of T lymphocytes from spleen and B lymphocytes from bursa. Meanwhile, it can raise the neutralization antibody level and enhance the protection against virulent IBDV induced by DNA vaccine. The results indicate the strong enhancement of immune responses to IBDV DNA vaccine by co-administration with chicken IL-18 (200 µg) (P<0.05). |
| format | Article |
| id | doaj-art-cc17e7d086c440e6aebc09e3c0628dc5 |
| institution | Kabale University |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2008-01-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-cc17e7d086c440e6aebc09e3c0628dc52025-08-20T03:34:06ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552008-01-0134131810.3785/j.issn.1008-9209.2008.01.00310089209Immunoenhancement on DNA vaccine of polyprotein gene in infectious bursal disease virus by co-delivery with plasmid encoding chicken interleukin-18YU Xia-mengSHOU Chun-boZHANG Xiao-dongXU Gen-liangYU LianTo further evaluate the immunoenhancement of chicken interleukin-18 (IL-18), the polyprotein of infectious bursal disease virus (IBDV) was prepared to DNA vaccine, and the DNA vaccine was coadministrated with the eukaryotic expression plasmid encoding interleukin-18 (pCI-IL-18), then boosted after 2 weeks, and challenged with virulent IBDV 5 weeks later. It is observed that chicken IL-18 could significantly enhance the proliferation responses of T lymphocytes from spleen and B lymphocytes from bursa. Meanwhile, it can raise the neutralization antibody level and enhance the protection against virulent IBDV induced by DNA vaccine. The results indicate the strong enhancement of immune responses to IBDV DNA vaccine by co-administration with chicken IL-18 (200 µg) (P<0.05).https://www.academax.com/doi/10.3785/j.issn.1008-9209.2008.01.003chicken interleukin-18 (IL-18)infectious bursal disease virus (IBDV)DNA vaccineimmunoenhancement |
| spellingShingle | YU Xia-meng SHOU Chun-bo ZHANG Xiao-dong XU Gen-liang YU Lian Immunoenhancement on DNA vaccine of polyprotein gene in infectious bursal disease virus by co-delivery with plasmid encoding chicken interleukin-18 浙江大学学报. 农业与生命科学版 chicken interleukin-18 (IL-18) infectious bursal disease virus (IBDV) DNA vaccine immunoenhancement |
| title | Immunoenhancement on DNA vaccine of polyprotein gene in infectious bursal disease virus by co-delivery with plasmid encoding chicken interleukin-18 |
| title_full | Immunoenhancement on DNA vaccine of polyprotein gene in infectious bursal disease virus by co-delivery with plasmid encoding chicken interleukin-18 |
| title_fullStr | Immunoenhancement on DNA vaccine of polyprotein gene in infectious bursal disease virus by co-delivery with plasmid encoding chicken interleukin-18 |
| title_full_unstemmed | Immunoenhancement on DNA vaccine of polyprotein gene in infectious bursal disease virus by co-delivery with plasmid encoding chicken interleukin-18 |
| title_short | Immunoenhancement on DNA vaccine of polyprotein gene in infectious bursal disease virus by co-delivery with plasmid encoding chicken interleukin-18 |
| title_sort | immunoenhancement on dna vaccine of polyprotein gene in infectious bursal disease virus by co delivery with plasmid encoding chicken interleukin 18 |
| topic | chicken interleukin-18 (IL-18) infectious bursal disease virus (IBDV) DNA vaccine immunoenhancement |
| url | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2008.01.003 |
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